Journal ArticleDOI
Two distinct actin networks drive the protrusion of migrating cells
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TLDR
Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks.Abstract:
Cell migration initiates by extension of the actin cytoskeleton at the leading edge. Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks. A lamellipodium network assembled at the leading edge but completely disassembled within 1 to 3 micrometers. It was weakly coupled to the rest of the cytoskeleton and promoted the random protrusion and retraction of the leading edge. Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion.read more
Citations
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Proceedings ArticleDOI
Core-shell CdSe/ZnS Quantum dots as a dual mode spatiotemporal microscopy probe for understanding cellular responses
Philip R. LeDuc,Ying Zhang +1 more
TL;DR: The ability to use quantum dots for both modes of microscopy through a single labeling technology, which allows both dynamic and high resolution visualization with optical and electron microscopy, is demonstrated.
Posted ContentDOI
The tumor suppressor p53 promotes carcinoma invasion and collective cellular migration
Shijie He,Christopher V. Carman,Jung Hyun Lee,Bo Lan,Stephan A. Koehler,Lior Atia,Chan Young Park,Jae Hun Kim,Jennifer A. Mitchel,Jin-Ah Park,James P. Butler,Sam W. Lee,Jeffrey J. Fredberg +12 more
TL;DR: Using 2-D confluent and 3-D spheroidal cell motility assays with bladder carcinoma cells and colorectal carcinomas cells, it is reported that loss of p53 can decrease cell motilities and invasion.
Role and Regulation of Myosin IIA in Natural Killer Cell Cytotoxicity
TL;DR: A novel role for nonmuscle myosin IIA is presented in NK cells, where myos in IIA directly associates with lytic granules and guides their transport across actin filaments to facilitate secretion of their contents.
Book ChapterDOI
Single-Protein Tracking to Study Protein Interactions During Integrin-Based Migration
A V Radhakrishnan,A V Radhakrishnan,Tianchi Chen,Tianchi Chen,Jose Filipe Nunes Vicente,Jose Filipe Nunes Vicente,Thomas Orré,Thomas Orré,Amine Mehidi,Amine Mehidi,Amine Mehidi,Olivier Rossier,Olivier Rossier,Grégory Giannone,Grégory Giannone +14 more
TL;DR: This chapter describes how recent developments in superresolution microscopy (SRM) and single-protein tracking (SPT) start to unravel protein interactions and actions in subcellular molecular assemblies driving cell migration.
References
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Journal ArticleDOI
Cell Migration: A Physically Integrated Molecular Process
TL;DR: The authors are grateful for financial support from the National Institutes of Health (grants GM23244 and GM53905), and to very helpful comments on the manuscript from Elliot Elson, Vlodya Gelfand, Paul Matsudaira, Julie Theriot, and Sally Zigmond.
Journal ArticleDOI
Cellular Motility Driven by Assembly and Disassembly of Actin Filaments
Thomas D. Pollard,Gary G. Borisy +1 more
TL;DR: A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematical models of the constituent reactions predict the rate of motion.
Journal ArticleDOI
The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filaments
TL;DR: It is shown that Arp2/3 complex purified from Acanthamoeba caps the pointed ends of actin filaments with high affinity and increases the critical concentration for polymerization at the pointed end from 0.6 to 1.0 microM.
Journal ArticleDOI
Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor
Aaron F. Straight,Amy Cheung,John Limouze,Irene A. Chen,Nicholas J. Westwood,James R. Sellers,Timothy J. Mitchison +6 more
TL;DR: It is shown that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis and continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.
Journal ArticleDOI
Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone.
Paul Forscher,Stephen J. Smith +1 more
TL;DR: Results suggest that actin normally polymerizes at the leading edge and then flows rearward at a rate between 3-6 microns/min, which is consistent with their being secondary to effects of CB on lamellar F-actin.
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