A single cell characterisation of human embryogenesis identifies pluripotency transitions and putative anterior hypoblast centre.
Matteo A. Molè,Matteo A. Molè,Tim H. H. Coorens,Marta N. Shahbazi,Marta N. Shahbazi,Antonia Weberling,Bailey A. T. Weatherbee,Carlos W. Gantner,Carmen Sancho-Serra,Lucy Richardson,Abbie Drinkwater,Najma Syed,Stephanie Engley,Philip Snell,Leila Christie,Kay Elder,Alison Campbell,Simon Fishel,Simon Fishel,Sam Behjati,Sam Behjati,Roser Vento-Tormo,Magdalena Zernicka-Goetz,Magdalena Zernicka-Goetz +23 more
TLDR
In this article, the authors identify a group of asymmetrically positioned extra-embryonic hypoblast cells expressing inhibitors of BMP, NODAL and WNT signalling pathways and suggest that these cells can act as the anterior singalling centre to pattern the epiblast.Abstract:
Following implantation, the human embryo undergoes major morphogenetic transformations that establish the future body plan. While the molecular events underpinning this process are established in mice, they remain unknown in humans. Here we characterise key events of human embryo morphogenesis, in the period between implantation and gastrulation, using single-cell analyses and functional studies. First, the embryonic epiblast cells transition through different pluripotent states and act as a source of FGF signals that ensure proliferation of both embryonic and extra-embryonic tissues. In a subset of embryos, we identify a group of asymmetrically positioned extra-embryonic hypoblast cells expressing inhibitors of BMP, NODAL and WNT signalling pathways. We suggest that this group of cells can act as the anterior singalling centre to pattern the epiblast. These results provide insights into pluripotency state transitions, the role of FGF signalling and the specification of anterior-posterior axis during human embryo development.read more
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Single-cell transcriptomic characterization of a gastrulating human embryo.
Richard C. V. Tyser,Elmir Mahammadov,Shota Nakanoh,Ludovic Vallier,Antonio Scialdone,Shankar Srinivas +5 more
TL;DR: In this paper, the authors characterized the single-cell transcriptional profiles of an entire gastrulating human embryo, staged to be between 16 and 19 days after fertilization, and identified primordial germ cells, red blood cells and various mesodermal and endodermal cell types.
Journal ArticleDOI
Early human embryonic development: Blastocyst formation to gastrulation.
Janet Rossant,Patrick P.L. Tam +1 more
TL;DR: Rossant and Tam as mentioned in this paper discuss new insights into human embryogenesis gathered from successes in culturing human embryos in vitro and stem-cell-based embryo models, and outline what questions still need answering.
Journal ArticleDOI
Spatial profiling of early primate gastrulation in utero
Sophie Bergmann,Christopher A. Penfold,Erin Slatery,Dylan Siriwardena,Charis Drummer,Stephen J. Clark,Stanley E. Strawbridge,Keiko Kishimoto,Alice Vickers,Mukul Tewary,Timo N Kohler,Florian Hollfelder,Wolf Reik,Erika Sasaki,Rüdiger Behr,Thorsten Boroviak +15 more
TL;DR: In this article , spatial transcriptomics and stem-cell-based embryo models were used to reveal the molecular code of lineage specification in the primate embryo and provide an in vivo reference to decipher human development.
Journal ArticleDOI
The exploration of pluripotency space: Charting cell state transitions in peri-implantation development.
TL;DR: In this article, the authors examined these recent findings in light of our knowledge of peri-implantation mouse and human development, and considered the implications of this work for deriving human embryo models from pluripotent cells.
Journal ArticleDOI
In vitro attachment and symmetry breaking of a human embryo model assembled from primed embryonic stem cells.
TL;DR: In this article , a pre-formed polarized epithelial epiblast and extra-embryonic cells are assembled into a structure that mimics the dish-attached human embryo, and the model attaches in vitro and, in the absence of exogenous morphogens, breaks anteroposterior symmetry.
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