The Possible “Proton Sponge ” Effect of Polyethylenimine (PEI) Does Not Include Change in Lysosomal pH
Rikke Vicki Benjaminsen,Maria Ahlm Mattebjerg,Jonas Rosager Henriksen,S. Moein Moghimi,Thomas Lars Andresen +4 more
Reads0
Chats0
TLDR
Measurements of lysosomal pH as a function of PEI content and correlate the results to the "proton sponge " hypothesis show that PEI does not induce change in lysoomic pH as previously suggested and quantification ofPEI concentrations inLysosomes makes it uncertain that the " proton sponge ' effect is the dominant mechanism of polyplex escape.About:
This article is published in Molecular Therapy.The article was published on 2013-01-01 and is currently open access. It has received 616 citations till now. The article focuses on the topics: Polyethylenimine.read more
Citations
More filters
Journal ArticleDOI
Using macropinocytosis for intracellular delivery of therapeutic nucleic acids to tumour cells.
TL;DR: This article reviews the delivery systems reported to be taken up by macropinocytosis and identifies new opportunities for exploiting this pathway for the intracellular delivery of nucleic acids to tumour cells.
Journal ArticleDOI
Serum-resistant, reactive oxygen species (ROS)-potentiated gene delivery in cancer cells mediated by fluorinated, diselenide-crosslinked polyplexes.
TL;DR: DSe-PEI-F showed high transfection efficiencies in cancer cells in the presence of serum, outperforming the commercial reagent PEI 25k by several orders of magnitude.
Journal ArticleDOI
Double domain polyethylenimine-based nanoparticles for integrin receptor mediated delivery of plasmid DNA.
Hossein Sadeghpour,Bahman Khalvati,Elaheh Entezar-Almahdi,Narjes Savadi,Samira Hossaini Alhashemi,Mohammad Raoufi,Ali Dehshahri +6 more
TL;DR: The results demonstrated the ability of the PEI conjugate in the formation of nanoparticles with the size of around 210 nm with higher buffering capacity and in vivo imaging of the polyplexes revealed that 99mTc-labeled PEI/plasmid DNA complexes accumulated in kidney and bladder 4 h post injection, suggesting this PEI derivative could be considered as an efficient targeted delivery system for plasmidDNA.
Journal ArticleDOI
Extracellular dsRNA: its function and mechanism of cellular uptake.
TL;DR: This review presents an overview of double-stranded RNA, addressing its roles in infection, autoimmunity, and host sensing mechanisms, with a focus on extracellular recognition and uptake by the cell.
Journal ArticleDOI
Enhancing dendritic cell activation and HIV vaccine effectiveness through nanoparticle vaccination.
TL;DR: Important advances in how ‘nanovaccinology’ can be used to develop safe and effective vaccines for HIV are summarized and the central role of dendritic cells in the immune response to vaccination is highlighted.
References
More filters
Journal ArticleDOI
A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine
Otmane Boussif,Frank Lezoualc'h,Maria Antonietta Zanta,Mojgan Mergny,Daniel Scherman,Barbara A. Demeneix,Jean-Paul Behr +6 more
TL;DR: Together, these properties make PEI a promising vector for gene therapy and an outstanding core for the design of more sophisticated devices because its efficiency relies on extensive lysosome buffering that protects DNA from nuclease degradation, and consequent lysOSomal swelling and rupture that provide an escape mechanism for the PEI/DNA particles.
Journal ArticleDOI
Understanding biophysicochemical interactions at the nano–bio interface
Andre E. Nel,Lutz Mädler,Darrell Velegol,Tian Xia,Eric M.V. Hoek,Ponisseril Somasundaran,Fred Klaessig,Vince Castranova,Mike Thompson +8 more
TL;DR: Probing the various interfaces of nanoparticle/biological interfaces allows the development of predictive relationships between structure and activity that are determined by nanomaterial properties such as size, shape, surface chemistry, roughness and surface coatings.
Journal ArticleDOI
Nonviral Vectors for Gene Delivery
TL;DR: Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.
Journal ArticleDOI
Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis.
TL;DR: The relatively high transfection efficiency of polyethylenimine vectors has been hypothesized to be due to their ability to avoid trafficking to degradative lysosomes, and according to the proton sponge hypothesis, the buffering capacity of PEI leads to osmotic swelling and rupture of endosome, resulting in the release of the vector into the cytoplasm.
Journal ArticleDOI
Chloride Accumulation and Swelling in Endosomes Enhances DNA Transfer by Polyamine-DNA Polyplexes
TL;DR: The results provide direct support for the proton sponge hypothesis and thus a rational basis for the design of improved non-viral vectors for gene delivery.