Journal ArticleDOI
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TLDR
It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.Abstract:
Recent studies have demonstrated that transplanted bone marrow cells can turn into unexpected lineages including myocytes, hepatocytes, neurons and many others. A potential problem, however, is that reports discussing such 'transdifferentiation' in vivo tend to conclude donor origin of transdifferentiated cells on the basis of the existence of donor-specific genes such as Y-chromosome markers. Here we demonstrate that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3. Moreover, spontaneously fused bone marrow cells can subsequently adopt the phenotype of the recipient cells, which, without detailed genetic analysis, might be interpreted as 'dedifferentiation' or transdifferentiation.read more
Citations
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Journal ArticleDOI
Cell-fusion-mediated somatic-cell reprogramming: a mechanism for tissue regeneration.
Frederic Lluis,Maria Pia Cosma +1 more
TL;DR: This work has shown that injury to a tissue can enhance spontaneous cell–cell fusion events, and if this process is deregulated, this would provide a mechanism for cancer development.
Journal ArticleDOI
Nanopatterned bulk metallic glass-based biomaterials modulate macrophage polarization.
Mahdis Shayan,Jagannath Padmanabhan,Aaron H. Morris,Bettina Cheung,Ryan G Smith,Jan Schroers,Themis R. Kyriakides +6 more
TL;DR: This study demonstrates that nanopatterning of BMG implants is a promising technique to selectively polarize macrophages to modulate the immune response, and also presents an effective tool to study mechanisms of macrophage polarization and function.
Journal ArticleDOI
The role of circulating precursors in vascular repair and lesion formation.
Masataka Sata,Masataka Sata,Daiju Fukuda,Kimie Tanaka,Yukari Kaneda,Yukari Kaneda,Hisako Yashiro,Ibuki Shirakawa,Ibuki Shirakawa +8 more
TL;DR: Recent findings on circulating vascular progenitor cells are overviews and potential therapeutic strategies that target these cells to treat occlusive vascular diseases are described.
Journal ArticleDOI
Fusion of Human Hematopoietic Progenitor Cells and Murine Cardiomyocytes Is Mediated by α4β1 Integrin/Vascular Cell Adhesion Molecule-1 Interaction
TL;DR: It is shown that hypoxia and cytokines increase fusion of human peripheral blood CD34-positive cells and murine cardiomyocytes in vitro by up to 7-fold, and this is blocked by anti-&agr;4&bgr;1 or anti-vascular cell adhesion molecule (VCAM)-1, leading to cell cycle reentry and cellular proliferation.
Book ChapterDOI
Retinoids in lung development and regeneration.
TL;DR: Recent data from animal studies suggest that retinoids can induce regeneration of alveoli in young adults, which, if relevant to humans, might be the first potential treatment for diseases such as emphysema or bronchopulmonary dysplasia.
References
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Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
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Journal ArticleDOI
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TL;DR: The birth of lambs from differentiated fetal and adult cells confirms that differentiation of that cell did not involve the irreversible modification of genetic material required for development to term and reinforces previous speculation that by inducing donor cells to become quiescent it will be possible to obtain normal development from a wide variety of differentiated cells.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Muscle Regeneration by Bone Marrow-Derived Myogenic Progenitors
Giuliana Ferrari,Gabriella Cusella,Gabriella Cusella,D. De Angelis,D. De Angelis,M. Coletta,M. Coletta,Egle Paolucci,Egle Paolucci,Anna Stornaiuolo,Anna Stornaiuolo,Giulio Cossu,Giulio Cossu,Fulvio Mavilio,Fulvio Mavilio +14 more
TL;DR: Transplantation of genetically marked bone marrow into immunodeficient mice revealed that marrow-derived cells migrate into areas of induced muscle degeneration, undergo myogenic differentiation, and participate in the regeneration of the damaged fibers.
Journal ArticleDOI
Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell
Diane S. Krause,Neil D. Theise,Michael I. Collector,Octavian Henegariu,Sonya Hwang,Rebekah Gardner,Sara Neutzel,Saul J. Sharkis +7 more
TL;DR: It is shown that rare cells that home to bone marrow can LTR primary and secondary recipients, and this finding may contribute to clinical treatment of genetic disease or tissue repair.