Journal ArticleDOI
Two distinct actin networks drive the protrusion of migrating cells
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TLDR
Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks.Abstract:
Cell migration initiates by extension of the actin cytoskeleton at the leading edge. Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks. A lamellipodium network assembled at the leading edge but completely disassembled within 1 to 3 micrometers. It was weakly coupled to the rest of the cytoskeleton and promoted the random protrusion and retraction of the leading edge. Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion.read more
Citations
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Posted ContentDOI
Side-binding proteins modulate actin filament dynamics
Alvaro H. Crevenna,Marcelino Arciniega,Aurélie Dupont,Kaja Kowalska,Oliver F. Lange,Roland Wedlich-Söldner,Don C. Lamb +6 more
TL;DR: It is shown that actin polymerization kinetics at both filament ends are strongly influenced by proteins that bind to the lateral filament surface, and that the less dynamic end, called the pointed-end, has a non-elongating state that dominates the observed filament kinetic asymmetry.
DissertationDOI
Phosphorylation of Filamin A by Cdk1/cyclin B1 Regulates Filamin A Subcellular Localization and is Important for Daughter Cell Separation
TL;DR: The objective was to validate FLNa-interactors identified from mass spectrometry from mitotic HeLa cells ............................................ 80 2.9.2 Detection of phosphorylated recombinant His-FLNa with radioactive ATP ...... 81 2.4 Regulation of Cdk1 .............................................................................................. 57 1.3.7 Actin cytoskeleton and mitotic cell rounding ................................................................ 68 Objectives ............................................................................................................................ 70.
Dissertation
Ostéoblastes et environnement physico-chimique : effets du contenu minéral matriciel et des micro-vibrations
TL;DR: In this paper, les cellules osseuses evoluent in vivo sur des matrices extracellulaires principalement formees de collagene of type I, and le degre de mineralisation varie au cours du remodelage osseux.
Journal ArticleDOI
Granger-causal inference of the lamellipodial actin regulator hierarchy by live cell imaging without perturbation
TL;DR: In this paper , a perturbation-free reconstruction of cause-effect relations among actin regulators by applying Granger-causal inference to constitutive image fluctuations that indicate regulator recruitment as a proxy for activity is presented.
References
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Journal ArticleDOI
Cell Migration: A Physically Integrated Molecular Process
TL;DR: The authors are grateful for financial support from the National Institutes of Health (grants GM23244 and GM53905), and to very helpful comments on the manuscript from Elliot Elson, Vlodya Gelfand, Paul Matsudaira, Julie Theriot, and Sally Zigmond.
Journal ArticleDOI
Cellular Motility Driven by Assembly and Disassembly of Actin Filaments
Thomas D. Pollard,Gary G. Borisy +1 more
TL;DR: A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematical models of the constituent reactions predict the rate of motion.
Journal ArticleDOI
The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filaments
TL;DR: It is shown that Arp2/3 complex purified from Acanthamoeba caps the pointed ends of actin filaments with high affinity and increases the critical concentration for polymerization at the pointed end from 0.6 to 1.0 microM.
Journal ArticleDOI
Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor
Aaron F. Straight,Amy Cheung,John Limouze,Irene A. Chen,Nicholas J. Westwood,James R. Sellers,Timothy J. Mitchison +6 more
TL;DR: It is shown that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis and continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.
Journal ArticleDOI
Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone.
Paul Forscher,Stephen J. Smith +1 more
TL;DR: Results suggest that actin normally polymerizes at the leading edge and then flows rearward at a rate between 3-6 microns/min, which is consistent with their being secondary to effects of CB on lamellar F-actin.
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