Journal ArticleDOI
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Naohiro Terada,Takashi Hamazaki,Masahiro Oka,Masanori Hoki,Diana M. Mastalerz,Yuka Nakano,Edwin M. Meyer,Laurence Morel,Bryon E. Petersen,Edward W. Scott +9 more
TLDR
It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.Abstract:
Recent studies have demonstrated that transplanted bone marrow cells can turn into unexpected lineages including myocytes, hepatocytes, neurons and many others. A potential problem, however, is that reports discussing such 'transdifferentiation' in vivo tend to conclude donor origin of transdifferentiated cells on the basis of the existence of donor-specific genes such as Y-chromosome markers. Here we demonstrate that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3. Moreover, spontaneously fused bone marrow cells can subsequently adopt the phenotype of the recipient cells, which, without detailed genetic analysis, might be interpreted as 'dedifferentiation' or transdifferentiation.read more
Citations
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Stem cells, embryos, and the environment: a context for both science and ethics
Cindy Towns,Jones Dg +1 more
TL;DR: It is argued that blastocysts (early embryos) and embryonic stem cells are only totipotent if they can develop within an appropriate environment, and in the absence of this, they are merely pluripotent.
Journal ArticleDOI
Hepatocyte progenitors in man and in rodents – multiple pathways, multiple candidates
TL;DR: A review of potential liver‐cell progenitors from bone marrow, peripheral blood, cord blood, foetal liver, adult liver and embryonic stem cells finds differences and similarities found among cells isolated from rodents and humans.
Journal ArticleDOI
Evidence for transdifferentiation of human bone marrow-derived stem cells: recent progress and controversies.
Helen Tao,David D.F. Ma +1 more
TL;DR: This review summarises recent progresses and controversies in transdifferentiation of adult bone marrow‐derived stem cells to non‐haemopoietic tissues.
Journal ArticleDOI
Bone marrow mononuclear cells reduce seizure frequency and improve cognitive outcome in chronic epileptic rats.
Gianina Teribele Venturin,Samuel Greggio,Daniel Rodrigo Marinowic,Gabriele Zanirati,Martín Cammarota,Denise Cantarelli Machado,Jaderson Costa DaCosta +6 more
TL;DR: Data show that bone marrow mononuclear cells reduced the frequency of seizures and improved the learning and long-term spatial memory impairments of epileptic rats, indicating that BMMCs could represent a promising therapeutic option in the management of chronic temporal lobe epilepsy.
Journal ArticleDOI
Transplantation-mediated strategies to promote axonal regeneration following spinal cord injury.
Xiao Ming Xu,Stephen M. Onifer +1 more
TL;DR: Information is reviewed about the ability and potential, particularly for traumatic spinal cord injury, that neural and non-neural tissue and cell transplantation has to replace lost neurons and glia, to reconstruct damaged neural circuitry, and to restore neurotransmitters, hormones, neurotrophic factors, and neurotransmission.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Viable offspring derived from fetal and adult mammalian cells
TL;DR: The birth of lambs from differentiated fetal and adult cells confirms that differentiation of that cell did not involve the irreversible modification of genetic material required for development to term and reinforces previous speculation that by inducing donor cells to become quiescent it will be possible to obtain normal development from a wide variety of differentiated cells.
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Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
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Muscle Regeneration by Bone Marrow-Derived Myogenic Progenitors
Giuliana Ferrari,Gabriella Cusella,Gabriella Cusella,D. De Angelis,D. De Angelis,M. Coletta,M. Coletta,Egle Paolucci,Egle Paolucci,Anna Stornaiuolo,Anna Stornaiuolo,Giulio Cossu,Giulio Cossu,Fulvio Mavilio,Fulvio Mavilio +14 more
TL;DR: Transplantation of genetically marked bone marrow into immunodeficient mice revealed that marrow-derived cells migrate into areas of induced muscle degeneration, undergo myogenic differentiation, and participate in the regeneration of the damaged fibers.
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Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell
Diane S. Krause,Neil D. Theise,Michael I. Collector,Octavian Henegariu,Sonya Hwang,Rebekah Gardner,Sara Neutzel,Saul J. Sharkis +7 more
TL;DR: It is shown that rare cells that home to bone marrow can LTR primary and secondary recipients, and this finding may contribute to clinical treatment of genetic disease or tissue repair.