Cancer Stem Cells in Squamous Cell Carcinoma Switch between Two Distinct Phenotypes That Are Preferentially Migratory or Proliferative
Adrian Biddle,Xiao Liang,Luke Gammon,Bilal Fazil,Lisa J. Harper,Helena Emich,Daniela Elena Costea,Ian C. Mackenzie +7 more
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TLDR
A need to define therapeutic targets that can eradicate both EMT and self-renewing CSC variants to achieve effective SCC treatment is suggested.Abstract:
Epithelial-to-mesenchymal transition (EMT) is an important driver of tumor invasion and metastasis, which causes many cancer deaths. Cancer stem cells (CSC) that maintain and initiate tumors have also been implicated in invasion and metastasis, but whether EMT is an important contributor to CSC function is unclear. In this study, we investigated whether a population of CSCs that have undergone EMT (EMT CSCs) exists in squamous cell carcinoma (SCC). We also determined whether a separate population of CSCs that retain epithelial characteristics (non-EMT CSCs) is also present. Our studies revealed that self-renewing CSCs in SCC include two biologically-distinct phenotypes. One phenotype, termed CD44(high)ESA(high), was proliferative and retained epithelial characteristics (non-EMT CSCs), whereas the other phenotype, termed CD44(high)ESA(low), was migratory and had mesenchymal traits characteristic of EMT CSCs. We found that non-EMT and EMT CSCs could switch their epithelial or mesenchymal traits to reconstitute the cellular heterogeneity which was characteristic of CSCs. However, the ability of EMT CSCs to switch to non-EMT character was restricted to cells that were also ALDH1(+), implying that only ALDH1(+) EMT cells had the ability to seed a new epithelial tumor. Taken together, our findings highlight the identification of two distinct CSC phenotypes and suggest a need to define therapeutic targets that can eradicate both of these variants to achieve effective SCC treatment.read more
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siMTA1-Loaded Exosomes Enhanced Chemotherapeutic Effect of Gemcitabine in Luminal-b Type Breast Cancer by Inhibition of EMT/HIF-α and Autophagy Pathways.
TL;DR: This research shows that higher expression of MTA1 accompanies with worse prognosis in luminal-b breast cancer, and builds the siMTA1-loaded exosomes, which increases the gemcitabine-mediated tumor growth inhibition effect in vivo.
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HMGA2-Snai2 axis regulates tumorigenicity and stemness of head and neck squamous cell carcinoma.
Zhongwu Li,Xiang Wu,Jin Li,Shijin Yu,Xueping Ke,Ting-kai Yan,Yumin Zhu,Jie Chen,Jianrong Yang +8 more
TL;DR: In this article , the role and underlying molecular targets of high mobility group AT-hook 2 (HMGA2) in the progression and CSCs regulation of head and neck squamous cell carcinoma (HNSCC) were investigated.
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Decreased expression of cell adhesion genes in cancer stem-like cells isolated from primary oral squamous cell carcinomas.
Amrendra Mishra,Amrendra Mishra,Harshini Sriram,Pinal Chandarana,Vivek Tanavde,Vivek Tanavde,Rekha V. Kumar,Ashok Gopinath,Raman Govindarajan,S Ramaswamy,Subhashini Sadasivam +10 more
TL;DR: Lower expression of the cell–cell adhesion molecule PCDH18 correlated with poorer overall survival in the The Cancer Genome Atlas–Head and Neck Squamous Cell Carcinoma data highlighting it as a potential negative prognostic factor in this cancer.
Cancer stem-like cells enriched with CD29 and CD44 markers exhibit molecular characteristics with epithelial-mesenchymal transition in squamous cell carcinoma
TL;DR: In this paper, the combination of CD29 and CD44 could be used to identify cancer stem-like cells undergoing EMT in squa-mous cell carcinoma (SCC) and compare the molecular differences between CD29high/CD44high and CD29low/ CD44low cells in SCC.
Journal ArticleDOI
DEAD-box helicase 27 plays a tumor-promoter role by regulating the stem cell-like activity of human colorectal cancer cells
Chunxing Yang,Daojiang Li,Yang Bai,Shenglei Song,Peicheng Yan,Runliu Wu,Yi Zhang,Gui Hu,Changwei Lin,Xiaorong Li,Lihuang Huang +10 more
TL;DR: DDX27 may play a tumorpromoter role of CRC by regulating the stem cell-like activity of CRC cells by downregulating the gene expression of known CSC markers in CRC cells, inhibit sphere-formation ability, and promote colonosphere differentiation.
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Journal Article
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TL;DR: A mechanistic link between Twist, EMT, and tumor metastasis is established, suggesting that Twist contributes to metastasis by promoting an epithelial-mesenchymal transition (EMT).