Journal ArticleDOI
Two distinct actin networks drive the protrusion of migrating cells
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TLDR
Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks.Abstract:
Cell migration initiates by extension of the actin cytoskeleton at the leading edge. Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks. A lamellipodium network assembled at the leading edge but completely disassembled within 1 to 3 micrometers. It was weakly coupled to the rest of the cytoskeleton and promoted the random protrusion and retraction of the leading edge. Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion.read more
Citations
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Blebbistatin modulates prostatic cell growth and contrapctility through myosin II signaling
Ping Chen,Deqiang Xu,Sheng-li Xu,He Xiao,Sheng-hong Wan,Xinghuan Wang,Michael E. DiSanto,Xinhua Zhang +7 more
TL;DR: Novel data demonstrate BLEB regulated myosin expression and functional activity and dose-dependently trigger apoptosis and retard the growth of BPH-1 and WPMY-1 cells and rat prostate tissues.
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Filament Nucleation Tunes Mechanical Memory in Active Polymer Networks
Vikrant Yadav,Deb Sankar Banerjee,Alan Tabatabai,David R. Kovar,Taeyoon Kim,Shiladitya Banerjee,Michael P. Murrell +6 more
Journal ArticleDOI
Contribution of myosin II activity to cell spreading dynamics
TL;DR: A simple analytic elastic theory of cell spreading dynamics is presented that quantitatively demonstrates how actin polymerization and myosin activity cooperate in the generation of cellular stress during spreading and demonstrates that the attenuation of myos in activity in the two regions may result in reciprocal effects on spreading.
Journal ArticleDOI
The Wdr1-LIMK-Cofilin Axis Controls B Cell Antigen Receptor-Induced Actin Remodeling and Signaling at the Immune Synapse.
Madison Bolger-Munro,Kate Choi,Faith Cheung,Yi Tian Liu,May Dang-Lawson,Nikola Deretic,Connor Keane,Michael R. Gold +7 more
TL;DR: This paper showed that WD repeat-containing protein 1 (Wdr1), LIM domain kinase (LIMK), and coactosin-like 1 (Cotl1) may also be essential for actin-dependent processes in B cells.
Journal ArticleDOI
Overview of Single-Molecule Speckle (SiMS) Microscopy and Its Electroporation-Based Version with Efficient Labeling and Improved Spatiotemporal Resolution
Sawako Yamashiro,Naoki Watanabe +1 more
TL;DR: The application of live-cell single-molecule imaging to cellular actin dynamics is described, and an electroporation-based method called eSiMS microscopy is introduced, with high efficiency, easiness and improved spatiotemporal precision.
References
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Journal ArticleDOI
Cell Migration: A Physically Integrated Molecular Process
TL;DR: The authors are grateful for financial support from the National Institutes of Health (grants GM23244 and GM53905), and to very helpful comments on the manuscript from Elliot Elson, Vlodya Gelfand, Paul Matsudaira, Julie Theriot, and Sally Zigmond.
Journal ArticleDOI
Cellular Motility Driven by Assembly and Disassembly of Actin Filaments
Thomas D. Pollard,Gary G. Borisy +1 more
TL;DR: A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematical models of the constituent reactions predict the rate of motion.
Journal ArticleDOI
The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filaments
TL;DR: It is shown that Arp2/3 complex purified from Acanthamoeba caps the pointed ends of actin filaments with high affinity and increases the critical concentration for polymerization at the pointed end from 0.6 to 1.0 microM.
Journal ArticleDOI
Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor
Aaron F. Straight,Amy Cheung,John Limouze,Irene A. Chen,Nicholas J. Westwood,James R. Sellers,Timothy J. Mitchison +6 more
TL;DR: It is shown that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis and continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.
Journal ArticleDOI
Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone.
Paul Forscher,Stephen J. Smith +1 more
TL;DR: Results suggest that actin normally polymerizes at the leading edge and then flows rearward at a rate between 3-6 microns/min, which is consistent with their being secondary to effects of CB on lamellar F-actin.
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