Regeneration and experimental orthotopic transplantation of a bioengineered kidney
Jeremy Song,Jacques P. Guyette,Sarah E. Gilpin,Gabriel Gonzalez,Joseph P. Vacanti,Harald C. Ott +5 more
Reads0
Chats0
TLDR
To regenerate functional tissue, rat kidney scaffolds are seeded with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor, resulting in grafts that produced rudimentary urine in vitro when perfused through their intrinsic vascular bed.Abstract:
Approximately 100,000 individuals in the United States currently await kidney transplantation, and 400,000 individuals live with end-stage kidney disease requiring hemodialysis. The creation of a transplantable graft to permanently replace kidney function would address donor organ shortage and the morbidity associated with immunosuppression. Such a bioengineered graft must have the kidney's architecture and function and permit perfusion, filtration, secretion, absorption and drainage of urine. We decellularized rat, porcine and human kidneys by detergent perfusion, yielding acellular scaffolds with vascular, cortical and medullary architecture, a collecting system and ureters. To regenerate functional tissue, we seeded rat kidney scaffolds with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor. The resulting grafts produced rudimentary urine in vitro when perfused through their intrinsic vascular bed. When transplanted in an orthotopic position in rat, the grafts were perfused by the recipient's circulation and produced urine through the ureteral conduit in vivo.read more
Citations
More filters
Journal ArticleDOI
The bridge between transplantation and regenerative medicine: Beginning a new Banff classification of tissue engineering pathology.
Kim Solez,K. C. Fung,K. A. Saliba,V. L. C. Sheldon,Astgik Petrosyan,Laura Perin,James F. Burdick,W. H. Fissell,Anthony J. Demetris,Lynn D. Cornell +9 more
TL;DR: A new Banff classification of tissue engineering pathology is proposed to standardize and assess de novo bioengineered solid organs transplantable success in vivo and a possible nosology of pathologic abnormalities in tissue‐engineered organs is suggested.
Journal ArticleDOI
Kidney development and perspectives for organ engineering.
TL;DR: This work discusses signaling in renal development and the composition of the renal extracellular matrix before presenting progress in the decellularization and 3D bioprinting fields.
Journal ArticleDOI
Biomimetic fetal rotation bioreactor for engineering bone tissues-Effect of cyclic strains on upregulation of osteogenic gene expression.
Akhilandeshwari Ravichandran,Feng Wen,Jing Lim,Mark Seow Khoon Chong,Jerry Kok Yen Chan,Jerry Kok Yen Chan,Swee Hin Teoh +6 more
TL;DR: The independent and combined effects of mechanical stimulation and biaxial rotation for bone tissue engineering using a bioreactor platform with non‐invasive sensing modalities are reported for the first time.
Journal ArticleDOI
Evaluation and preservation of vascular architectures in decellularized whole rat kidneys.
TL;DR: A Micro-CT imaging method for evaluating the integrity of vasculature of the decellularized whole organ scaffolds with/without freezing/thawing is reported, which confirms various structural alterations in the vasculatures of the whole de cellularized kidney matrix with/ without frozen storage.
Patent
Organoids comprising isolated renal cells and uses thereof
TL;DR: In this paper, the authors describe organoids comprising of selected bioactive primary renal cells and a bioactive cell population, e.g., an endothelial cell populations, and methods of treating a subject in need thereof with such organoids.
References
More filters
Journal ArticleDOI
Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Harald C. Ott,Thomas S Matthiesen,Saik Kia Goh,Lauren D. Black,Stefan M. Kren,Theoden I. Netoff,Doris A. Taylor +6 more
TL;DR: Eight constructs decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular architecture, competent a cellular valves and intact chamber geometry that could generate pump function in a modified working heart preparation.
Journal ArticleDOI
Tissue-engineered autologous bladders for patients needing cystoplasty
TL;DR: Engineered bladder tissues, created with autologous cells seeded on collagen-polyglycolic acid scaffolds, and wrapped in omentum after implantation, can be used in patients who need cystoplasty.
Journal ArticleDOI
Regeneration and orthotopic transplantation of a bioartificial lung
Harald C. Ott,Ben Clippinger,Claudius Conrad,Christian Schuetz,Irina Pomerantseva,Laertis Ikonomou,Darrell N. Kotton,Joseph P. Vacanti +7 more
TL;DR: Creation of a bioartificial lung requires engineering of viable lung architecture enabling ventilation, perfusion and gas exchange, and regenerated lungs into orthotopic position showed in vivo function.
Journal ArticleDOI
HLA-Mismatched Renal Transplantation without Maintenance Immunosuppression
Tatsuo Kawai,A. Benedict Cosimi,Thomas R. Spitzer,Nina Tolkoff-Rubin,Manikkam Suthanthiran,Susan L. Saidman,Juanita Shaffer,Frederic I. Preffer,Ruchuang Ding,Vijay K. Sharma,Jay A. Fishman,Bimalangshu R. Dey,Dicken S.C. Ko,Martin Hertl,Nelson Goes,Waichi Wong,Winfred W. Williams,Robert B. Colvin,Megan Sykes,David H. Sachs +19 more
TL;DR: Five patients with end-stage renal disease received combined bone marrow and kidney transplants from HLA single-haplotype mismatched living related donors, with the use of a nonmyeloablative preparative regimen, and it was possible to discontinue all immunosuppressive therapy 9 to 14 months after the transplantation.
Journal ArticleDOI
Organ engineering based on decellularized matrix scaffolds
Jeremy Song,Harald C. Ott +1 more
TL;DR: A review summarizes achievements to date and discusses the role of native ECM scaffolds in organ regeneration, which provides a promising alternative to synthetic scaffolds and a foundation for regenerative efforts.
Related Papers (5)
Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix
Basak E. Uygun,Alejandro Soto-Gutierrez,Alejandro Soto-Gutierrez,Hiroshi Yagi,Hiroshi Yagi,Maria Louisa Izamis,Maria Angela Guzzardi,Maria Angela Guzzardi,Carley Shulman,Jack M. Milwid,Naoya Kobayashi,Arno W. Tilles,Francois Berthiaume,Francois Berthiaume,Martin Hertl,Yaakov Nahmias,Yaakov Nahmias,Martin L. Yarmush,Martin L. Yarmush,Korkut Uygun +19 more