Journal ArticleDOI
Two distinct actin networks drive the protrusion of migrating cells
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TLDR
Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks.Abstract:
Cell migration initiates by extension of the actin cytoskeleton at the leading edge. Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks. A lamellipodium network assembled at the leading edge but completely disassembled within 1 to 3 micrometers. It was weakly coupled to the rest of the cytoskeleton and promoted the random protrusion and retraction of the leading edge. Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion.read more
Citations
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Journal ArticleDOI
Podosome-type adhesions and focal adhesions, so alike yet so different.
Marc R. Block,Cedric Badowski,Angélique Millon-Frémillon,Daniel Bouvard,Anne-Pascale Bouin,Eva Faurobert,Delphine Gerber-Scokaert,Emmanuelle Planus,Corinne Albiges-Rizo +8 more
TL;DR: Comparing recent advances and developments in the elucidation of the organization and dynamics of focal adhesion and podosome-type adhesions is compared in order to understand how such subcellular sites - though closely related in their composition - can be structurally and functionally different.
Book ChapterDOI
Cell migration: an overview.
TL;DR: This introduction provides a primer of the molecular and cellular insights, particularly the signaling networks, which control the migration of individual cells as well as collective migrations.
Journal ArticleDOI
Building distinct actin filament networks in a common cytoplasm.
Alphée Michelot,David G. Drubin +1 more
TL;DR: The chemical and physical mechanisms that target distinct sets of actin-binding proteins to distinct actin filament populations after nucleation are discussed, resulting in the assembly ofActin filament networks that are optimized for specific functions.
Journal ArticleDOI
Actin foci facilitate activation of the phospholipase C-γ in primary T lymphocytes via the WASP pathway
Sudha Kumari,David Depoil,David Depoil,Roberta Martinelli,Edward Judokusumo,Guillaume Carmona,Frank B. Gertler,Lance C. Kam,Christopher V. Carman,Janis K. Burkhardt,Darrell J. Irvine,Michael L. Dustin,Michael L. Dustin +12 more
TL;DR: It is concluded that WASP generates a dynamic F-actin architecture in the context of the immunological synapse, which then amplifies the downstream signals required for an optimal immune response.
Journal ArticleDOI
Structural and functional associations of apical junctions with cytoskeleton
Jun Miyoshi,Yoshimi Takai +1 more
TL;DR: Subcellular protein interactions are coordinated to induce changes in actin organization and dynamics, in response to the status of apical junctions, to create linkages that can be strong yet easily remodeled.
References
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Journal ArticleDOI
Cell Migration: A Physically Integrated Molecular Process
TL;DR: The authors are grateful for financial support from the National Institutes of Health (grants GM23244 and GM53905), and to very helpful comments on the manuscript from Elliot Elson, Vlodya Gelfand, Paul Matsudaira, Julie Theriot, and Sally Zigmond.
Journal ArticleDOI
Cellular Motility Driven by Assembly and Disassembly of Actin Filaments
Thomas D. Pollard,Gary G. Borisy +1 more
TL;DR: A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematical models of the constituent reactions predict the rate of motion.
Journal ArticleDOI
The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filaments
TL;DR: It is shown that Arp2/3 complex purified from Acanthamoeba caps the pointed ends of actin filaments with high affinity and increases the critical concentration for polymerization at the pointed end from 0.6 to 1.0 microM.
Journal ArticleDOI
Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor
Aaron F. Straight,Amy Cheung,John Limouze,Irene A. Chen,Nicholas J. Westwood,James R. Sellers,Timothy J. Mitchison +6 more
TL;DR: It is shown that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis and continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.
Journal ArticleDOI
Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone.
Paul Forscher,Stephen J. Smith +1 more
TL;DR: Results suggest that actin normally polymerizes at the leading edge and then flows rearward at a rate between 3-6 microns/min, which is consistent with their being secondary to effects of CB on lamellar F-actin.
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