Regeneration and experimental orthotopic transplantation of a bioengineered kidney
Jeremy Song,Jacques P. Guyette,Sarah E. Gilpin,Gabriel Gonzalez,Joseph P. Vacanti,Harald C. Ott +5 more
Reads0
Chats0
TLDR
To regenerate functional tissue, rat kidney scaffolds are seeded with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor, resulting in grafts that produced rudimentary urine in vitro when perfused through their intrinsic vascular bed.Abstract:
Approximately 100,000 individuals in the United States currently await kidney transplantation, and 400,000 individuals live with end-stage kidney disease requiring hemodialysis. The creation of a transplantable graft to permanently replace kidney function would address donor organ shortage and the morbidity associated with immunosuppression. Such a bioengineered graft must have the kidney's architecture and function and permit perfusion, filtration, secretion, absorption and drainage of urine. We decellularized rat, porcine and human kidneys by detergent perfusion, yielding acellular scaffolds with vascular, cortical and medullary architecture, a collecting system and ureters. To regenerate functional tissue, we seeded rat kidney scaffolds with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor. The resulting grafts produced rudimentary urine in vitro when perfused through their intrinsic vascular bed. When transplanted in an orthotopic position in rat, the grafts were perfused by the recipient's circulation and produced urine through the ureteral conduit in vivo.read more
Citations
More filters
Journal ArticleDOI
Creation and implantation of acellular rat renal ECM-based scaffolds
Andrea Peloso,Jacopo Ferrario,Benedetta Maiga,Ilaria Benzoni,Carolina Bianco,Antonio Citro,Manuela Currao,Alessandro Malara,Gaspari A,Alessandra Balduini,Massimo Abelli,Lorenzo Piemonti,Paolo Dionigi,Giuseppe Orlando,Marcello Maestri +14 more
TL;DR: Production and implantation of acellular rat renal scaffolds is a suitable platform for further studies on regenerative medicine and tissue engineering.
Journal ArticleDOI
Biocompatibility and hemocompatibility of efficiently decellularized whole porcine kidney for tissue engineering
Kamal Hany Hussein,Tarek Saleh,Ebtehal Ahmed,Ho-Hyun Kwak,Kyung-Mee Park,Se-Ran Yang,Byung-Jae Kang,Ki-Young Choi,Kyung-Sun Kang,Heung-Myong Woo +9 more
TL;DR: This work has developed a rapid decellularization technique for generating biocompatible kidney scaffolds that represents a step toward development of a transplantable organ.
Journal ArticleDOI
Bioprinting Tissue Analogues with Decellularized Extracellular Matrix Bioink for Regeneration and Tissue Models of Cartilage and Intervertebral Discs
TL;DR: The state‐of‐the‐art in cartilage and IVD dECM bioink preparation, material properties, and applications are highlighted and a vision is presented for how this field may continue to evolve in the future to empower the fabrication of scaffolds that serve as effective templates for guiding cellular assembly and organization toward the formation of functional cartilageand IVD tissues.
Journal ArticleDOI
Tissue engineering: How to build a heart
TL;DR: With thousands of people in need of heart transplants, researchers are trying to grow new organs as mentioned in this paper, but they are limited to a limited number of donor organs, such as pacemakers and pacemax.
Journal ArticleDOI
Engineering the vasculature of decellularized rat kidney scaffolds using human induced pluripotent stem cell-derived endothelial cells.
Osele Ciampi,Barbara Bonandrini,Barbara Bonandrini,Manuela Derosas,Sara Conti,Paola Rizzo,Valentina Benedetti,Marina Figliuzzi,Andrea Remuzzi,Ariela Benigni,Giuseppe Remuzzi,Giuseppe Remuzzi,Susanna Tomasoni +12 more
TL;DR: How to efficiently engineer the kidney vasculature of decellularized rat kidney scaffolds by using human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs) is reported, which was clear evidence of site-specific endothelial cell specialisation.
References
More filters
Journal ArticleDOI
Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Harald C. Ott,Thomas S Matthiesen,Saik Kia Goh,Lauren D. Black,Stefan M. Kren,Theoden I. Netoff,Doris A. Taylor +6 more
TL;DR: Eight constructs decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular architecture, competent a cellular valves and intact chamber geometry that could generate pump function in a modified working heart preparation.
Journal ArticleDOI
Tissue-engineered autologous bladders for patients needing cystoplasty
TL;DR: Engineered bladder tissues, created with autologous cells seeded on collagen-polyglycolic acid scaffolds, and wrapped in omentum after implantation, can be used in patients who need cystoplasty.
Journal ArticleDOI
Regeneration and orthotopic transplantation of a bioartificial lung
Harald C. Ott,Ben Clippinger,Claudius Conrad,Christian Schuetz,Irina Pomerantseva,Laertis Ikonomou,Darrell N. Kotton,Joseph P. Vacanti +7 more
TL;DR: Creation of a bioartificial lung requires engineering of viable lung architecture enabling ventilation, perfusion and gas exchange, and regenerated lungs into orthotopic position showed in vivo function.
Journal ArticleDOI
HLA-Mismatched Renal Transplantation without Maintenance Immunosuppression
Tatsuo Kawai,A. Benedict Cosimi,Thomas R. Spitzer,Nina Tolkoff-Rubin,Manikkam Suthanthiran,Susan L. Saidman,Juanita Shaffer,Frederic I. Preffer,Ruchuang Ding,Vijay K. Sharma,Jay A. Fishman,Bimalangshu R. Dey,Dicken S.C. Ko,Martin Hertl,Nelson Goes,Waichi Wong,Winfred W. Williams,Robert B. Colvin,Megan Sykes,David H. Sachs +19 more
TL;DR: Five patients with end-stage renal disease received combined bone marrow and kidney transplants from HLA single-haplotype mismatched living related donors, with the use of a nonmyeloablative preparative regimen, and it was possible to discontinue all immunosuppressive therapy 9 to 14 months after the transplantation.
Journal ArticleDOI
Organ engineering based on decellularized matrix scaffolds
Jeremy Song,Harald C. Ott +1 more
TL;DR: A review summarizes achievements to date and discusses the role of native ECM scaffolds in organ regeneration, which provides a promising alternative to synthetic scaffolds and a foundation for regenerative efforts.
Related Papers (5)
Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix
Basak E. Uygun,Alejandro Soto-Gutierrez,Alejandro Soto-Gutierrez,Hiroshi Yagi,Hiroshi Yagi,Maria Louisa Izamis,Maria Angela Guzzardi,Maria Angela Guzzardi,Carley Shulman,Jack M. Milwid,Naoya Kobayashi,Arno W. Tilles,Francois Berthiaume,Francois Berthiaume,Martin Hertl,Yaakov Nahmias,Yaakov Nahmias,Martin L. Yarmush,Martin L. Yarmush,Korkut Uygun +19 more