Assessing sample and miRNA profile quality in serum and plasma or other biofluids
Thorarinn Blondal,Søren Jensby Nielsen,Adam Baker,Ditte Andreasen,Peter Mouritzen,Maria Wrang Teilum,Ina K. Dahlsveen +6 more
TLDR
Normal reference ranges for circulating miRNAs in serum and plasma as well as a hemolysis indicator based on microRNA expression are developed.About:
This article is published in Methods.The article was published on 2013-01-01 and is currently open access. It has received 567 citations till now.read more
Citations
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Plasma phospholipids identify antecedent memory impairment in older adults
Mark Mapstone,Amrita K. Cheema,Massimo S. Fiandaca,Xiaogang Zhong,Timothy R. Mhyre,Linda MacArthur,William J. Hall,Susan G. Fisher,Derick R. Peterson,James M Haley,Michael Nazar,Steven A. Rich,Dan J Berlau,Carrie B. Peltz,Ming Tan,Claudia H. Kawas,Howard J. Federoff +16 more
TL;DR: This work discovered and validated a set of ten lipids from peripheral blood that predicted phenoconversion to either amnestic mild cognitive impairment or Alzheimer's disease within a 2–3 year timeframe with over 90% accuracy and may be sensitive to early neurodegeneration of preclinical Alzheimer’s disease.
MicroRNAs: Target Recognition and Regulatory Functions
TL;DR: In this article, a review outlines the current understanding of miRNA target recognition in animals and discusses the widespread impact of miRNAs on both the expression and evolution of protein-coding genes.
Journal ArticleDOI
MicroRNA regulatory networks in human adipose tissue and obesity
Peter Arner,Agné Kulyté +1 more
TL;DR: Given their role in regulating transcriptional networks, miRNAs in adipose tissue might offer tangible targets for treating metabolic disorders.
Journal ArticleDOI
The Impact of Hemolysis on Cell-Free microRNA Biomarkers.
Michaela B. Kirschner,J. James B. Edelman,J. James B. Edelman,Steven Kao,Michael P. Vallely,Michael P. Vallely,Nico van Zandwijk,Glen Reid +7 more
TL;DR: The microRNA content of hemolyzed and non-hemolyzed plasma as well as RBCs was profiled to obtain a profile of microRNAs in the circulation affected or unaffected by hemolysis, highlighting the importance of rigorous quality control of plasma/serum samples used for measurement of circulating micro RNAs.
Journal ArticleDOI
MicroRNAs in metabolism
TL;DR: This review emphasizes current ideas and controversies within miRNA research in metabolism, and emphasizes their importance as both endocrine signalling molecules and potentially disease markers.
References
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Journal ArticleDOI
MicroRNAs: Target Recognition and Regulatory Functions
TL;DR: The current understanding of miRNA target recognition in animals is outlined and the widespread impact of miRNAs on both the expression and evolution of protein-coding genes is discussed.
Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TL;DR: It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).
Journal ArticleDOI
Circulating microRNAs as stable blood-based markers for cancer detection
Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more
TL;DR: It is shown here that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity and established the measurement of tumor-derived mi RNAs in serum or plasma as an important approach for the blood-based detection of human cancer.
Journal ArticleDOI
The widespread regulation of microRNA biogenesis, function and decay.
TL;DR: This work has shown that the regulation of miRNA metabolism and function by a range of mechanisms involving numerous protein–protein and protein–RNA interactions has an important role in the context-specific functions of miRNAs.
Journal ArticleDOI
Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data.
TL;DR: It is shown that the first approach can lead to PCR efficiencies that vary over a 0.2 range, whereas the second approach may be off by 0.26, and proposed linear regression on the Log(fluorescence) per cycle number data as an assumption-free method to calculate starting concentrations of mRNAs and PCRefficiencies for each sample.
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