Journal ArticleDOI
Two distinct actin networks drive the protrusion of migrating cells
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TLDR
Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks.Abstract:
Cell migration initiates by extension of the actin cytoskeleton at the leading edge. Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks. A lamellipodium network assembled at the leading edge but completely disassembled within 1 to 3 micrometers. It was weakly coupled to the rest of the cytoskeleton and promoted the random protrusion and retraction of the leading edge. Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion.read more
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BookDOI
Advances in Systems Biology
TL;DR: This chapter discusses applications of systems biology in medicine, biotechnology and pharmaceutical industry, and computational tools: algorithms and theoretical methods for systems biology.
Journal ArticleDOI
There is More Than One Way to Model an Elephant. Experiment-Driven Modeling of the Actin Cytoskeleton
TL;DR: How comprehensive and adaptable modeling allows investigators to explain experimental observations and develop testable hypotheses on the intracellular dynamics of the actin cytoskeleton is focused on.
Journal ArticleDOI
Transient Activations of Rac1 at the Lamellipodium Tip Trigger Membrane Protrusion
Amine Mehidi,Amine Mehidi,Olivier Rossier,Olivier Rossier,Matthias Schaks,Anaël Chazeau,Anaël Chazeau,Fabien Binamé,Amanda Remorino,Mathieu Coppey,Zeynep Karatas,Zeynep Karatas,Jean-Baptiste Sibarita,Jean-Baptiste Sibarita,Klemens Rottner,Violaine Moreau,Grégory Giannone,Grégory Giannone +17 more
TL;DR: Combining single protein tracking and super-resolution imaging with loss- or gain-of-function mutants of Rho GTPases and wild-type versus mutant variants of WRC shows that selective immobilizations of activated Rac1 at the lamellipodium tip depend on effector binding, including WRC.
Journal ArticleDOI
The endosomal adaptor protein APPL1 impairs the turnover of leading edge adhesions to regulate cell migration
Joshua A. Broussard,Wan-Hsin Lin,Devi Majumdar,Bridget Anderson,Brady Eason,Claire M. Brown,Donna J. Webb +6 more
TL;DR: The adaptor protein APPL1 regulates cell migration and adhesion dynamics by inhibiting the activity of the serine/threonine kinase Akt at the cell edge and within adhesions.
Journal ArticleDOI
Nuclear actin modulates cell motility via transcriptional regulation of adhesive and cytoskeletal genes.
TL;DR: It is concluded that the level of actin in the nucleus is a transcriptional regulator for tuning keratinocyte migration and altered cytoskeletal and focal adhesion organization and inhibited cell motility.
References
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Journal ArticleDOI
Cell Migration: A Physically Integrated Molecular Process
TL;DR: The authors are grateful for financial support from the National Institutes of Health (grants GM23244 and GM53905), and to very helpful comments on the manuscript from Elliot Elson, Vlodya Gelfand, Paul Matsudaira, Julie Theriot, and Sally Zigmond.
Journal ArticleDOI
Cellular Motility Driven by Assembly and Disassembly of Actin Filaments
Thomas D. Pollard,Gary G. Borisy +1 more
TL;DR: A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematical models of the constituent reactions predict the rate of motion.
Journal ArticleDOI
The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filaments
TL;DR: It is shown that Arp2/3 complex purified from Acanthamoeba caps the pointed ends of actin filaments with high affinity and increases the critical concentration for polymerization at the pointed end from 0.6 to 1.0 microM.
Journal ArticleDOI
Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor
Aaron F. Straight,Amy Cheung,John Limouze,Irene A. Chen,Nicholas J. Westwood,James R. Sellers,Timothy J. Mitchison +6 more
TL;DR: It is shown that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis and continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.
Journal ArticleDOI
Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone.
Paul Forscher,Stephen J. Smith +1 more
TL;DR: Results suggest that actin normally polymerizes at the leading edge and then flows rearward at a rate between 3-6 microns/min, which is consistent with their being secondary to effects of CB on lamellar F-actin.
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