Journal ArticleDOI
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TLDR
It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.Abstract:
Recent studies have demonstrated that transplanted bone marrow cells can turn into unexpected lineages including myocytes, hepatocytes, neurons and many others. A potential problem, however, is that reports discussing such 'transdifferentiation' in vivo tend to conclude donor origin of transdifferentiated cells on the basis of the existence of donor-specific genes such as Y-chromosome markers. Here we demonstrate that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3. Moreover, spontaneously fused bone marrow cells can subsequently adopt the phenotype of the recipient cells, which, without detailed genetic analysis, might be interpreted as 'dedifferentiation' or transdifferentiation.read more
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Pluripotency of mesenchymal stem cells derived from adult marrow
Yuehua Jiang,Balkrishna N. Jahagirdar,R. Lee Reinhardt,Robert E. Schwartz,C. Dirk Keene,Xilma R. Ortiz-Gonzalez,Morayma Reyes,Todd Lenvik,Troy C. Lund,Mark Blackstad,Jingbo Du,Sara Aldrich,Aaron Lisberg,Walter C. Low,David A. Largaespada,Catherine M. Verfaillie +15 more
TL;DR: It is reported here that cells co-purifying with mesenchymal stem cells—termed here multipotent adult progenitor cells or MAPCs—differentiate, at the single cell level, not only into meschymal cells, but also cells with visceral mesoderm, neuroectoderm and endoderm characteristics in vitro.
Journal ArticleDOI
SHED: Stem cells from human exfoliated deciduous teeth
Masako Miura,Stan Gronthos,Mingrui Zhao,Bai Lu,Larry W. Fisher,Pamela Gehron Robey,Songtao Shi +6 more
TL;DR: It is shown that a naturally exfoliated human organ contains a population of stem cells that are completely different from previously identified stem cells, which may be an unexpected unique resource for stem-cell therapies including autologous stem- cell transplantation and tissue engineering.
Journal ArticleDOI
"Stemness": Transcriptional Profiling of Embryonic and Adult Stem Cells
TL;DR: The transcriptional profiles of mouse embryonic, neural, and hematopoietic stem cells were compared to define a genetic program for stem cells and provide a foundation for a more detailed understanding of stem cell biology.
Journal ArticleDOI
Directed Differentiation of Embryonic Stem Cells into Motor Neurons
TL;DR: It is shown that developmentally relevant signaling factors can induce mouse embryonic stem cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo.
Journal ArticleDOI
Haematopoietic stem cells adopt mature haematopoietic fates in ischaemic myocardium
Leora B. Balsam,Amy J. Wagers,Julie L. Christensen,Theo Kofidis,Irving L. Weissman,Robert C. Robbins +5 more
TL;DR: The data suggest that even in the microenvironment of the injured heart, c-kit-enriched BM cells, Lin- c- Kit+ BM cells and c-Kit+ Thy1.1lo Lin- Sca-1+ long-term reconstituting haematopoietic stem cells adopt only traditional haem atopoetic fates.
References
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Journal ArticleDOI
Bidirectional imprinting of a single gene: GNAS1 encodes maternally, paternally, and biallelically derived proteins
TL;DR: This work investigates the allelic origin of other mRNAs derived from GNAS1, a gene showing simultaneous imprinting in both the paternal and maternal directions, and finds this gene to be remarkable in the complexity of its allele-specific regulation.
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Peg1/Mest imprinted gene on chromosome 6 identified by cDNA subtraction hybridization.
T Kaneko-Ishino,Yoshimi Kuroiwa,Naoki Miyoshi,Takashi Kohda,R Suzuki,Minesuke Yokoyama,S. Viville,Sheila C. Barton,Fumitoshi Ishino,M. A. Surani +9 more
TL;DR: Peg1 (paternally expressed gene 1) or Mest, the first imprinted gene found on the mouse chromosome 6, may contribute to the lethality of parthenogenones and of embryos with a maternal duplication for the proximal chromosome 6.
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The human GNAS1 gene is imprinted and encodes distinct paternally and biallelically expressed G proteins.
Bruce E. Hayward,Mamoru Kamiya,Lisa Strain,Veronica Moran,Roderick Campbell,Yoshihide Hayashizaki,David T. Bonthron +6 more
TL;DR: It is shown that, although Gsalpha expression (directed by the promoter upstream of exon 1) is biallelic, GNAS1 is indeed imprinted in a promoter-specific fashion, and may contribute to the anomalous inheritance of PHP Ia.
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The purinergic P2Z receptor of human macrophage cells. Characterization and possible physiological role.
TL;DR: The data suggest that human macrophages express rIFN-gamma-modulated purinergic P2Z receptors in vitro and hint at a role for these plasma membrane molecules in the generation of macrophage polykarions.
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Evolution of imprinting mechanisms: the battle of the sexes begins in the zygote.
TL;DR: It is shown that, for most genes from either category, the methylation imprint is derived from the oocyte, and an explanation is provided for this intriguing asymmetry.