Regeneration and experimental orthotopic transplantation of a bioengineered kidney
Jeremy Song,Jacques P. Guyette,Sarah E. Gilpin,Gabriel Gonzalez,Joseph P. Vacanti,Harald C. Ott +5 more
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TLDR
To regenerate functional tissue, rat kidney scaffolds are seeded with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor, resulting in grafts that produced rudimentary urine in vitro when perfused through their intrinsic vascular bed.Abstract:
Approximately 100,000 individuals in the United States currently await kidney transplantation, and 400,000 individuals live with end-stage kidney disease requiring hemodialysis. The creation of a transplantable graft to permanently replace kidney function would address donor organ shortage and the morbidity associated with immunosuppression. Such a bioengineered graft must have the kidney's architecture and function and permit perfusion, filtration, secretion, absorption and drainage of urine. We decellularized rat, porcine and human kidneys by detergent perfusion, yielding acellular scaffolds with vascular, cortical and medullary architecture, a collecting system and ureters. To regenerate functional tissue, we seeded rat kidney scaffolds with epithelial and endothelial cells and perfused these cell-seeded constructs in a whole-organ bioreactor. The resulting grafts produced rudimentary urine in vitro when perfused through their intrinsic vascular bed. When transplanted in an orthotopic position in rat, the grafts were perfused by the recipient's circulation and produced urine through the ureteral conduit in vivo.read more
Citations
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Parenchymal and stromal tissue regeneration of tooth organ by pivotal signals reinstated in decellularized matrix.
Ling He,Ling He,Jian Zhou,Jian Zhou,Mo Chen,Chyuan Sheng Lin,Sahng G. Kim,Yue Zhou,Yue Zhou,Lusai Xiang,Lusai Xiang,Ming Xie,Ming Xie,Hanying Bai,Hai Yao,Changcheng Shi,Paulo G. Coelho,Timothy G. Bromage,Bin Hu,Nick Tovar,Lukasz Witek,Jia Qian Wu,Kenian Chen,Wei Gu,Jinxuan Zheng,Jinxuan Zheng,Tzong-Jen Sheu,Juan Zhong,Juan Zhong,Jin Wen,Jin Wen,Yuting Niu,Bin Cheng,Qimei Gong,Qimei Gong,David M. Owens,Milda Stanislauskas,Jasmine Pei,Gregory Chotkowski,Sainan Wang,G. W. Yang,David J. Zegarelli,Xin Shi,Myron Finkel,Wen Zhang,Wen Zhang,Junyuan Li,Jiayi Cheng,Dennis P. Tarnow,Xuedong Zhou,Zuolin Wang,Xinquan Jiang,Alexander Romanov,David W. Rowe,Songlin Wang,Ling Ye,Junqi Ling,Jeremy J. Mao +57 more
TL;DR: The Alx3 transcription factor, expressed in prenatal tooth development, is shown to revitalize adult progenitor cells in decellularized scaffolds, leading to enhanced parenchymal dental pulp and vascularized stroma regeneration in vivo.
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Dual-Purpose Bioreactors to Monitor Noninvasive Physical and Biochemical Markers of Kidney and Liver Scaffold Recellularization.
Joseph S. Uzarski,Brent M. Bijonowski,Bo Wang,Heather H. Ward,Angela Wandinger-Ness,William M. Miller,Jason A. Wertheim +6 more
TL;DR: An in-depth description and analysis of two dual-purpose perfusion bioreactors that improve upon current bioreactor designs and enable comparative analyses of ex vivo scaffold recellularization strategies and cell growth performance during long-term maintenance culture of engineered kidney or liver tissues are provided.
Journal ArticleDOI
A step towards clinical application of acellular matrix: A clue from macrophage polarization
Astgik Petrosyan,Stefano Da Sacco,Nikita Tripuraneni,Ursula Kreuser,Maria Lavarreda-Pearce,Riccardo Tamburrini,Roger E. De Filippo,Giuseppe Orlando,Paolo Cravedi,Laura Perin +9 more
TL;DR: The notion that natural ECM, even if derived from diseased kidneys promote a M2 protolerogenic macrophage polarization, is supported, thus providing novel insights on the applicability of ECM obtained from discarded organs as ideal scaffold for tissue engineering is supported.
Journal ArticleDOI
Sterilization and disinfection methods for decellularized matrix materials: Review, consideration and proposal.
Meihan Tao,Tianrang Ao,Xiaoyan Mao,Xinzhu Yan,Rabia Javed,Weijian Hou,Yang Wang,Cong Sun,Shuang Lin,Tianhao Yu,Qiang Ao,Qiang Ao,Qiang Ao +12 more
TL;DR: In this article, the authors systematically introduce and analyzes the mechanism, advantages, disadvantages, and applications of various sterilization and disinfection methods, discuss the factors influencing the selection of sterilization, and summarize the sterilization or disinfection method for various common decellularized matrix (dECM) and finally propose a graphical route for selecting an appropriate disinfection or sterilization method for dECM and a technical route for validating the selected method.
Journal ArticleDOI
Culture of iPSCs Derived Pancreatic β-Like Cells In Vitro Using Decellularized Pancreatic Scaffolds: A Preliminary Trial.
Jian Wan,Yan Huang,Pengcheng Zhou,Yibing Guo,Cen Wu,Shajun Zhu,Yao Wang,Lei Wang,Yuhua Lu,Zhiwei Wang +9 more
TL;DR: Preliminary support for regenerating insulin-secreting organs from the decellularized scaffolds combined with iPSCs derived β-like cells as a potential clinical application is provided.
References
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Journal ArticleDOI
Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Harald C. Ott,Thomas S Matthiesen,Saik Kia Goh,Lauren D. Black,Stefan M. Kren,Theoden I. Netoff,Doris A. Taylor +6 more
TL;DR: Eight constructs decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular architecture, competent a cellular valves and intact chamber geometry that could generate pump function in a modified working heart preparation.
Journal ArticleDOI
Tissue-engineered autologous bladders for patients needing cystoplasty
TL;DR: Engineered bladder tissues, created with autologous cells seeded on collagen-polyglycolic acid scaffolds, and wrapped in omentum after implantation, can be used in patients who need cystoplasty.
Journal ArticleDOI
Regeneration and orthotopic transplantation of a bioartificial lung
Harald C. Ott,Ben Clippinger,Claudius Conrad,Christian Schuetz,Irina Pomerantseva,Laertis Ikonomou,Darrell N. Kotton,Joseph P. Vacanti +7 more
TL;DR: Creation of a bioartificial lung requires engineering of viable lung architecture enabling ventilation, perfusion and gas exchange, and regenerated lungs into orthotopic position showed in vivo function.
Journal ArticleDOI
HLA-Mismatched Renal Transplantation without Maintenance Immunosuppression
Tatsuo Kawai,A. Benedict Cosimi,Thomas R. Spitzer,Nina Tolkoff-Rubin,Manikkam Suthanthiran,Susan L. Saidman,Juanita Shaffer,Frederic I. Preffer,Ruchuang Ding,Vijay K. Sharma,Jay A. Fishman,Bimalangshu R. Dey,Dicken S.C. Ko,Martin Hertl,Nelson Goes,Waichi Wong,Winfred W. Williams,Robert B. Colvin,Megan Sykes,David H. Sachs +19 more
TL;DR: Five patients with end-stage renal disease received combined bone marrow and kidney transplants from HLA single-haplotype mismatched living related donors, with the use of a nonmyeloablative preparative regimen, and it was possible to discontinue all immunosuppressive therapy 9 to 14 months after the transplantation.
Journal ArticleDOI
Organ engineering based on decellularized matrix scaffolds
Jeremy Song,Harald C. Ott +1 more
TL;DR: A review summarizes achievements to date and discusses the role of native ECM scaffolds in organ regeneration, which provides a promising alternative to synthetic scaffolds and a foundation for regenerative efforts.
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