Journal ArticleDOI
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TLDR
It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.Abstract:
Recent studies have demonstrated that transplanted bone marrow cells can turn into unexpected lineages including myocytes, hepatocytes, neurons and many others. A potential problem, however, is that reports discussing such 'transdifferentiation' in vivo tend to conclude donor origin of transdifferentiated cells on the basis of the existence of donor-specific genes such as Y-chromosome markers. Here we demonstrate that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3. Moreover, spontaneously fused bone marrow cells can subsequently adopt the phenotype of the recipient cells, which, without detailed genetic analysis, might be interpreted as 'dedifferentiation' or transdifferentiation.read more
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Isolation of Renal Progenitor Cells from Adult Human Kidney
Benedetta Bussolati,Stefania Bruno,Cristina Grange,Stefano Buttiglieri,Maria Chiara Deregibus,Dario Cantino,Giovanni Camussi +6 more
TL;DR: It is proposed that CD133+ cells from kidney represent a multipotent adult resident stem cell population that may contribute to the repair of renal injury.
Journal ArticleDOI
Regulatory networks in embryo-derived pluripotent stem cells.
Michele Boiani,Hans R. Schöler +1 more
TL;DR: In this paper, the regulatory networks that are responsible for pluripotency in embryo-derived stem cells are investigated and the authors reveal how to achieve phenotypic changes without the genetic manipulation of OCT4, Nanog and other toti/pluripotent associated genes.
Journal ArticleDOI
Human mesenchymal stem cells xenografted directly to rat liver are differentiated into human hepatocytes without fusion.
Yasushi Sato,Hironobu Araki,Hironobu Araki,Junji Kato,Junji Kato,Kiminori Nakamura,Kiminori Nakamura,Yutaka Kawano,Yutaka Kawano,Masayoshi Kobune,Masayoshi Kobune,Tsutomu Sato,Tsutomu Sato,Koji Miyanishi,Koji Miyanishi,Tetsuji Takayama,Tetsuji Takayama,Minoru Takahashi,Minoru Takahashi,Rishu Takimoto,Rishu Takimoto,Satoshi Iyama,Satoshi Iyama,Takuya Matsunaga,Takuya Matsunaga,Seiji Ohtani,Seiji Ohtani,Akihiro Matsuura,Akihiro Matsuura,Hirofumi Hamada,Hirofumi Hamada,Yoshiro Niitsu,Yoshiro Niitsu +32 more
TL;DR: It is demonstrated that MSCs are the most potent component in hepatic differentiation, as revealed by directly xenografting into rat livers.
ORIGINAL ARTICLE A population of very small embryonic-like (VSEL) CXCR4 þ SSEA-1 þ Oct-4 þ stem cells identified in adult bone marrow
TL;DR: In this paper, a homogeneous population of rare (B0.02% of BMMNC) Sca-1 þ linCD45 cells that express by RQ-PCR and immunohistochemistry markers of pluripotent stem cells (PSC) such as SSEA-1, Oct-4, Nanog and Rex-1 were identified.
Journal ArticleDOI
From basics to clinical: a comprehensive review on spinal cord injury.
TL;DR: An extensive overview of SCI research, as well as its clinical component, is provided, covering areas from physiology and anatomy of the spinal cord, neuropathology of the SCI, current clinical options, neuronal plasticity after SCI and a variety of promising neuroprotective, cell-based and combinatorial therapeutic approaches that have recently moved, or are close to clinical testing.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Viable offspring derived from fetal and adult mammalian cells
TL;DR: The birth of lambs from differentiated fetal and adult cells confirms that differentiation of that cell did not involve the irreversible modification of genetic material required for development to term and reinforces previous speculation that by inducing donor cells to become quiescent it will be possible to obtain normal development from a wide variety of differentiated cells.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Muscle Regeneration by Bone Marrow-Derived Myogenic Progenitors
Giuliana Ferrari,Gabriella Cusella,Gabriella Cusella,D. De Angelis,D. De Angelis,M. Coletta,M. Coletta,Egle Paolucci,Egle Paolucci,Anna Stornaiuolo,Anna Stornaiuolo,Giulio Cossu,Giulio Cossu,Fulvio Mavilio,Fulvio Mavilio +14 more
TL;DR: Transplantation of genetically marked bone marrow into immunodeficient mice revealed that marrow-derived cells migrate into areas of induced muscle degeneration, undergo myogenic differentiation, and participate in the regeneration of the damaged fibers.
Journal ArticleDOI
Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell
Diane S. Krause,Neil D. Theise,Michael I. Collector,Octavian Henegariu,Sonya Hwang,Rebekah Gardner,Sara Neutzel,Saul J. Sharkis +7 more
TL;DR: It is shown that rare cells that home to bone marrow can LTR primary and secondary recipients, and this finding may contribute to clinical treatment of genetic disease or tissue repair.