Journal ArticleDOI
Visualizing PU.1 activity during hematopoiesis.
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TLDR
Examination of GFP+ vs GFP- populations shows that differentiation, but not commitment, to the myeloid lineage requires PU.1.1, suggesting that PU.About:
This article is published in Experimental Hematology.The article was published on 2005-04-01. It has received 100 citations till now. The article focuses on the topics: Cellular differentiation & Green fluorescent protein.read more
Citations
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Journal ArticleDOI
Transcription factors in myeloid development: balancing differentiation with transformation.
Frank Rosenbauer,Daniel G. Tenen +1 more
TL;DR: Transcription factors have been shown to be key determinants in the orchestration of myeloid identity and differentiation fates, and therapies designed to restore defective transcription factor functions are an attractive option in the treatment ofMyeloid and other human cancers.
Journal ArticleDOI
The Transcriptional Regulation of B Cell Lineage Commitment
Stephen L. Nutt,Barbara L. Kee +1 more
TL;DR: This review focuses on recent studies that have revealed that instead of a simple transcriptional hierarchy, efficient B cell commitment and differentiation requires the combinatorial activity of multiple transcription factors in a complex gene regulatory network.
Journal ArticleDOI
M-CSF instructs myeloid lineage fate in single haematopoietic stem cells
Noushine Mossadegh-Keller,Sandrine Sarrazin,Sandrine Sarrazin,Sandrine Sarrazin,Prashanth K. Kandalla,Prashanth K. Kandalla,Prashanth K. Kandalla,Leon Espinosa,E. Richard Stanley,Stephen L. Nutt,Jordan Moore,Michael H. Sieweke +11 more
TL;DR: It is shown that macrophage colony-stimulating factor (M-CSF, also called CSF1), a myeloid cytokine released during infection and inflammation, can directly induce the myeloids master regulator PU.1 and instruct myeloidal cell-fate change in mouse HSCs, independently of selective survival or proliferation.
Journal ArticleDOI
Reciprocal Activation of GATA-1 and PU.1 Marks Initial Specification of Hematopoietic Stem Cells into Myeloerythroid and Myelolymphoid Lineages
Yojiro Arinobu,Shinichi Mizuno,Yong Chong,Hirokazu Shigematsu,Tadafumi Iino,Tadafumi Iino,Hiromi Iwasaki,Thomas Graf,Robin Mayfield,Susan Chan,Philippe Kastner,Koichi Akashi,Koichi Akashi +12 more
TL;DR: The reciprocal activation of GATA-1 and PU.1 primarily organizes the hematopoietic lineage fate decision to form the earliest hematopolietic branchpoint that comprises isolatable myeloerythroid and myelolymphoid progenitor populations.
Journal ArticleDOI
The Transcription Factor PU.1 Controls Dendritic Cell Development and Flt3 Cytokine Receptor Expression in a Dose-Dependent Manner
Sebastian Carotta,Aleksandar Dakic,Angela D'Amico,Swee Heng Milon Pang,Swee Heng Milon Pang,Kylie T. Greig,Kylie T. Greig,Stephen L. Nutt,Li Wu +8 more
TL;DR: Molecular studies demonstrate that PU.1 directly regulated Flt3 in a concentration-dependent manner, as Sfpi1(+/-) cells displayed reduced expression of FlT3 and impaired DC formation, identifying PU.
References
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Journal ArticleDOI
A clonogenic common myeloid progenitor that gives rise to all myeloid lineages
TL;DR: The prospective identification, purification and characterization, using cell-surface markers and flow cytometry, of a complementary clonogenic common myeloid progenitor that gives rise to all myeloids lineages is reported.
Journal ArticleDOI
Identification of Clonogenic Common Lymphoid Progenitors in Mouse Bone Marrow
TL;DR: The Lin(-)IL-7R(+)Thy-1(-)Sca-1loc-Kit(lo) population from adult mouse bone marrow possessed a rapid lymphoid-restricted (T, B, and NK) reconstitution capacity in vivo but completely lacked myeloid differentiation potential either in vivo or in vitro.
Journal ArticleDOI
Multilineage gene expression precedes commitment in the hemopoietic system
Ming Hu,Diane S. Krause,Mel Greaves,Saul J. Sharkis,Michael Dexter,Clare M Heyworth,Tariq Enver +6 more
TL;DR: Using single cell RT-PCR, it is shown that erythroid and myeloid gene expression programs can be initiated by the same cell prior to exclusive commitment to the erythyroid or granulocytic lineages.
Journal ArticleDOI
Regulation of B lymphocyte and macrophage development by graded expression of PU.1
Rodney P. DeKoter,Harinder Singh +1 more
TL;DR: Using retroviral transduction of PU.1 complementary DNA into mutant hematopoietic progenitors, it is demonstrated that differing concentrations of the protein regulate the development of B lymphocytes as compared with macrophages.
Journal ArticleDOI
Thymopoiesis independent of common lymphoid progenitors
David Allman,Arivazhagan Sambandam,Sungjune Kim,Sungjune Kim,Juli P. Miller,Antonio J. Pagán,David Well,Anita Meraz,Avinash Bhandoola +8 more
TL;DR: It is concluded that ETPs can develop via a CLP-independent pathway, and analysis of Ikaros-deficient mice that exhibit ongoing thymopoiesis without B lymphopoeisis revealed near-normal frequencies of thymic E TPs, yet undetectable numbers of BM CLPs.
Related Papers (5)
Regulation of B lymphocyte and macrophage development by graded expression of PU.1
Rodney P. DeKoter,Harinder Singh +1 more