Journal ArticleDOI
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TLDR
It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.Abstract:
Recent studies have demonstrated that transplanted bone marrow cells can turn into unexpected lineages including myocytes, hepatocytes, neurons and many others. A potential problem, however, is that reports discussing such 'transdifferentiation' in vivo tend to conclude donor origin of transdifferentiated cells on the basis of the existence of donor-specific genes such as Y-chromosome markers. Here we demonstrate that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3. Moreover, spontaneously fused bone marrow cells can subsequently adopt the phenotype of the recipient cells, which, without detailed genetic analysis, might be interpreted as 'dedifferentiation' or transdifferentiation.read more
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Cell death by mitotic catastrophe : a molecular definition
Maria Castedo,Jean-Luc Perfettini,Thomas Roumier,Karine Andreau,René H. Medema,Guido Kroemer +5 more
TL;DR: It is proposed that mitotic catastrophe results from a combination of deficient cell-cycle checkpoints and cellular damage and failure to arrest the cell cycle before or at mitosis triggers an attempt of aberrant chromosome segregation, which culminates in the activation of the apoptotic default pathway and cellular demise.
Journal ArticleDOI
Biology of hematopoietic stem cells and progenitors: implications for clinical application.
Motonari Kondo,Amy J. Wagers,Markus G. Manz,Susan S. Prohaska,David C. Scherer,Georg F. Beilhack,Judith A. Shizuru,Irving L. Weissman +7 more
TL;DR: Recent rapid advances in understanding the biological nature of hematopoietic stem and progenitor cells have broadened the potential application of these cells in the treatment of diseases.
Journal ArticleDOI
Bone marrow-derived hematopoietic cells generate cardiomyocytes at a low frequency through cell fusion, but not transdifferentiation.
Jens M. Nygren,Stefan Jovinge,Martin Breitbach,Petter Säwén,Wilhelm Röll,Jürgen Hescheler,Jalal Taneera,Bernd K. Fleischmann,Sten Eirik W. Jacobsen +8 more
TL;DR: It is shown that unfractionated bone marrow cells and a purified population of hematopoietic stem and progenitor cells efficiently engraft within the infarcted myocardium, and that bone marrow–derived cardiomyocytes were observed outside the infarsia at a low frequency and were derived exclusively through cell fusion.
Journal ArticleDOI
Retinal Repair by Transplantation of Photoreceptor Precursors
Robert E MacLaren,Rachael A. Pearson,A MacNeil,Ronald H. Douglas,Thomas E. Salt,Masayuki Akimoto,Anand Swaroop,Jane C. Sowden,Robin R. Ali +8 more
TL;DR: It is shown that donor cells can integrate into the adult or degenerating retina if they are taken from the developing retina at a time coincident with the peak of rod genesis, and the ontogenetic stage of donor cells for successful rod photoreceptor transplantation is defined.
Journal ArticleDOI
Autologous mesenchymal stem cell transplantation in stroke patients.
TL;DR: In patients with severe cerebral infarcts, the intravenous infusion of autologous MSCs appears to be a feasible and safe therapy that may improve functional recovery and avoid adverse cell‐related, serological, or imaging‐defined effects.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Viable offspring derived from fetal and adult mammalian cells
TL;DR: The birth of lambs from differentiated fetal and adult cells confirms that differentiation of that cell did not involve the irreversible modification of genetic material required for development to term and reinforces previous speculation that by inducing donor cells to become quiescent it will be possible to obtain normal development from a wide variety of differentiated cells.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
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Muscle Regeneration by Bone Marrow-Derived Myogenic Progenitors
Giuliana Ferrari,Gabriella Cusella,Gabriella Cusella,D. De Angelis,D. De Angelis,M. Coletta,M. Coletta,Egle Paolucci,Egle Paolucci,Anna Stornaiuolo,Anna Stornaiuolo,Giulio Cossu,Giulio Cossu,Fulvio Mavilio,Fulvio Mavilio +14 more
TL;DR: Transplantation of genetically marked bone marrow into immunodeficient mice revealed that marrow-derived cells migrate into areas of induced muscle degeneration, undergo myogenic differentiation, and participate in the regeneration of the damaged fibers.
Journal ArticleDOI
Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell
Diane S. Krause,Neil D. Theise,Michael I. Collector,Octavian Henegariu,Sonya Hwang,Rebekah Gardner,Sara Neutzel,Saul J. Sharkis +7 more
TL;DR: It is shown that rare cells that home to bone marrow can LTR primary and secondary recipients, and this finding may contribute to clinical treatment of genetic disease or tissue repair.