Journal ArticleDOI
Two genetically separable steps in the differentiation of thymic epithelium.
Michael Nehls,Bruno Kyewski,Martin Messerle,Ralph Waldschütz,Kerstin Schüddekopf,Andrew J.H. Smith,Thomas Boehm +6 more
Reads0
Chats0
TLDR
The WHN transcription factor was shown to be the product of the nude locus and these results define the first genetically separable steps during thymic epithelial differentiation.Abstract:
The development of the thymus depends initially on epithelial-mesenchymal and subsequently on reciprocal lympho-stromal interactions. The genetic steps governing development and differentiation of the thymic microenvironment are unknown. With the use of a targeted disruption of the whn gene, which recapitulates the phenotype of the athymic nude mouse, the WHN transcription factor was shown to be the product of the nude locus. Formation of the thymic epithelial primordium before the entry of lymphocyte progenitors did not require the activity of WHN. However, subsequent differentiation of primitive precursor cells into subcapsular, cortical, and medullary epithelial cells of the postnatal thymus did depend on activity of the whn gene. These results define the first genetically separable steps during thymic epithelial differentiation.read more
Citations
More filters
Journal ArticleDOI
Edg-1, the G protein–coupled receptor for sphingosine-1-phosphate, is essential for vascular maturation
Yujing Liu,Ryuichi Wada,Tadashi Yamashita,Yide Mi,Chu-Xia Deng,John P. Hobson,Hans M. Rosenfeldt,Victor E. Nava,Sung Suk Chae,Menq Jer Lee,Catherine H. Liu,Timothy Hla,Sarah Spiegel,Richard L. Proia +13 more
TL;DR: The data reveal Edg-1 to be the first G protein-coupled receptor required for blood vessel formation and show that sphingolipid signaling is essential during mammalian development.
Journal ArticleDOI
Promiscuous gene expression in medullary thymic epithelial cells mirrors the peripheral self.
TL;DR: It is found that this promiscuous gene expression was a cell-autonomous property of medullary epithelial cells and was maintained during the entire period of thymic T cell output, which may facilitate tolerance induction to self-antigens that would otherwise be temporally or spatially secluded from the immune system.
Journal ArticleDOI
Forkhead transcription factors: Key players in development and metabolism
Peter Carlsson,Margit Mahlapuu +1 more
TL;DR: Forkhead proteins are not among the largest transcription factor families, but display a remarkable functional diversity and are involved in a wide variety of biological processes.
Journal ArticleDOI
p63 Is Essential for the Proliferative Potential of Stem Cells in Stratified Epithelia
TL;DR: P63, a gene whose deletion in mice results in the catastrophic loss of all stratified epithelia, is analyzed and it is demonstrated that p63 is strongly expressed in epithelial cells with high clonogenic and proliferative capacity and that stem cells lacking p63 undergo a premature proliferative rundown.
Journal ArticleDOI
Altered nociception, analgesia and aggression in mice lacking the receptor for substance P.
Carmen De Felipe,Juan Herrero,John A. O'Brien,JA Palmer,Christopher A. Doyle,Andrew J.H. Smith,Jennifer M.A. Laird,Carlos Belmonte,Fernando Cervero,SP Hunt +9 more
TL;DR: Although substance P did not mediate the signalling of acute pain or hyperalgesia, it was essential for the full development of stress-induced analgesia and for an aggressive response to territorial challenge, demonstrating that the peptide plays an unexpected role in the adaptive response to stress.
References
More filters
Journal Article
Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors.
TL;DR: The properties and uses of a monoclonal antibody, H57-597, produced from hamsters, which reacts with surface receptors on all alpha beta TCR-bearing cells and does not react with receptors on gamma delta+ T cells.
Journal ArticleDOI
New member of the winged-helix protein family disrupted in mouse and rat nude mutations
TL;DR: The whn gene, designated whn, encodes a new member of the winged-helix domain family of transcription factors and is disrupted on mouse nu and rat rnuN alleles, the first member of this class of genes to be implicated in a specific developmental defect in vertebrates.
Journal ArticleDOI
The oncogenic cysteine-rich lim domain protein rbtn2 is essential for erythroid development
Alan J. Warren,William H. Colledge,Mark B. L. Carlton,Martin J. Evans,Andrew J.H. Smith,Terence H. Rabbitts +5 more
TL;DR: In vitro differentiation of yolk sac tissue from homozygous mutant mice and sequentially targeted double-mutant ES cells demonstrates a block to erythroid development, which shows a pivotal role for a LIM domain protein in lineage specification during mammalian development and suggests that RBTN2 and GATA-1 are critical at similar stages of erystroid differentiation.
Journal ArticleDOI
Positive selection of thymocytes.
TL;DR: Recent work suggests that the peptide/MHC ligand for positive selection may bind with low avidity to the TCR, which could have implications for the nature of T cell recognition during positive selection.
Journal ArticleDOI
Perinatal lethality and defects in hindbrain development in mice homozygous for a targeted mutation of the zinc finger gene Krox20.
Pamela J. Swiatek,T Gridley +1 more
TL;DR: It is shown that Krox20 plays an essential role during development of the hindbrain and associated cranial sensory ganglia in mice and fusions resulted in a disorganization of the nerve roots of these ganglia as they entered the brain stem.