Chalk River Laboratories

About: Chalk River Laboratories is a based out in . It is known for research contribution in the topics: Neutron diffraction & Neutron scattering. The organization has 2297 authors who have published 2700 publications receiving 73287 citations.

Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.

Abstract: Epidemiological studies indicate long-term risks of ionizing radiation on the heart, even at moderate doses. In this study, we investigated the inflammatory, thrombotic and fibrotic late responses of the heart after low-dose irradiation (IR) with specific emphasize on the dose rate. Hypercholesterolemic ApoE-deficient mice were sacrificed 3 and 6 months after total body irradiation (TBI) with 0.025, 0.05, 0.1, 0.5 or 2 Gy at low (1 mGy/min) or high dose rate (150 mGy/min). The expression of inflammatory and thrombotic markers was quantified in frozen heart sections (CD31, E-selectin, thrombomodulin, ICAM-1, VCAM-1, collagen IV, Thy-1, and CD45) and in plasma samples (IL6, KC, MCP-1, TNFα, INFγ, IL-1β, TGFβ, INFγ, IL-10, sICAM-1, sE-selectin, sVCAM-1 and fibrinogen) by fluorescence analysis and ELISA. We found that even very low irradiation doses induced adaptive late responses, such as increases of capillary density and changes in collagen IV and Thy-1 levels indicating compensatory regulation. Slight decreases of ICAM-1 levels and reduction of Thy 1 expression at 0.025–0.5 Gy indicate anti-inflammatory effects, whereas at the highest dose (2 Gy) increased VCAM-1 levels on the endocardium may represent a switch to a pro-inflammatory response. Plasma samples partially confirmed this pattern, showing a decrease of proinflammatory markers (sVCAM, sICAM) at 0.025–2.0 Gy. In contrast, an enhancement of MCP-1, TNFα and fibrinogen at 0.05–2.0 Gy indicated a proinflammatory and prothrombotic systemic response. Multivariate analysis also revealed significant age-dependent increases (KC, MCP-1, fibrinogen) and decreases (sICAM, sVCAM, sE-selectin) of plasma markers. This paper represents local and systemic effects of low-dose irradiation, including also age- and dose rate-dependent responses in the ApoE-/- mouse model. These insights in the multiple inflammatory/thrombotic effects caused by low-dose irradiation might facilitate an individual evaluation and intervention of radiation related, long-term side effects but also give important implications for low dose anti-inflammatory radiotherapy.

Phase transition in solid acetonitrile

Abstract: The structure of the low temperature phase of acetonitrile was determined from neutron powder diffraction data. The lattice parameters were measured as a function of temperature and the structure of the high temperature phase, as previously determined, was confirmed. At 12 K, the structure is orthorhombic, Cmc21, a = 5·9895(3) A, b = 5·2079(2) A, c = 7·7317(4) A. A transformation to a monoclinic phase, P21/c, a = 4·1182(7) A, b = 8·2865(10) A, c = 7·9946(11) A, β = 100·4(6)°, takes places between 220 and 229 K. Equilibrium structures for the two phases were calculated using simple model potentials and the calculated structures are very similar to those observed. The nature of the phase transition was investigated by means of these model potentials.

Hierarchical, self-similar structure in native squid pen.

Abstract: The structure of native squid pen (gladius) was investigated in two different species on different length scales. By combining microscopy, atomic force microscopy (AFM), and X-ray diffraction, the experiments probed length scales from millimetres down to nanometres. The gladii showed a hierarchical, self-similar structure in the optical experiments with fibres of different size oriented along the long axis of the gladius. The fibre-like structure was reproduced at the nanoscale in AFM measurements and fibres with diameters of 500 μm, 100 μm, 10 μm, 2 μm and 0.2 μm were observed. Their molecular structure was determined using X-ray diffraction. In the squid gladius, the chitin molecules are known to form nano-crystallites of monoclinic lattice symmetry wrapped in a protein layer, resulting in β-chitin nano-fibrils. Signals corresponding to the α-coil protein phase and β-chitin crystallites were observed in the X-ray experiments and their orientation with respect to the fibre-axis was determined. The size of a nano-fibril was estimated from the X-ray experiments to be about 150 × 300 A. About 100 of these nano-fibrils are needed to form a 0.2 μm thick micro-fibre. We found that the molecular structure is highly anisotropic with ∼90% of the α-coils and β-chitin crystallites oriented along the fibre-axis, indicating a strong correlation between the macroscale structure and molecular orientation.

Fast prediction of thermal distortion in metal powder bed fusion additive manufacturing: Part 2, a quasi-static thermo-mechanical model

Abstract: The additive manufacturing (AM) process metal powder bed fusion (PBF) can quickly produce complex parts with mechanical properties comparable to that of wrought materials. However, thermal stress accumulated during Metal PBF may induce part distortion and even cause failure of the entire process. This manuscript is the second part of two companion manuscripts that collectively present a part-scale simulation method for fast prediction of thermal distortion in Metal PBF. The first part provides a fast prediction of the temperature history in the part via a thermal circuit network (TCN) model. This second part uses the temperature history from the TCN to inform a model of thermal distortion using a quasi-static thermo-mechanical model (QTM). The QTM model distinguished two periods of Metal PBF, the thermal loading period and the stress relaxation period. In the thermal loading period, the layer-by-layer build cycles of Metal PBF are simulated, and the thermal stress accumulated in the build process is predicted. In the stress relaxation period, the removal of parts from the substrate is simulated, and the off-substrate part distortion and residual stress are predicted. Validation of part distortion predicted by the QTM model against both experiment and data in literature showed a relative error less than 20%. This QTM, together with the TCN, offers a framework for rapid, part-scale simulations of Metal PBF that can be used to optimize the build process and parameters.