Journal ArticleDOI
Two distinct actin networks drive the protrusion of migrating cells
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TLDR
Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks.Abstract:
Cell migration initiates by extension of the actin cytoskeleton at the leading edge. Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks. A lamellipodium network assembled at the leading edge but completely disassembled within 1 to 3 micrometers. It was weakly coupled to the rest of the cytoskeleton and promoted the random protrusion and retraction of the leading edge. Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion.read more
Citations
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Journal ArticleDOI
Wnt/PCP Signaling Contribution to Carcinoma Collective Cell Migration and Metastasis.
Kacey VanderVorst,Courtney A. Dreyer,Sara E. Konopelski,Hyun Min Lee,Hsin-Yi Henry Ho,Kermit L. Carraway +5 more
TL;DR: Key studies are summarized that address the mechanisms by which Wnt/PCP signaling mediate collective cell migration in developmental and tumor contexts and discuss how component asymmetry may govern the spatiotemporal control of downstream cytoskeletal effectors to promote collective cell motility.
Journal ArticleDOI
Cells as liquid motors: mechanosensitivity emerges from collective dynamics of actomyosin cortex.
Jocelyn Etienne,Jonathan Fouchard,Démosthène Mitrossilis,Nathalie Bufi,Pauline Durand-Smet,Atef Asnacios +5 more
TL;DR: Using a minimal model of the dynamics of actomyosin cortex, it is shown that the interplay of myosin power strokes with the rapidly remodeling actin network results in a regulation of force and cell shape that adapts to the stiffness of the environment.
Journal ArticleDOI
The nucleus of endothelial cell as a sensor of blood flow direction
Eugene Tkachenko,Edgar Gutierrez,Semion K. Saikin,Per Fogelstrand,Chungho Kim,Alex Groisman,Mark H. Ginsberg +6 more
TL;DR: Data indicate that ECs can sense the direction and strength of blood flow through the hydrodynamic drag applied to their nuclei, indicating that an intact acto-myosin cytoskeleton resists flow-induced polarization.
Journal ArticleDOI
A composition-dependent molecular clutch between T cell signaling condensates and actin
Jonathon A. Ditlev,Jonathon A. Ditlev,Anthony R. Vega,Darius Vasco Köster,Darius Vasco Köster,Xiaolei Su,Xiaolei Su,Tomomi Tani,Ashley M. Lakoduk,Ronald D. Vale,Ronald D. Vale,Satyajit Mayor,Satyajit Mayor,Khuloud Jaqaman,Michael K. Rosen,Michael K. Rosen +15 more
TL;DR: The data show that Nck and WASP form a clutch between LAT condensates and actin in vitro and suggest that compositional changes may enable condensate movement by distinct actin networks in different regions of the IS.
Journal ArticleDOI
Actin Filament Elasticity and Retrograde Flow Shape the Force-Velocity Relation of Motile Cells
Juliane Zimmermann,Claudia Brunner,Mihaela Enculescu,Michael Goegler,Allen J. Ehrlicher,Josef A. Käs,Martin Falcke +6 more
TL;DR: This work presents a mechanism quantitatively explaining the cell's force-velocity relation and its changes upon application of drugs that hinder actin polymerization or actomyosin-based contractility.
References
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Journal ArticleDOI
Cell Migration: A Physically Integrated Molecular Process
TL;DR: The authors are grateful for financial support from the National Institutes of Health (grants GM23244 and GM53905), and to very helpful comments on the manuscript from Elliot Elson, Vlodya Gelfand, Paul Matsudaira, Julie Theriot, and Sally Zigmond.
Journal ArticleDOI
Cellular Motility Driven by Assembly and Disassembly of Actin Filaments
Thomas D. Pollard,Gary G. Borisy +1 more
TL;DR: A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematical models of the constituent reactions predict the rate of motion.
Journal ArticleDOI
The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filaments
TL;DR: It is shown that Arp2/3 complex purified from Acanthamoeba caps the pointed ends of actin filaments with high affinity and increases the critical concentration for polymerization at the pointed end from 0.6 to 1.0 microM.
Journal ArticleDOI
Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor
Aaron F. Straight,Amy Cheung,John Limouze,Irene A. Chen,Nicholas J. Westwood,James R. Sellers,Timothy J. Mitchison +6 more
TL;DR: It is shown that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis and continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.
Journal ArticleDOI
Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone.
Paul Forscher,Stephen J. Smith +1 more
TL;DR: Results suggest that actin normally polymerizes at the leading edge and then flows rearward at a rate between 3-6 microns/min, which is consistent with their being secondary to effects of CB on lamellar F-actin.
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