The Possible “Proton Sponge ” Effect of Polyethylenimine (PEI) Does Not Include Change in Lysosomal pH
Rikke Vicki Benjaminsen,Maria Ahlm Mattebjerg,Jonas Rosager Henriksen,S. Moein Moghimi,Thomas Lars Andresen +4 more
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TLDR
Measurements of lysosomal pH as a function of PEI content and correlate the results to the "proton sponge " hypothesis show that PEI does not induce change in lysoomic pH as previously suggested and quantification ofPEI concentrations inLysosomes makes it uncertain that the " proton sponge ' effect is the dominant mechanism of polyplex escape.About:
This article is published in Molecular Therapy.The article was published on 2013-01-01 and is currently open access. It has received 616 citations till now. The article focuses on the topics: Polyethylenimine.read more
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Squalene/polyethylenimine based non-viral vectors: synthesis and use in systems for sustained gene release
Geta David,Lilia Clima,Manuela Calin,Cristina Ana Constantinescu,Mihaela Balan-Porcarasu,Cristina M. Uritu,Bogdan C. Simionescu +6 more
TL;DR: The reported preliminary data recommend the studied biomaterials as possible candidates for the development of a new gene-activated matrix (GAM) platform based on the Sq/BPEI conjugates.
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CHARMM force field and molecular dynamics simulations of protonated polyethylenimine
Titus A. Beu,Alexandra Farcaş +1 more
TL;DR: The developed atomistic FF proves adequate for the realistic modeling of the size and protonation behavior of linear PEI, either as individual chains or composing polyplexes, as well as molecular dynamics investigations of solvated PEI.
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Surmounting tumor resistance to metallodrugs by co-loading a metal complex and siRNA in nanoparticles.
Hongzhi Qiao,Lei Zhang,Dong Fang,Zhenzhu Zhu,Weijiang He,Lihong Hu,Liuqing Di,Zijian Guo,Xiaoyong Wang +8 more
TL;DR: Adenosine-5′-triphosphate-responsive nanoparticles containing a copper complex CTND and B-cell lymphoma 2 (Bcl-2) small interfering RNA (siRNA) were constructed to cope with the resistance of cancer cells to the complex and triggered a cellular autophagy that amplified the apoptotic signals, thus revealing a novel mechanism for antagonizing the Resistance of copper complexes.
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Multifunctional polymeric micelle-based nucleic acid delivery: Current advances and future perspectives
Ivana Jarak,Miguel Pereira-Silva,Ana Cláudia Santos,Francisco Veiga,Horacio Cabral,Ana Figueiras +5 more
TL;DR: In this paper, the authors provide a critical overview of current challenges and future prospects of polymeric micelles as potential nucleic acid (NA)-based delivery systems aimed at improved NA therapies.
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Nanoscopy for endosomal escape quantification
TL;DR: The latest insights in endosomal escape of nanoparticles obtained by nanoscopy are exposed, and the features that would allow these techniques to make a great impact in the field are discussed.
References
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Journal ArticleDOI
A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine
Otmane Boussif,Frank Lezoualc'h,Maria Antonietta Zanta,Mojgan Mergny,Daniel Scherman,Barbara A. Demeneix,Jean-Paul Behr +6 more
TL;DR: Together, these properties make PEI a promising vector for gene therapy and an outstanding core for the design of more sophisticated devices because its efficiency relies on extensive lysosome buffering that protects DNA from nuclease degradation, and consequent lysOSomal swelling and rupture that provide an escape mechanism for the PEI/DNA particles.
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Understanding biophysicochemical interactions at the nano–bio interface
Andre E. Nel,Lutz Mädler,Darrell Velegol,Tian Xia,Eric M.V. Hoek,Ponisseril Somasundaran,Fred Klaessig,Vince Castranova,Mike Thompson +8 more
TL;DR: Probing the various interfaces of nanoparticle/biological interfaces allows the development of predictive relationships between structure and activity that are determined by nanomaterial properties such as size, shape, surface chemistry, roughness and surface coatings.
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Nonviral Vectors for Gene Delivery
TL;DR: Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.
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Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis.
TL;DR: The relatively high transfection efficiency of polyethylenimine vectors has been hypothesized to be due to their ability to avoid trafficking to degradative lysosomes, and according to the proton sponge hypothesis, the buffering capacity of PEI leads to osmotic swelling and rupture of endosome, resulting in the release of the vector into the cytoplasm.
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Chloride Accumulation and Swelling in Endosomes Enhances DNA Transfer by Polyamine-DNA Polyplexes
TL;DR: The results provide direct support for the proton sponge hypothesis and thus a rational basis for the design of improved non-viral vectors for gene delivery.