Regeneration and experimental orthotopic transplantation of a bioengineered kidney
Jeremy Song,Jacques P. Guyette,Sarah E. Gilpin,Gabriel Gonzalez,Joseph P. Vacanti,Harald C. Ott +5 more
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TLDR
To regenerate functional tissue, rat kidney scaffolds are seeded with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor, resulting in grafts that produced rudimentary urine in vitro when perfused through their intrinsic vascular bed.Abstract:
Approximately 100,000 individuals in the United States currently await kidney transplantation, and 400,000 individuals live with end-stage kidney disease requiring hemodialysis. The creation of a transplantable graft to permanently replace kidney function would address donor organ shortage and the morbidity associated with immunosuppression. Such a bioengineered graft must have the kidney's architecture and function and permit perfusion, filtration, secretion, absorption and drainage of urine. We decellularized rat, porcine and human kidneys by detergent perfusion, yielding acellular scaffolds with vascular, cortical and medullary architecture, a collecting system and ureters. To regenerate functional tissue, we seeded rat kidney scaffolds with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor. The resulting grafts produced rudimentary urine in vitro when perfused through their intrinsic vascular bed. When transplanted in an orthotopic position in rat, the grafts were perfused by the recipient's circulation and produced urine through the ureteral conduit in vivo.read more
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Cryo-chemical decellularization of the whole liver for mesenchymal stem cells-based functional hepatic tissue engineering.
Wei Cheng Jiang,Yu Hao Cheng,Meng Hua Yen,Yin Chang,Vincent W. Yang,Oscar K. Lee,Oscar K. Lee +6 more
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Decellularization and cell seeding of whole liver biologic scaffolds composed of extracellular matrix.
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Automated decellularization of intact, human-sized lungs for tissue engineering.
Andrew P. Price,Lindsay M. Godin,Alex Domek,Trevor Cotter,Jonathan D'Cunha,Doris A. Taylor,Angela Panoskaltsis-Mortari +6 more
TL;DR: An automated system that can be used to decellularize whole human-sized organs and have shown lung as an example, resulted in more consistent acellular matrix preparations as demonstrated by measuring levels of DNA, hydroxyproline (collagen), elastin, laminin, and glycosaminoglycans.
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Bioengineering Thymus Organoids to Restore Thymic Function and Induce Donor-Specific Immune Tolerance to Allografts.
Yong Fan,Asako Tajima,Saik Kia Goh,Xuehui Geng,Giulio Gualtierotti,Maria Grupillo,Antonina Coppola,Antonina Coppola,Suzanne Bertera,William A. Rudert,Ipsita Banerjee,Rita Bottino,Massimo Trucco +12 more
TL;DR: It is shown that three-dimensional scaffolds generated from decellularized mouse thymus can support thymic epithelial cell survival in culture and maintain their unique molecular properties.
References
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Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Harald C. Ott,Thomas S Matthiesen,Saik Kia Goh,Lauren D. Black,Stefan M. Kren,Theoden I. Netoff,Doris A. Taylor +6 more
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Regeneration and orthotopic transplantation of a bioartificial lung
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TL;DR: Creation of a bioartificial lung requires engineering of viable lung architecture enabling ventilation, perfusion and gas exchange, and regenerated lungs into orthotopic position showed in vivo function.
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