Journal ArticleDOI
Two distinct actin networks drive the protrusion of migrating cells
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TLDR
Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks.Abstract:
Cell migration initiates by extension of the actin cytoskeleton at the leading edge. Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks. A lamellipodium network assembled at the leading edge but completely disassembled within 1 to 3 micrometers. It was weakly coupled to the rest of the cytoskeleton and promoted the random protrusion and retraction of the leading edge. Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion.read more
Citations
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Journal ArticleDOI
Actin stress fiber dynamics in laterally confined cells.
TL;DR: Analysis of actin cytoskeleton dynamics as a major determinant of mechanotransduction mechanisms in cells suggests that cell geometry determines actin Fiber orientation, while it also affects actin fiber transport and turnover.
Journal ArticleDOI
Effect of F-actin Organization in Lamellipodium on Viscoelasticity and Migration of Huh-7 Cells under pH Microenvironments Using AM-FM Atomic Force Microscopy
TL;DR: In this article, the authors improved the AM-FM by employing the high damping of cell culture medium to increase the signal-to-noise ratio and achieve a stable imaging of F-actin with the resolution down to 50 nm under in situ microenvironment.
Book ChapterDOI
Using Single-Protein Tracking to Study Cell Migration.
Thomas Orré,Thomas Orré,Amine Mehidi,Amine Mehidi,Sophie Massou,Sophie Massou,Olivier Rossier,Olivier Rossier,Grégory Giannone,Grégory Giannone +9 more
TL;DR: This chapter describes how single-molecule approaches have been and can be used to decipher molecular mechanisms involved in cell migration.
Journal ArticleDOI
Dynamics of leading lamellae of living fibroblasts visualized by high-speed scanning probe microscopy.
TL;DR: The combination of SPM with fluorescence microscopy showed that vinculin, a component of cell-substratum adhesion sites, localizes at the microgranules, suggesting that high-speed live-cell SPM has great potential for investigating the structural origin of cellular dynamics.
Journal ArticleDOI
α-actinin-4 drives invasiveness by regulating myosin IIB expression and myosin IIA localization.
Amlan Barai,Abhishek Mukherjee,Abhishek Mukherjee,Alakesh Das,Alakesh Das,Neha Saxena,Shamik Sen +6 more
TL;DR: In this paper, an indirect association between ACTN4 and NMM IIA mediated by a functional F-actin cytoskeleton is proposed, which is essential for retention of non-muscle myosin IIB at the cell periphery and modulation of focal adhesion dynamics.
References
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Journal ArticleDOI
Cell Migration: A Physically Integrated Molecular Process
TL;DR: The authors are grateful for financial support from the National Institutes of Health (grants GM23244 and GM53905), and to very helpful comments on the manuscript from Elliot Elson, Vlodya Gelfand, Paul Matsudaira, Julie Theriot, and Sally Zigmond.
Journal ArticleDOI
Cellular Motility Driven by Assembly and Disassembly of Actin Filaments
Thomas D. Pollard,Gary G. Borisy +1 more
TL;DR: A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematical models of the constituent reactions predict the rate of motion.
Journal ArticleDOI
The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filaments
TL;DR: It is shown that Arp2/3 complex purified from Acanthamoeba caps the pointed ends of actin filaments with high affinity and increases the critical concentration for polymerization at the pointed end from 0.6 to 1.0 microM.
Journal ArticleDOI
Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor
Aaron F. Straight,Amy Cheung,John Limouze,Irene A. Chen,Nicholas J. Westwood,James R. Sellers,Timothy J. Mitchison +6 more
TL;DR: It is shown that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis and continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.
Journal ArticleDOI
Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone.
Paul Forscher,Stephen J. Smith +1 more
TL;DR: Results suggest that actin normally polymerizes at the leading edge and then flows rearward at a rate between 3-6 microns/min, which is consistent with their being secondary to effects of CB on lamellar F-actin.
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