Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser.
Christopher Kupitz,Shibom Basu,Ingo Grotjohann,Raimund Fromme,Nadia A. Zatsepin,Kimberly N. Rendek,Mark S. Hunter,Mark S. Hunter,Robert L. Shoeman,Thomas A. White,Dingjie Wang,Daniel James,Jay-How Yang,Danielle E. Cobb,Brenda Reeder,Raymond G. Sierra,Haiguang Liu,Anton Barty,Andrew Aquila,Daniel P. DePonte,Richard A. Kirian,Sadia Bari,Jesse J. Bergkamp,Kenneth R. Beyerlein,Michael J. Bogan,Carl Caleman,Tzu-Chiao Chao,Tzu-Chiao Chao,Chelsie E. Conrad,Katherine M. Davis,Holger Fleckenstein,Lorenzo Galli,Stefan P. Hau-Riege,Stephan Kassemeyer,Hartawan Laksmono,Mengning Liang,Lukas Lomb,Stefano Marchesini,Andrew V. Martin,Marc Messerschmidt,Despina Milathianaki,Karol Nass,Alexandra Ros,Shatabdi Roy-Chowdhury,Kevin Schmidt,M. Marvin Seibert,M. Marvin Seibert,Jan Steinbrener,Francesco Stellato,Lifen Yan,Chun Hong Yoon,Thomas A. Moore,Ana L. Moore,Yulia Pushkar,Garth J. Williams,Sébastien Boutet,R. Bruce Doak,Uwe Weierstall,Matthias Frank,Henry N. Chapman,John C. H. Spence,Petra Fromme +61 more
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TLDR
Time resolved experiments on PSII nano/microcrystals from Thermosynechococcus elongatus performed with the recently developed technique of serial femtosecond crystallography provide evidence that PSII undergoes significant conformational changes at the electron acceptor side and at the Mn4CaO5 core of the OEC.Abstract:
Photosynthesis, a process catalysed by plants, algae and cyanobacteria converts sunlight to energy thus sustaining all higher life on Earth. Two large membrane protein complexes, photosystem I and ...read more
Citations
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Journal ArticleDOI
Native structure of photosystem II at 1.95 Å resolution viewed by femtosecond X-ray pulses.
Michihiro Suga,Fusamichi Akita,Kunio Hirata,Go Ueno,Hironori Murakami,Yoshiki Nakajima,Tetsuya Shimizu,Keitaro Yamashita,Masaki Yamamoto,Hideo Ago,Jian Ren Shen +10 more
TL;DR: A ‘radiation-damage-free’ structure of PSII from Thermosynechococcus vulcanus in the S1 state is reported, and it is expected that this structure will provide a blueprint for the design of artificial catalysts for water oxidation.
Book
X-Rays and Extreme Ultraviolet Radiation: Principles and Applications
TL;DR: In this paper, the fundamental properties of soft x-rays and extreme ultraviolet (EUV) radiation are discussed and their applications in a wide variety of fields, including EUV lithography for semiconductor chip manufacture and soft X-ray biomicroscopy.
Journal ArticleDOI
Manganese Compounds as Water-Oxidizing Catalysts: From the Natural Water-Oxidizing Complex to Nanosized Manganese Oxide Structures
Mohammad Mahdi Najafpour,Gernot Renger,Małgorzata Hołyńska,Atefeh Nemati Moghaddam,Eva-Mari Aro,Robert Carpentier,Hiroshi Nishihara,Julian J. Eaton-Rye,Jian Ren Shen,Jian Ren Shen,Suleyman I. Allakhverdiev,Suleyman I. Allakhverdiev +11 more
TL;DR: A detailed assessment of the chemistry of Mn oxides by considering how their bulk and nanoscale properties contribute to their effectiveness as water-oxidizing catalysts and the issue of Mn complexes decomposing to Mn oxide is provided.
Journal ArticleDOI
The Structure of Photosystem II and the Mechanism of Water Oxidation in Photosynthesis
TL;DR: The overall structure of PSII is provided followed by detailed descriptions of the specific structure of the Mn4CaO5 cluster and its surrounding protein environment, based on the geometric organization revealed by the crystallographic analysis.
Journal ArticleDOI
Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL.
Michihiro Suga,Fusamichi Akita,Fusamichi Akita,Michihiro Sugahara,Minoru Kubo,Yoshiki Nakajima,Takanori Nakane,Keitaro Yamashita,Yasufumi Umena,Makoto Nakabayashi,Takahiro Yamane,Takamitsu Nakano,Mamoru Suzuki,Tetsuya Masuda,Shigeyuki Inoue,Tetsunari Kimura,Takashi Nomura,Shinichiro Yonekura,Long Jiang Yu,Tomohiro Sakamoto,Taiki Motomura,Taiki Motomura,J. Chen,J. Chen,Yuki Kato,Takumi Noguchi,Kensuke Tono,Yasumasa Joti,Takashi Kameshima,Takaki Hatsui,Eriko Nango,Rie Tanaka,Hisashi Naitow,Yoshinori Matsuura,Ayumi Yamashita,Masaki Yamamoto,Osamu Nureki,Makina Yabashi,Tetsuya Ishikawa,So Iwata,Jian Ren Shen,Jian Ren Shen,Jian Ren Shen +42 more
TL;DR: The structural changes in PSII induced by two-flash illumination at room temperature at a resolution of 2.35 Å are described, providing a mechanism for the O=O bond formation consistent with that proposed previously.
References
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Pavel V. Afonine,Ralf W. Grosse-Kunstleve,Nathaniel Echols,Jeffrey J. Headd,Nigel W. Moriarty,Marat Mustyakimov,Thomas C. Terwilliger,Alexandre Urzhumtsev,Alexandre Urzhumtsev,Peter H. Zwart,Paul D. Adams,Paul D. Adams +11 more
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Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 Å.
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TL;DR: The Linac Coherent Light Source free-electron laser has achieved coherent X-ray generation down to a wavelength of 1.2 A and at a brightness that is nearly ten orders of magnitude higher than conventional synchrotrons.
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Cooperation of charges in photosynthetic o2 evolution–i. a linear four step mechanism
TL;DR: The evolution of O2 in weak light and light flashes is studied to analyze the interactions between light induced O2 precursors and their decay in darkness and the data are compatible with a linear four step mechanism in which a trapping center successively accumulates four + charges.
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