Journal ArticleDOI
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TLDR
It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.Abstract:
Recent studies have demonstrated that transplanted bone marrow cells can turn into unexpected lineages including myocytes, hepatocytes, neurons and many others. A potential problem, however, is that reports discussing such 'transdifferentiation' in vivo tend to conclude donor origin of transdifferentiated cells on the basis of the existence of donor-specific genes such as Y-chromosome markers. Here we demonstrate that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3. Moreover, spontaneously fused bone marrow cells can subsequently adopt the phenotype of the recipient cells, which, without detailed genetic analysis, might be interpreted as 'dedifferentiation' or transdifferentiation.read more
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Stem cells for regenerative medicine: advances in the engineering of tissues and organs
TL;DR: The state of the art in tissue engineering with mesenchymal stem and mesenchYCal progenitor cells with emphasis on bone and cartilage reconstruction is reviewed and several issues of importance, especially with regard to the clinical application of mesenchyal stem cells, are discussed.
Journal ArticleDOI
Pluripotency and nuclear reprogramming
TL;DR: The molecular mechanisms underlying pluripotent stem cells and the currently known methods of inducing pluripotency in somatic cells are reviewed.
Journal ArticleDOI
Transdifferentiation of mesenchymal stem cells into Schwann cell-like myelinating cells
TL;DR: These findings demonstrate that it may be possible to differentiate MSC into therapeutically useful cells for clinical applications and myelinating capacity, and in some cases, single tMSC were able to myelinate more than one axon.
Journal ArticleDOI
Human Mesenchymal Stem Cells Reprogram Adult Cardiomyocytes Toward a Progenitor-Like State Through Partial Cell Fusion and Mitochondria Transfer
Adrien Acquistapace,Adrien Acquistapace,Thierry Bru,Thierry Bru,Pierre-François Lesault,Pierre-François Lesault,Florence Figeac,Florence Figeac,Amélie E. Coudert,Amélie E. Coudert,Olivier Le Coz,Olivier Le Coz,Christo Christov,Christo Christov,Xavier Baudin,Fréderic Auber,René Yiou,René Yiou,Jean-Luc Dubois-Randé,Jean-Luc Dubois-Randé,Anne-Marie Rodriguez,Anne-Marie Rodriguez +21 more
TL;DR: Cardiac hybrid cells were preferentially generated by partial rather than permanent cell fusion and that intercellular structures composed of f‐actin and microtubule filaments were involved in the process, which showed that stem cell mitochondria were transferred into cardiomyocytes, persisted in hybrids and were required for somatic cell reprogramming.
Journal ArticleDOI
Stem cells for ischemic brain injury: A critical review
TL;DR: Evidence to date suggests that continued development of stem cell therapies may ultimately lead to viable treatment options for ischemic brain injury, and careful investigation of the mechanisms underlying trophic benefits will be essential.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Viable offspring derived from fetal and adult mammalian cells
TL;DR: The birth of lambs from differentiated fetal and adult cells confirms that differentiation of that cell did not involve the irreversible modification of genetic material required for development to term and reinforces previous speculation that by inducing donor cells to become quiescent it will be possible to obtain normal development from a wide variety of differentiated cells.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Muscle Regeneration by Bone Marrow-Derived Myogenic Progenitors
Giuliana Ferrari,Gabriella Cusella,Gabriella Cusella,D. De Angelis,D. De Angelis,M. Coletta,M. Coletta,Egle Paolucci,Egle Paolucci,Anna Stornaiuolo,Anna Stornaiuolo,Giulio Cossu,Giulio Cossu,Fulvio Mavilio,Fulvio Mavilio +14 more
TL;DR: Transplantation of genetically marked bone marrow into immunodeficient mice revealed that marrow-derived cells migrate into areas of induced muscle degeneration, undergo myogenic differentiation, and participate in the regeneration of the damaged fibers.
Journal ArticleDOI
Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell
Diane S. Krause,Neil D. Theise,Michael I. Collector,Octavian Henegariu,Sonya Hwang,Rebekah Gardner,Sara Neutzel,Saul J. Sharkis +7 more
TL;DR: It is shown that rare cells that home to bone marrow can LTR primary and secondary recipients, and this finding may contribute to clinical treatment of genetic disease or tissue repair.