Journal ArticleDOI
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TLDR
It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.Abstract:
Recent studies have demonstrated that transplanted bone marrow cells can turn into unexpected lineages including myocytes, hepatocytes, neurons and many others. A potential problem, however, is that reports discussing such 'transdifferentiation' in vivo tend to conclude donor origin of transdifferentiated cells on the basis of the existence of donor-specific genes such as Y-chromosome markers. Here we demonstrate that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3. Moreover, spontaneously fused bone marrow cells can subsequently adopt the phenotype of the recipient cells, which, without detailed genetic analysis, might be interpreted as 'dedifferentiation' or transdifferentiation.read more
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Pluripotency of mesenchymal stem cells derived from adult marrow
Yuehua Jiang,Balkrishna N. Jahagirdar,R. Lee Reinhardt,Robert E. Schwartz,C. Dirk Keene,Xilma R. Ortiz-Gonzalez,Morayma Reyes,Todd Lenvik,Troy C. Lund,Mark Blackstad,Jingbo Du,Sara Aldrich,Aaron Lisberg,Walter C. Low,David A. Largaespada,Catherine M. Verfaillie +15 more
TL;DR: It is reported here that cells co-purifying with mesenchymal stem cells—termed here multipotent adult progenitor cells or MAPCs—differentiate, at the single cell level, not only into meschymal cells, but also cells with visceral mesoderm, neuroectoderm and endoderm characteristics in vitro.
Journal ArticleDOI
SHED: Stem cells from human exfoliated deciduous teeth
Masako Miura,Stan Gronthos,Mingrui Zhao,Bai Lu,Larry W. Fisher,Pamela Gehron Robey,Songtao Shi +6 more
TL;DR: It is shown that a naturally exfoliated human organ contains a population of stem cells that are completely different from previously identified stem cells, which may be an unexpected unique resource for stem-cell therapies including autologous stem- cell transplantation and tissue engineering.
Journal ArticleDOI
"Stemness": Transcriptional Profiling of Embryonic and Adult Stem Cells
TL;DR: The transcriptional profiles of mouse embryonic, neural, and hematopoietic stem cells were compared to define a genetic program for stem cells and provide a foundation for a more detailed understanding of stem cell biology.
Journal ArticleDOI
Directed Differentiation of Embryonic Stem Cells into Motor Neurons
TL;DR: It is shown that developmentally relevant signaling factors can induce mouse embryonic stem cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo.
Journal ArticleDOI
Haematopoietic stem cells adopt mature haematopoietic fates in ischaemic myocardium
Leora B. Balsam,Amy J. Wagers,Julie L. Christensen,Theo Kofidis,Irving L. Weissman,Robert C. Robbins +5 more
TL;DR: The data suggest that even in the microenvironment of the injured heart, c-kit-enriched BM cells, Lin- c- Kit+ BM cells and c-Kit+ Thy1.1lo Lin- Sca-1+ long-term reconstituting haematopoietic stem cells adopt only traditional haem atopoetic fates.
References
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Journal ArticleDOI
Dnmt3L and the establishment of maternal genomic imprints.
TL;DR: Bisulfite genomic sequencing of DNA from oocytes and embryos showed that removal of Dnmt3L prevented methylation of sequences that are normally maternally methylated, and the defect was specific to imprinted regions, and global genome methylation levels were not affected.
Journal ArticleDOI
Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity.
Hiroshi Kawasaki,Kenji Mizuseki,Satomi Nishikawa,Satoshi Kaneko,Yoshihisa Kuwana,Shigetada Nakanishi,Shin-Ichi Nishikawa,Yoshiki Sasai +7 more
TL;DR: Neural induction by SDIA provides a new powerful tool for both basic neuroscience research and therapeutic applications.
Journal ArticleDOI
Liver from bone marrow in humans.
Neil D. Theise,Manjunath Nimmakayalu,Rebekah Gardner,Peter B. Illei,Glyn Morgan,Lewis W. Teperman,Octavian Henegariu,Diane S. Krause +7 more
TL;DR: It is shown that in humans, hepatocytes and cholangiocytes can be derived from extrahepatic circulating stem cells, probably of bone marrow origin, and such “transdifferentiation” can replenish large numbers of hepatic parenchymal cells.
Neurotechnique Induction of Midbrain Dopaminergic Neurons from ES Cells by Stromal Cell-Derived Inducing Activity
Hiroshi Kawasaki,Kenji Mizuseki,Satomi Nishikawa,Satoshi Kaneko,Yoshihisa Kuwana,Shigetada Nakanishi,Shin-Ichi Nishikawa,Yoshiki Sasai,Kyowa Hakko Kogyo +8 more
TL;DR: In this article, an efficient system for in vitro differentiation of mouse ES cells without use of either retinoic acid or embryoid vitro neural differentiation in a serum- bodies was introduced. But this method requires neither EBs nor RA treat- phogen in Xenopus.
Journal ArticleDOI
Erratum: Viable offspring derived from fetal and adult mammalian cells
TL;DR: In this Letter in the 27 February issue, a production error led to the image for part b of Fig. 1 (fetal fibroblasts) being used twice, as parts b and c.