Journal ArticleDOI
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TLDR
It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.Abstract:
Recent studies have demonstrated that transplanted bone marrow cells can turn into unexpected lineages including myocytes, hepatocytes, neurons and many others. A potential problem, however, is that reports discussing such 'transdifferentiation' in vivo tend to conclude donor origin of transdifferentiated cells on the basis of the existence of donor-specific genes such as Y-chromosome markers. Here we demonstrate that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3. Moreover, spontaneously fused bone marrow cells can subsequently adopt the phenotype of the recipient cells, which, without detailed genetic analysis, might be interpreted as 'dedifferentiation' or transdifferentiation.read more
Citations
More filters
Journal ArticleDOI
Granulocyte-colony stimulating factor for mobilizing bone marrow stem cells in subacute stroke: the stem cell trial of recovery enhancement after stroke 2 randomized controlled trial.
Timothy J. England,Maryam Abaei,Dorothee P. Auer,James Lowe,D. Rhodri E. Jones,Gillian M. Sare,Marion F Walker,Philip M.W. Bath +7 more
TL;DR: This randomized, double-blind, placebo-controlled trial suggests that G-CSF is safe when administered subacutely and it is feasible to label and readminister iron-labeled CD34+ cells in patients with ischemic stroke.
Journal ArticleDOI
Rat adult stem cells (marrow stromal cells) engraft and differentiate in chick embryos without evidence of cell fusion
TL;DR: Adult stem cells, referred to as mesenchymal stem cells or marrow stromal cells, from rat marrow, were infused into chick embryos and engrafted into many tissues, and no multinuclear cells were detected.
Journal ArticleDOI
Electro-acupuncture promotes survival, differentiation of the bone marrow mesenchymal stem cells as well as functional recovery in the spinal cord-transected rats
Ying Ding,Qing Yan,Jing Wen Ruan,Yanqing Zhang,Wen Jie Li,Yu Jiao Zhang,Yan Li,Hongxin Dong,Yuan Shan Zeng +8 more
TL;DR: MSCs transplantation combined with EA treatment could promote axonal regeneration and partial locomotor functional recovery in the transected spinal cord in rats and indicate a promising avenue of treatment of spinal cord injury.
Journal ArticleDOI
Regeneration of dystrophin -expressing myocytes in the mdx heart by skeletal muscle stem cells
Thomas Payne,Hideki Oshima,Hideki Oshima,Tetsuro Sakai,Tetsuro Sakai,Yiqun Ling,Yiqun Ling,Burhan Gharaibeh,Burhan Gharaibeh,James H. Cummins,Johnny Huard +10 more
TL;DR: It is demonstrated that MDSC transplantation warrants further investigation as a potential therapy for cardiac dysfunction in DMD, with sex-mismatched donors and hosts revealing that many donor-derived cells that acquired a cardiac phenotype did so through fusion with host cardiomyocytes.
Journal ArticleDOI
Hepatic progenitor cells, stem cells, and AFP expression in models of liver injury.
Wolf D Kuhlmann,Peter Peschke +1 more
TL;DR: Adult hepatocytes and liver‐cell progenitors play a role in restoring liver tissue after injury and the heterogeneity of phenotypic marker (AFP) correlated with a process of retrodifferentiation.
References
More filters
Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Viable offspring derived from fetal and adult mammalian cells
TL;DR: The birth of lambs from differentiated fetal and adult cells confirms that differentiation of that cell did not involve the irreversible modification of genetic material required for development to term and reinforces previous speculation that by inducing donor cells to become quiescent it will be possible to obtain normal development from a wide variety of differentiated cells.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Muscle Regeneration by Bone Marrow-Derived Myogenic Progenitors
Giuliana Ferrari,Gabriella Cusella,Gabriella Cusella,D. De Angelis,D. De Angelis,M. Coletta,M. Coletta,Egle Paolucci,Egle Paolucci,Anna Stornaiuolo,Anna Stornaiuolo,Giulio Cossu,Giulio Cossu,Fulvio Mavilio,Fulvio Mavilio +14 more
TL;DR: Transplantation of genetically marked bone marrow into immunodeficient mice revealed that marrow-derived cells migrate into areas of induced muscle degeneration, undergo myogenic differentiation, and participate in the regeneration of the damaged fibers.
Journal ArticleDOI
Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell
Diane S. Krause,Neil D. Theise,Michael I. Collector,Octavian Henegariu,Sonya Hwang,Rebekah Gardner,Sara Neutzel,Saul J. Sharkis +7 more
TL;DR: It is shown that rare cells that home to bone marrow can LTR primary and secondary recipients, and this finding may contribute to clinical treatment of genetic disease or tissue repair.