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Showing papers by "Chonbuk National University published in 2005"


Journal ArticleDOI
TL;DR: The crystallite size was decreased to constant value for Cell 2 treated at >or= 15 wt% NaOH, and the crystalliteSize of Cell 2-C (cellulose II) was smaller than that of Cell 1 ( cellulose I) treated at 5-10 wt%, and the CI(XD) was calculated by the method of Jayme and Knolle.

1,113 citations


Journal ArticleDOI
TL;DR: The observation that soluble UA alters the proliferation/migration and NO release of human vascular cells, mediated by the expression of CRP, calls for careful reconsideration of the role of UA in hypertension and vascular disease.
Abstract: Recent experimental and human studies have shown that hyperuricemia is associated with hypertension, systemic inflammation, and cardiovascular disease mediated by endothelial dysfunction and pathologic vascular remodeling Elevated levels of C-reactive protein (CRP) have emerged as one of the most powerful independent predictors of cardiovascular disease In addition to being a marker of inflammation, recent evidence suggests that CRP may participate directly in the development of atherosclerotic vascular disease For investigating whether uric acid (UA)-induced inflammatory reaction and vascular remodeling is related to CRP, the UA-induced expression of CRP in human vascular smooth muscle cells (HVSMC) and human umbilical vein endothelial cells (HUVEC) was examined, as well as the pathogenetic role of CRP in vascular remodeling It is interesting that HVSMC and HUVEC expressed CRP mRNA and protein constitutively, revealing that vascular cells are another source of CRP production UA (6 to 12 mg/dl) upregulated CRP mRNA expression in HVSMC and HUVEC with a concomitant increase in CRP release into cell culture media Inhibition of p38 or extracellular signal-regulated kinase 44/42 significantly suppressed UA-induced CRP expression, implicating these pathways in the response to UA UA stimulated HVSMC proliferation whereas UA inhibited serum-induced proliferation of HUVEC assessed by 3H-thymidine uptake and cell counting, which was attenuated by co-incubation with probenecid, the organic anion transport inhibitor, suggesting that entry of UA into cells is responsible for CRP expression UA also increased HVSMC migration and inhibited HUVEC migration In HUVEC, UA reduced nitric oxide (NO) release Treatment of vascular cells with anti-CRP antibody revealed a reversal of the effect of UA on cell proliferation and migration in HVSMC and NO release in HUVEC, which suggests that CRP expression may be responsible for UA-induced vascular remodeling This is the first study to show that soluble UA, at physiologic concentrations, has profound effects on human vascular cells The observation that UA alters the proliferation/migration and NO release of human vascular cells, mediated by the expression of CRP, calls for careful reconsideration of the role of UA in hypertension and vascular disease

805 citations


Journal ArticleDOI
TL;DR: Results indicated that the main mechanism of Cr(VI) removal was a redox reaction between Cr( VI) and the dead fungal biomass, which is quite different from previously reported mechanisms.

398 citations


Journal ArticleDOI
TL;DR: Findings indicated that the amino and carboxyl groups take part in the Cr(VI) removal from the aqueous phase.

384 citations


Journal ArticleDOI
TL;DR: Woven fabrics, consisting of porous filaments via electrospinning, may be suitable candidates as tissue engineering scaffolds, according to the feasibility of three-dimensional fabric as scaffold matrices evaluated.
Abstract: Electric field-driven fiber formation (electrospinning) is developing into a practical means for preparing novel porous filament with unusual structures and affordable mechanical properties. Polycaprolactone (PCL) was dissolved in solvent mixtures of methylene chloride/N,N-dimethyl formamide with ratios of 100/0, 75/25, and 50/50 (v/v) for electrospinning. The filament was formed by coagulation of the spinning solution following the well-known principle of phase separation in polymer solutions valid in other wet shaping processes. A strand of electrospun porous filament consisted of fibers ranging from 0.5 to 12 microm in diameter. To evaluate the feasibility of three-dimensional fabric as scaffold matrices, the plain weave, which is the simplest of the weaves and the most common, was prepared with porous PCL filament. The growth characteristics of MCF-7 mammary carcinoma cells in the woven fabrics showed the important role of matrix microstructure in proliferation. This study has shown that woven fabrics, consisting of porous filaments via electrospinning, may be suitable candidates as tissue engineering scaffolds.

279 citations


Journal ArticleDOI
Juan Antonio Aguilar-Saavedra1, Ahmed Ali, Benjamin C. Allanach2, Richard L. Arnowitt3, Howard Baer4, Jonathan Bagger5, Csaba Balázs6, Vernon Barger7, Michael Barnett8, A. Bartl9, Marco Battaglia8, Philip Bechtle10, Geneviève Bélanger, Alexander Belyaev11, Edmond L. Berger6, G.A. Blair12, Edouard Boos13, Marcela Carena14, S.Y. Choi15, Frank F. Deppisch, A. De Roeck16, Klaus Desch17, Marco Aurelio Diaz18, Abdelhak Djouadi19, Bhaskar Dutta3, S. Dutta20, S. Dutta10, Helmut Eberl21, John Ellis16, Jens Erler22, H. Fraas23, Ayres Freitas24, T. Fritzsche25, Rohini M. Godbole26, G. Gounaris27, Jaume Guasch28, John F. Gunion29, Naoyuki Haba30, Howard E. Haber31, K. Hagiwara, Liyuan Han32, Tao Han7, Hong-Jian He33, Sven Heinemeyer16, S. Hesselbach34, Keisho Hidaka35, I. Hinchliffe8, Martin Hirsch36, K. Hohenwarter-Sodek9, Wolfgang Hollik25, W. S. Hou37, Tobias Hurth16, Tobias Hurth10, I. Jack38, Yi Jiang32, D.R.T. Jones38, J. Kalinowski39, T. Kamon3, Gordon L. Kane40, Sin Kyu Kang41, Thomas Kernreiter9, Wolfgang Kilian, Choong Sun Kim42, Stephen F. King43, O. Kittel44, Michael Klasen, J. L. Kneur45, K. Kovarik21, Michael Kramer46, Sabine Kraml16, Remi Lafaye47, Paul Langacker48, Heather E. Logan49, W. G. Ma32, W. Majerotto21, H. U. Martyn46, Konstantin Matchev50, David J. Miller51, Myriam Mondragón22, Gudrid Moortgat-Pick16, Stefano Moretti43, Takehiko Mori52, Gilbert Moultaka45, Steve Muanza53, M. M. Mühlleitner, Biswarup Mukhopadhyaya54, U. Nauenberg55, Mihoko M. Nojiri56, D. Nomura11, H. Nowak, N. Okada, Keith A. Olive57, W. Oller21, Michael E. Peskin10, Tilman Plehn25, Giacomo Polesello, Werner Porod36, Werner Porod24, Fernando Quevedo2, David L. Rainwater58, Jürgen Reuter, Peter J. Richardson59, Krzysztof Rolbiecki39, Probir Roy60, Reinhold Rückl23, Heidi Rzehak61, P. Schleper62, Kim Siyeon63, Peter Skands14, P. Slavich, Dominik Stöckinger59, Paraskevas Sphicas16, Michael Spira61, Tim M. P. Tait6, Daniel Tovey64, José W. F. Valle36, Carlos E. M. Wagner65, Carlos E. M. Wagner6, Ch. Weber21, Georg Weiglein59, Peter Wienemann17, Z.-Z. Xing, Y. Yamada66, Jin Min Yang, D. Zerwas19, P.M. Zerwas, Ren-You Zhang32, X. Zhang, S.-H. Zhu67 
University of Lisbon1, University of Cambridge2, Texas A&M University3, Florida State University4, Johns Hopkins University5, Argonne National Laboratory6, University of Wisconsin-Madison7, Lawrence Berkeley National Laboratory8, University of Vienna9, Stanford University10, Michigan State University11, Royal Holloway, University of London12, Moscow State University13, Fermilab14, Chonbuk National University15, CERN16, University of Freiburg17, Pontifical Catholic University of Chile18, University of Paris19, University of Delhi20, Austrian Academy of Sciences21, National Autonomous University of Mexico22, University of Würzburg23, University of Zurich24, Max Planck Society25, Indian Institute of Science26, Aristotle University of Thessaloniki27, University of Barcelona28, University of California, Davis29, University of Tokushima30, University of California, Santa Cruz31, University of Science and Technology of China32, Tsinghua University33, Uppsala University34, Tokyo Gakugei University35, Spanish National Research Council36, National Taiwan University37, University of Liverpool38, University of Warsaw39, University of Michigan40, Seoul National University41, Yonsei University42, University of Southampton43, University of Bonn44, University of Montpellier45, RWTH Aachen University46, Laboratoire d'Annecy-le-Vieux de physique des particules47, University of Pennsylvania48, Carleton University49, University of Florida50, University of Glasgow51, University of Tokyo52, University of Lyon53, Harish-Chandra Research Institute54, University of Colorado Boulder55, Kyoto University56, University of Minnesota57, University of Rochester58, Durham University59, Tata Institute of Fundamental Research60, Paul Scherrer Institute61, University of Hamburg62, Chung-Ang University63, University of Sheffield64, University of Chicago65, Tohoku University66, Peking University67
TL;DR: In this article, a supersymmetry Parameter Analysis SPA (SPA) scheme is proposed based on a consistent set of conventions and input parameters, which connect parameters in different schemes and relate the Lagrangian parameters to physical observables at LHC and high energy e+e-linear collider experiments.
Abstract: High-precision analyses of supersymmetry parameters aim at reconstructing the fundamental supersymmetric theory and its breaking mechanism. A well defined theoretical framework is needed when higher-order corrections are included. We propose such a scheme, Supersymmetry Parameter Analysis SPA, based on a consistent set of conventions and input parameters. A repository for computer programs is provided which connect parameters in different schemes and relate the Lagrangian parameters to physical observables at LHC and high energy e+e- linear collider experiments, i.e., masses, mixings, decay widths and production cross sections for supersymmetric particles. In addition, programs for calculating high-precision low energy observables, the density of cold dark matter (CDM) in the universe as well as the cross sections for CDM search experiments are included. The SPA scheme still requires extended efforts on both the theoretical and experimental side before data can be evaluated in the future at the level of the desired precision. We take here an initial step of testing the SPA scheme by applying the techniques involved to a specific supersymmetry reference point.

249 citations


Journal ArticleDOI
TL;DR: In this article, a method to design low-pass filters (LPF) having a defected ground structure (DGS) and broadened transmission-line elements is proposed, which can be applied in design N-pole LPFs for N/spl les/5.
Abstract: A method to design low-pass filters (LPF) having a defected ground structure (DGS) and broadened transmission-line elements is proposed. The previously presented technique for obtaining a three-stage LPF using DGS by Lim et al. is generalized to propose a method that can be applied in design N-pole LPFs for N/spl les/5. As an example, a five-pole LPF having a DGS is designed and measured. Accurate curve-fitting results and the successive design process to determine the required size of the DGS corresponding to the LPF prototype elements are described. The proposed LPF having a DGS, called a DGS-LPF, includes transmission-line elements with very low impedance instead of open stubs in realizing the required shunt capacitance. Therefore, open stubs, teeor cross-junction elements, and high-impedance line sections are not required for the proposed LPF, while they all have been essential in conventional LPFs. Due to the widely broadened transmission-line elements, the size of the DGS-LPF is compact.

237 citations


Journal ArticleDOI
TL;DR: In this article, a disc-type electrode was introduced to reduce the taper of the machining depth and 3D micro structures including a hemisphere with 60 µm diameter were fabricated by electrochemical milling.
Abstract: In this paper, electrochemical machining (ECM) for fabricating micro structures is presented. By applying ultra short pulses, dissolution of a workpiece can be restricted to the region very close to an electrode. Using this method, 3D micro structures were machined on stainless steel. Good surface quality of the structures was obtained in the low concentration electrolyte, 0.1 M H2SO4. In ECM, when the machining depth increases, structures taper. To reduce the taper, a disc-type electrode is introduced. By electrochemical milling, various 3D micro structures including a hemisphere with 60 µm diameter were fabricated.

234 citations


Journal ArticleDOI
TL;DR: In this paper, the hydrogen storage capacities of palladium-and vanadium-doped carbon nanotubes (CNTs) at room temperature were investigated using the Sieverts apparatus.

213 citations


Journal ArticleDOI
TL;DR: Azadirachtin was the most potent in all experiments and produced almost 100% larval mortality at 1 ppm concentration, and first to third larval instars were more susceptible to the neem limonoids.

204 citations


Journal ArticleDOI
TL;DR: In this article, the removal of hexavalent chromium from aqueous solution was carried out in batch experiments using dead biomass of four fungal strains (Aspergillus niger, Rhizopus oryzae, Saccharomyces cerevisiae and Penicillium chrysogenum).

Journal ArticleDOI
TL;DR: The formation of high-density ZnO nanorods has been achieved by a vapor-solid growth mechanism using metallic zinc powder and oxygen gas as source materials for zinc and oxygen, respectively as discussed by the authors.

Journal ArticleDOI
TL;DR: Bibong metabasites have internal isochron ages at 225-258 Ma and the major elements of the metabasites display an island arc affinity as mentioned in this paper, and the petrochemical and geochronological data suggest the southwestern part of the Gyeonggi massif as an extension of the Triassic Dabie-Sulu collision belt in China.
Abstract: Metabasite lenses occur within granitic gneiss at Bibong in the southwestern part of the Gyeonggi massif, South Korea. Bibong metabasites experienced an initial eclogite facies metamorphism (17.0–20.9 kbar, 835°–860°C), which was succeeded by a granulite facies metamorphism (11.5–14.6 kbar, 830°–850°C) and finally overprinted by an amphibolite facies metamorphism (6.7–11.0 kbar, 570°–740°C). The metabasites have Sm‐Nd internal isochron ages at 225–258 Ma, and the major elements of the metabasites display an island arc affinity. The petrochemical and geochronological data suggest the southwestern part of the Gyeonggi massif as an extension of the Triassic Dabie‐Sulu collision belt in China.

Journal ArticleDOI
TL;DR: In this paper, polyethylene (PE)/clay nanocomposites were synthesized by melt-intercalation using either PP-g-MA or PE-g -MA as a compatibilizer.

Journal ArticleDOI
TL;DR: Food consumption, efficiency of conversion of ingested and digested food, relative growth rate, and consumption index values declined significantly, but at the same time an increase in approximate digestibility was also observed.

Journal ArticleDOI
TL;DR: Results indicate neem limonoids affects gut enzyme activities of rice leaffolder larvae, and these effects are most pronounced in early instars.

Journal ArticleDOI
TL;DR: In vapour phase toxicity tests with female lice, benzaldehyde and salicylaldehyde were much more effective in closed containers than in open ones, indicating that the mode of delivery of these compounds was largely due to action in the vapours phase.

Journal ArticleDOI
TL;DR: In this article, the aerodynamic performance prediction of a unique 30kW counter-rotating (C/R) wind turbine system, which consists of the main rotor and the auxiliary rotor, has been investigated by using the quasi-steady strip theory.

Journal ArticleDOI
TL;DR: A protective role of PPARγ is demonstrated in the pathogenesis of the asthma phenotype through regulation of PTEN expression throughregulation of phosphatase and tensin homologue deleted on chromosome ten.
Abstract: The ligand-activated nuclear receptor peroxisome proliferator-activated receptor gamma (PPARgamma) has been shown to regulate cell activation, differentiation, proliferation, and/or apoptosis. PPARgamma is also associated with anti-inflammatory responses. However, the signaling mechanism remains elusive. We have used a mouse model for asthma to determine the effect of PPARgamma agonists, rosiglitazone or pioglitazone, and PPARgamma on allergen-induced bronchial inflammation and airway hyperresponsiveness. Administration of PPARgamma agonists or adenovirus carrying PPARgamma cDNA (AdPPARgamma) reduced bronchial inflammation and airway hyperresponsiveness. Expression of PPARgamma was increased by ovalbumin (OVA) inhalation, and the increase was further enhanced by the administration of the PPARgamma agonists or AdPPARgamma. Levels of IL-4, IL-5, IL-13, and eosinophil cationic protein were increased after OVA inhalation, and the increased levels were significantly reduced by the administration of PPARgamma agonists or AdPPARgamma. The results also showed that the administration of PPARgamma agonists or AdPPARgamma up-regulated phosphatase and tensin homologue deleted on chromosome ten (PTEN) expression in allergen-induced asthmatic lungs. This up-regulation correlated with decreased phosphatidylinositol 3-kinase activity as measured by reduced phosphorylation of Akt. These findings demonstrate a protective role of PPARgamma in the pathogenesis of the asthma phenotype through regulation of PTEN expression.

Journal ArticleDOI
TL;DR: In this paper, the effect of operating parameters such as initial pH, supporting electrolyte concentration, phenol concentration, and charge input was studied using Box-Behnken second order composite experimental design.
Abstract: Electrochemical oxidation of phenol using a Ti/TiO2-RuO2-IrO2 anode in the presence of chloride as the supporting electrolyte was investigated. The experiments were performed in an undivided batch reactor. Preliminary investigations showed that only a small fraction of phenol was oxidized by direct electrolysis, while complete degradation of phenol was achieved by indirect electrochemical oxidation using chloride as a supporting electrolyte. The effect of operating parameters such as initial pH, supporting electrolyte concentration, phenol concentration, and charge input was studied using Box-Behnken second order composite experimental design. The effect of current density on COD removal was studied separately. TOC removal and AOX formation were studied for selected conditions. It was found that the formation of chlorinated organic compounds was pronounced at the beginning of electrolysis, but it was reduced to lower levels by extended electrolysis.

Journal ArticleDOI
03 Jan 2005-Gene
TL;DR: The present results indicate that the Oslti6 genes are part of a battery of cold stress defense-related genes regulated by a common switch.

Journal ArticleDOI
TL;DR: It is found that the ST depends on the type of intermodular connections, and the exponent associated with the order parameter of the ST is found to be beta approximately 1 different from beta(MF) approximately 1/2 obtained from the scale-free network with the same degree distribution but the absence of modular structure.
Abstract: We study the synchronization transition (ST) of a modified Kuramoto model on two different types of modular complex networks. It is found that the ST depends on the type of intermodular connections. For the network with decentralized (centralized) intermodular connections, the ST occurs at finite coupling constant (behaves abnormally). Such distinct features are found in the yeast protein interaction network and the Internet, respectively. Moreover, by applying the finite-size scaling analysis to an artificial network with decentralized intermodular connections, we obtain the exponent associated with the order parameter of the ST to be beta approximately 1 different from beta(MF) approximately 1/2 obtained from the scale-free network with the same degree distribution but the absence of modular structure, corresponding to the mean field value.

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TL;DR: In this paper, a model for vapor-solid (VS) growth mechanism of ZnO nanowires was presented, and the as-grown nanostructures are highly crystalline in nature and preferably grown along the [0, 0,0,1] direction.

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TL;DR: The combined effect of the three biopesticides resulted in a considerable decrease in nutritional indices of the rice leaffolder, indicating strong deterrence.
Abstract: Laboratory assays were done to evaluate the effect of neem seed kernel extract (Azadirachta indica A. Juss),Vitex negundo L. (Lamiales: Verbenaceae) leaf extract, andBacillus thuringiensis (Berliner), applied separately or together, on nutritional indices of the rice leaffolderCnaphalocrocis medinalis (Guenee) (Lepidoptera: Pyralidae). All three biopesticides suppressed feeding and larval growth and low concentrations affected the larval performance. The combined effect of the three biopesticides resulted in a considerable decrease in nutritional indices, indicating strong deterrence. Dose response relationships were established with respect to frass production and larval growth. The efficiency of conversion of ingested and digested food was considerably reduced.

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TL;DR: Most of the genes identified in this study were not previously believed or known to play a role in the pathogenesis of SG infection in chickens, and should contribute to a better understanding of the pathogenic mechanisms involved in progression of disease caused by SG.
Abstract: Salmonella gallinarum (SG) is a non-motile host-adapted salmonella that causes fowl typhoid, a severe systemic disease responsible for significant economic losses to the poultry industry worldwide. This study describes the application of a PCR-based signature-tagged mutagenesis system to identify in vivo-essential genes of SG. Ninety-six pools representing 1152 SG mutants were screened in a natural-host chicken infection model. Twenty presumptive attenuated mutants were identified and examined further. The identity of the disrupted gene in each mutant was determined by cloning of the DNA sequences adjacent to the transposon, followed by sequencing and comparison with the bacterial genome database. In vitro and in vivo competition indices were determined for each identified mutant and a total of 18 unique, attenuating gene disruptions were identified. These mutations represented six broad genomic classes: Salmonella pathogenicity island-1 (SPI-1), SPI-2, SPI-10, SPI-13, SPI-14 and non-SPI-encoded virulence genes. SPI-13 and SPI-14 are newly identified and designated in this study. Most of the genes identified in this study were not previously believed or known to play a role in the pathogenesis of SG infection in chickens. Each STM identified mutant showed competitiveness and/or virulence defects, confirmed by in vitro and in vivo assays, and challenge tests. This study should contribute to a better understanding of the pathogenic mechanisms involved in progression of disease caused by SG, and identification of novel live vaccine candidates and new potential antibiotic targets.

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TL;DR: The essential oils of A. scoparia and A. capillaris exhibited considerable inhibitory effects against all oral bacteria tested, while their major components demonstrated various degrees of growth inhibition.
Abstract: The chemical composition of the essential oils obtained from Artemisia scoparia Waldst. et Kitamura and Artemisia capillaris Thunb. was analyzed by GC/MS. The essential oil of A. scoparia was rich in camphor (11.0 %), 1,8-cineole (21.5 %), and beta-caryophyllene (6.8 %) as the major compounds, whereas A. capillaris oil was rich in beta-pinene (9.4 %), beta-caryophyllene (11.1 %), and capillene (32.7 %). The essential oils and some of their major compounds were tested for their antimicrobial activity against 15 different genera of oral bacteria. The essential oils of A. scoparia and A. capillaris exhibited considerable inhibitory effects against all oral bacteria tested, while their major components demonstrated various degrees of growth inhibition.

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TL;DR: A significant proportion of IPMTs are malignant, although the overall prognosis of IPMT is superior to that of ordinary pancreatic cancer, and radical surgery is recommended for IPMT with the predictors of malignancy.
Abstract: Despite recently increasing numbers of reports on intraductal papillary mucinous tumors (IPMTs), difficulties still remain in terms of diagnosis, treatment, and prognosis. The purpose of this multicenter study was to evaluate the clinicopathologic features of IPMT in Korea and to suggest predictive criteria for malignancy in IPMT. We retrospectively reviewed the clinicopathologic data of 208 patients who underwent operations for IPMT between 1993 and 2002 at 28 institutes in Korea. Of the 208 patients (mean age, 61 years), 147 were men and 61 were women. A total of 124 patients underwent pancreatoduodenectomy, 42 underwent distal pancreatectomy, 17 underwent total pancreatectomy, and 25 underwent limited pancreatic resection. There were 128 benign cases (adenoma, n = 62; borderline, n = 66) and 80 malignant cases (noninvasive, n = 29; invasive, n = 51). A significant difference in 5-year survival was observed between the benign and malignant groups (92.6% vs. 65.3%; P = .006). Of the six factors (age, location, duct dilatation, mural nodule, main duct type, and tumor size) that showed statistical differences by univariate analysis between the benign and malignant groups, three were significant by multivariate analysis—namely, mural nodule (P = .009), tumor size (P = .023), and a dilated duct size (P = .010). A significant proportion of IPMTs are malignant, although the overall prognosis of IPMT is superior to that of ordinary pancreatic cancer. Radical surgery is recommended for IPMT with the predictors of malignancy: mural nodule, tumor size (⩾30 mm), and dilated duct size (⩾12 mm).

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TL;DR: Comparison of metal concentrations in roadside sediments with mean background values in sediments collected from first- or second-order streams in Korea shows that Zn, Cu and Pb are most affected by anthropogenic inputs.

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TL;DR: Obesity was associated with poor cognition, and obesity indexes must be carefully considered to reveal this relationship.
Abstract: This study aimed to investigate associations between obesity and poor cognitive performance using data from a community study of 467 individuals aged > or = 65 years in South Korea. Cognitive function was ascertained using the Korean Mini-Mental State Examination (K-MMSE), and obesity using anthropometric measures including waist circumference and body mass index (BMI). Poor cognitive performance was present in 37% of the sample. General obesity (BMI > or = 25) and poor cognition were strongly associated in the presence of abdominal obesity. Poor cognition was negatively associated with overweight (BMI 23-25) with normal waist circumference. Interaction terms with abdominal obesity for BMI increase were significant (p = 0.007). Obesity was associated with poor cognition, and obesity indexes must be carefully considered to reveal this relationship.

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TL;DR: It is demonstrated that 17beta-estradiol (E2) up-regulates PI3K in an ERalpha-dependent manner, but not ERbeta, and stimulates cell growth in breast cancer cells, suggesting that estrogen activates PI3k/Akt signaling through ER alpha-dependent mechanism in MCF-7 cells.