Journal ArticleDOI
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TLDR
It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.Abstract:
Recent studies have demonstrated that transplanted bone marrow cells can turn into unexpected lineages including myocytes, hepatocytes, neurons and many others. A potential problem, however, is that reports discussing such 'transdifferentiation' in vivo tend to conclude donor origin of transdifferentiated cells on the basis of the existence of donor-specific genes such as Y-chromosome markers. Here we demonstrate that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3. Moreover, spontaneously fused bone marrow cells can subsequently adopt the phenotype of the recipient cells, which, without detailed genetic analysis, might be interpreted as 'dedifferentiation' or transdifferentiation.read more
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Bone marrow-derived B cells preserve ventricular function after acute myocardial infarction.
Traci T. Goodchild,Keith A. Robinson,Wenxin Pang,Fernando Tondato,Jianhua Cui,Johnail Arrington,Lisa Godwin,Mark Ungs,Nadia Carlesso,Nadine Weich,Mark C. Poznansky,Nicolas A. Chronos +11 more
TL;DR: Intramyocardial injection of B cells into early post-ischemic myocardium preserved cardiac function by cardiomyocyte salvage, and other BM MNC subtypes were either ineffective or suppressed cardioprotection conferred by an enriched B cell population.
Journal ArticleDOI
Genetic programs and responses of neural stem/progenitor cells during demyelination: potential insights into repair mechanisms in multiple sclerosis
TL;DR: Recent progress in elucidating the genetic programs and dynamics of NSC and OPC proliferation, differentiation, and apoptosis, including the response to demyelination are reviewed.
Journal ArticleDOI
Lack of additional benefit of intracoronary transplantation of autologous peripheral blood stem cell in patients with acute myocardial infarction.
Jin-Ho Choi,Jin-Oh Choi,Wang-Soo Lee,Il Rhee,Sang-Chol Lee,Hyeon-Cheol Gwon,Sang Hoon Lee,Yeon Hyeon Choe,Dae-Won Kim,Wonhee Suh,Duk-Kyung Kim,Eun-Seok Jeon +11 more
TL;DR: Additional intracoronary infusion of PBSC was safe and feasible for the patients with AMI who had undergone percutaneous coronary intervention, but did not lead to a significant improvement in LV function compared to standard reperfusion treatment.
Journal ArticleDOI
Model systems and experimental conditions that lead to effective repopulation of the liver by transplanted cells.
David A. Shafritz,Michael Oertel +1 more
TL;DR: The purpose of this review is to present the major systems that have been used for liver repopulating, the variables involved in obtaining successful repopulation and what has been achieved in these various systems to date with different cell types.
Journal ArticleDOI
Epigenetic modifiers promote efficient generation of neural-like cells from bone marrow-derived mesenchymal cells grown in neural environment.
TL;DR: Using pharmacological agents that interfere with the main components of the epigenetic program such as DNA methylation and histone deacetylation, high‐level expression of embryonic and neural stem cell marker Sox2 is induced in bone marrow‐derived mesenchymal stem cells (MSCs).
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Viable offspring derived from fetal and adult mammalian cells
TL;DR: The birth of lambs from differentiated fetal and adult cells confirms that differentiation of that cell did not involve the irreversible modification of genetic material required for development to term and reinforces previous speculation that by inducing donor cells to become quiescent it will be possible to obtain normal development from a wide variety of differentiated cells.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Muscle Regeneration by Bone Marrow-Derived Myogenic Progenitors
Giuliana Ferrari,Gabriella Cusella,Gabriella Cusella,D. De Angelis,D. De Angelis,M. Coletta,M. Coletta,Egle Paolucci,Egle Paolucci,Anna Stornaiuolo,Anna Stornaiuolo,Giulio Cossu,Giulio Cossu,Fulvio Mavilio,Fulvio Mavilio +14 more
TL;DR: Transplantation of genetically marked bone marrow into immunodeficient mice revealed that marrow-derived cells migrate into areas of induced muscle degeneration, undergo myogenic differentiation, and participate in the regeneration of the damaged fibers.
Journal ArticleDOI
Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell
Diane S. Krause,Neil D. Theise,Michael I. Collector,Octavian Henegariu,Sonya Hwang,Rebekah Gardner,Sara Neutzel,Saul J. Sharkis +7 more
TL;DR: It is shown that rare cells that home to bone marrow can LTR primary and secondary recipients, and this finding may contribute to clinical treatment of genetic disease or tissue repair.