Journal ArticleDOI
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TLDR
It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.Abstract:
Recent studies have demonstrated that transplanted bone marrow cells can turn into unexpected lineages including myocytes, hepatocytes, neurons and many others. A potential problem, however, is that reports discussing such 'transdifferentiation' in vivo tend to conclude donor origin of transdifferentiated cells on the basis of the existence of donor-specific genes such as Y-chromosome markers. Here we demonstrate that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3. Moreover, spontaneously fused bone marrow cells can subsequently adopt the phenotype of the recipient cells, which, without detailed genetic analysis, might be interpreted as 'dedifferentiation' or transdifferentiation.read more
Citations
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Increase of Bone Marrow-Derived Secretory Lineage Epithelial Cells During Regeneration in the Human Intestine
Tomoko Matsumoto,Ryuichi Okamoto,Tomoharu Yajima,Takehiko Mori,Shinichiro Okamoto,Yasuo Ikeda,Makio Mukai,Motomi Yamazaki,Shigeru Oshima,Kiichiro Tsuchiya,Tetsuya Nakamura,Takanori Kanai,Hideyuki Okano,Johji Inazawa,Toshifumi Hibi,Mamoru Watanabe +15 more
TL;DR: BM-derived epithelial cells arise via a mechanism other than cell fusion and rarely give rise to stem cells, however, a small proportion of these cells express proliferation markers, and a majority reside as terminally differentiated cells.
Journal ArticleDOI
In vivo genetic selection of renal proximal tubules.
Patrice K. Held,Muhsen Al-Dhalimy,Holger Willenbring,Yassmine Akkari,Shuguang Jiang,Yumi Torimaru,Susan B. Olson,William H. Fleming,Milton J. Finegold,Markus Grompe +9 more
TL;DR: An animal model in which cells genetically resistant to a toxic intermediate of tyrosine metabolism, homogentisic acid (HGA), were able to repopulate the damaged proximal tubule epithelium of mice with fumarylacetoacetate hydrolase (Fah) deficiency is developed.
Journal ArticleDOI
Functional neural stem cells derived from adult bone marrow.
TL;DR: The ability of adult bone marrow progenitors to generate self-renewing, functional neural stem cells is demonstrated, validating this approach as an alternative source of long-lasting neural stem Cells with therapeutic implications in neurodegenerative diseases.
Journal ArticleDOI
Adult‐derived stem cells and their potential for use in tissue repair and molecular medicine
Henry E. Young,Cécile Duplàa,Ryan Katz,Tina L Thompson,Kristina C. Hawkins,Angel N. Boev,Nicholas L. Henson,Matthew Heaton,Rajiv Sood,Dennis W. Ashley,Christopher L. Stout,Joe H. Morgan,Peter N. Uchakin,Marylen Rimando,Gypsy F. Long,Crystal Thomas,Jee-In Yoon,Jee-In Yoon,Ji Eun Park,Ji Eun Park,Darren J. Hunt,Nancy M. Walsh,Josh C. Davis,Joel E. Lightner,Anna M. Hutchings,Meredith L. Murphy,Elizabeth L. Boswell,Jessica A. McAbee,Brandon M. Gray,Janet F. Piskurich,Lisa Blake,Julie A. Collins,Catherine Moreau,Douglas C. Hixson,Frank P. Bowyer,Asa C. Black +35 more
TL;DR: The potential roles of adult‐derived precursor cells as building blocks for tissue repair and as delivery vehicles for molecular medicine are discussed by discussing the three categories of precursor cells present within adults.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Viable offspring derived from fetal and adult mammalian cells
TL;DR: The birth of lambs from differentiated fetal and adult cells confirms that differentiation of that cell did not involve the irreversible modification of genetic material required for development to term and reinforces previous speculation that by inducing donor cells to become quiescent it will be possible to obtain normal development from a wide variety of differentiated cells.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Muscle Regeneration by Bone Marrow-Derived Myogenic Progenitors
Giuliana Ferrari,Gabriella Cusella,Gabriella Cusella,D. De Angelis,D. De Angelis,M. Coletta,M. Coletta,Egle Paolucci,Egle Paolucci,Anna Stornaiuolo,Anna Stornaiuolo,Giulio Cossu,Giulio Cossu,Fulvio Mavilio,Fulvio Mavilio +14 more
TL;DR: Transplantation of genetically marked bone marrow into immunodeficient mice revealed that marrow-derived cells migrate into areas of induced muscle degeneration, undergo myogenic differentiation, and participate in the regeneration of the damaged fibers.
Journal ArticleDOI
Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell
Diane S. Krause,Neil D. Theise,Michael I. Collector,Octavian Henegariu,Sonya Hwang,Rebekah Gardner,Sara Neutzel,Saul J. Sharkis +7 more
TL;DR: It is shown that rare cells that home to bone marrow can LTR primary and secondary recipients, and this finding may contribute to clinical treatment of genetic disease or tissue repair.