Kyungpook National University

About: Kyungpook National University is a education organization based out in Daegu, South Korea. It is known for research contribution in the topics: Population & Catalysis. The organization has 20497 authors who have published 42107 publications receiving 834608 citations.

Nanoparticle-enhanced surface plasmon resonance detection of proteins at attomolar concentrations: comparing different nanoparticle shapes and sizes.

Abstract: The application of biofunctionalized nanoparticles possessing various shapes and sizes for the enhanced surface plasmon resonance (SPR) detection of a protein biomarker at attomolar concentrations is described. Three different gold nanoparticle shapes (cubic cages, rods and quasi-spherical) with each possessing at least one dimension in the 40–50 nm range were systematically compared. Each nanoparticle (NP) was covalently functionalized with an antibody (anti-thrombin) and used as part of a sandwich assay in conjunction with a Au SPR chip modified with a DNA-aptamer probe specific to thrombin. The concentration of each NP-antibody conjugate solution was first optimized prior to establishing that the quasi-spherical nanoparticles resulted in the greatest enhancement in sensitivity with the detection of thrombin at concentrations as low as 1 aM. When nanorod and nanocage antibody conjugates were instead used, the minimum target concentrations detected were 10 aM (rods) and 1 fM (cages). This is a significan...

Anti‐obese property of fucoxanthin is partly mediated by altering lipid‐regulating enzymes and uncoupling proteins of visceral adipose tissue in mice

Abstract: This study investigated the anti-obesity effects of fucoxanthin in diet-induced obesity mice fed a high-fat diet (20% fat, wt/wt). The mice were supplemented with two doses of fucoxanthin (0.05 and 0.2%, wt/wt) for 6 wk. Fucoxanthin significantly lowered body weight and visceral fat-pads weights compared with the control group without altering food intake. In epididymal adipose tissue of fucoxanthin-fed mice, adipocyte sizes and mRNA expression of lipogenic and fatty acid beta-oxidation enzymes were significantly altered in a dose-dependent manner. Plasma leptin level was significantly lower in the fucoxanthin groups than in the control group, while the adiponectin level was elevated. Fucoxanthin significantly down-regulated various lipogenic enzyme activities in epididymal adipose tissue with a simultaneous decrease in fatty acid beta-oxidation activity. The 0.2% fucoxanthin supplement led to increase mRNA expression of uncoupling protein-1 (UCP-1) and UCP-3 in brown adipose tissue and that of UCP-2 in the epididymal white adipose tissue. However, the 0.05% fucoxanthin only elevated UCP-1 mRNA expression in epididymal white adipose tissue. These results suggest that the anti-obesity effect of fucoxanthin could be mediated by altering lipid-regulating enzymes and UCPs in the visceral fat tissues and plasma adipokine levels.

Acinetobacter baumannii outer membrane protein A targets the nucleus and induces cytotoxicity.

Abstract: Acinetobacter baumannii is an emerging opportunistic pathogen responsible for healthcare-associated infections. The outer membrane protein A of A. baumannii (AbOmpA) is the most abundant surface protein that has been associated with the apoptosis of epithelial cells through mitochondrial targeting. The nuclear translocation of AbOmpA and the subsequent pathology on host cells were further investigated. AbOmpA directly binds to eukaryotic cells. AbOmpA translocates to the nucleus by a novel monopartite nuclear localization signal (NLS). The introduction of rAbOmpA into the cells or a transient expression of AbOmpA-EGFP causes the nuclear localization of these proteins, while the fusion proteins of AbOmpADeltaNLS-EGFP and AbOmpA with substitutions in residues lysine to alanine in the NLS sequences represent an exclusively cytoplasmic distribution. The nuclear translocation of AbOmpA induces cell death in vitro. Furthermore, the microinjection of rAbOmpA into the nucleus of Xenopus laevis embryos fails to develop normal embryogenesis, thus leading to embryonic death. We propose a novel pathogenic mechanism of A. baumannii regarding the nuclear targeting of the bacterial structural protein AbOmpA.

AKM-IoV: Authenticated Key Management Protocol in Fog Computing-Based Internet of Vehicles Deployment

Abstract: Internet of Vehicles (IoV) is an intelligent application of Internet of Things (IoT) in smart transportation that takes intelligent commitments to the passengers to improve traffic safety and efficiency, and generate a more enjoyable driving and riding environment. Fog cloud-based IoV is another variant of mobile cloud computing where vehicular cloud and Internet can co-operate in more effective way in IoV. However, more increasing dependence on wireless communication, control, and computing technology makes IoV more dangerous to prospective attacks. For secure communication among vehicles, road-side units, fog and cloud servers, we design a secure authenticated key management protocol in fog computing-based IoV deployment, called AKM-IoV. In the designed AKM-IoV, after mutual authentication between communicating entities in IoV they establish session keys for secure communications. AKM-IoV is tested for its security analysis using the formal security analysis under the widely accepted real-or-random (ROR) model, informal, and formal security verification using the broadly accepted automated validation of Internet security protocols and applications (AVISPAs) tool. The practical demonstration of AKM-IoV is shown using the NS2 simulation. In addition, a detailed comparative study is conducted to show the efficiency and functionality and security features supported by AKM-IoV as compared to other existing recent protocols.