Journal ArticleDOI
Two distinct actin networks drive the protrusion of migrating cells
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Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks.Abstract:
Cell migration initiates by extension of the actin cytoskeleton at the leading edge. Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks. A lamellipodium network assembled at the leading edge but completely disassembled within 1 to 3 micrometers. It was weakly coupled to the rest of the cytoskeleton and promoted the random protrusion and retraction of the leading edge. Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion.read more
Citations
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Journal ArticleDOI
Unraveling the determinants of protrusion formation.
TL;DR: The results show that FC maturation/cytoskeletal integration affects factor 5, because FC elongation/integration was correlated with its values, and conclude that cells establish functional domains by rearranging the cytoskeleton.
Dissertation
Electric signals regulated immunomodulation and wound healing
TL;DR: It is proposed that the EF-induced re-distribution of the receptors on the cell surface results in a shift of membrane receptors between the cathode-facing and the anodes-facing membrane of the cell, which would help to overcome the threshold of signal transduction at the higher density receptor side.
Journal ArticleDOI
Fully coupled numerical model of actin treadmilling in the lamellipodium of the cell.
TL;DR: A highly detailed theoretical model of the actin treadmilling process is adopted and a coupled unsteady finite element formulation is developed, which reveals a promising applicability of classical finite element methods to derive an in silico testing platform for the actIn treadmills processes in motile cells, which could allow for an extension to other biophysical effects.
Journal ArticleDOI
Cytoskeletal interactions with the outside world.
TL;DR: A recent Keystone meeting on cell migration and adhesion showcased advances in the field and called for further research into intracellular cytoskeletal responses to cell motility.
Journal ArticleDOI
Proteomes, kinases and signalling pathways in virus-induced filopodia, as potential antiviral therapeutics targets.
Isah Abubakar Aliyu,Abdulhadi Sale Kumurya,Jamilu Abubakar Bala,Jamilu Abubakar Bala,H Yahaya,H Yahaya,Hayatu Saidu +6 more
TL;DR: This review will describe some reported virus‐host protein interactions on filopodia with the aim of identifying potential new anti‐virus therapeutic targets.
References
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Journal ArticleDOI
Cell Migration: A Physically Integrated Molecular Process
TL;DR: The authors are grateful for financial support from the National Institutes of Health (grants GM23244 and GM53905), and to very helpful comments on the manuscript from Elliot Elson, Vlodya Gelfand, Paul Matsudaira, Julie Theriot, and Sally Zigmond.
Journal ArticleDOI
Cellular Motility Driven by Assembly and Disassembly of Actin Filaments
Thomas D. Pollard,Gary G. Borisy +1 more
TL;DR: A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematical models of the constituent reactions predict the rate of motion.
Journal ArticleDOI
The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filaments
TL;DR: It is shown that Arp2/3 complex purified from Acanthamoeba caps the pointed ends of actin filaments with high affinity and increases the critical concentration for polymerization at the pointed end from 0.6 to 1.0 microM.
Journal ArticleDOI
Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor
Aaron F. Straight,Amy Cheung,John Limouze,Irene A. Chen,Nicholas J. Westwood,James R. Sellers,Timothy J. Mitchison +6 more
TL;DR: It is shown that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis and continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.
Journal ArticleDOI
Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone.
Paul Forscher,Stephen J. Smith +1 more
TL;DR: Results suggest that actin normally polymerizes at the leading edge and then flows rearward at a rate between 3-6 microns/min, which is consistent with their being secondary to effects of CB on lamellar F-actin.
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