Diagnostic performances and thresholds: The key to harmonization in serological SARS-CoV-2 assays?
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TLDR
The results obtained indicate that, for CLIA assays, it might be possible to define thresholds that improve the negative likelihood ratio, and improved the overall inter-assay agreement, paving the way to a better harmonization of serologic tests.About:
This article is published in Clinica Chimica Acta.The article was published on 2020-10-01 and is currently open access. It has received 102 citations till now.read more
Citations
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Journal ArticleDOI
Robust T Cell Immunity in Convalescent Individuals with Asymptomatic or Mild COVID-19.
Takuya Sekine,André Perez-Potti,Olga Rivera-Ballesteros,Kristoffer Strålin,Jean Baptiste Gorin,Annika Olsson,Sian Llewellyn-Lacey,Habiba Kamal,Gordana Bogdanovic,Sandra Muschiol,David J. Wullimann,Tobias Kammann,Johanna Emgård,Tiphaine Parrot,Elin Folkesson,Olav Rooyackers,Lars Eriksson,Jan-Inge Henter,Anders Sönnerborg,Tobias Allander,Jan Albert,Morten Nielsen,Jonas Klingström,Sara Gredmark-Russ,Niklas K. Björkström,Johan K. Sandberg,David Price,Hans-Gustaf Ljunggren,Soo Aleman,Marcus Buggert +29 more
TL;DR: It is shown that SARS-CoV-2 elicits robust, broad and highly functional memory T cell responses, suggesting that natural exposure or infection may prevent recurrent episodes of severe COVID-19.
Journal ArticleDOI
Performance characteristics of five immunoassays for SARS-CoV-2: a head-to-head benchmark comparison
Mark A. Ainsworth,Monique Andersson,Monique Andersson,Kathryn Auckland,J K Baillie,Eleanor Barnes,Sally Beer,A Beveridge,Sadia Bibi,L Blackwell,M Borak,Abbie Bown,Tim Brooks,Nicola A. Burgess-Brown,S Camara,Matthew Catton,K K Chau,Thomas Christott,Elizabeth A. Clutterbuck,Jesse Coker,Richard J. Cornall,Stuart Cox,D Crawford-Jones,Derrick W. Crook,Silvia D'Arcangelo,W Dejnirattsai,Dequaire Jmm.,S Dimitriadis,Kate E. Dingle,George Doherty,Christina Dold,Tao Dong,Susanna Dunachie,Daniel Ebner,M Emmenegger,A Espinosa,David W Eyre,Rory Fairhead,S Fassih,Conor Feehily,S Felle,Alejandra Fernández-Cid,M Fernandez Mendoza,T H Foord,T Fordwoh,D Fox McKee,John Frater,V Gallardo Sanchez,N Gent,D Georgiou,Christopher J. Groves,Bassam Hallis,P M Hammond,Stephanie B Hatch,Heli Harvala,Jennifer Hill,Sarah Hoosdally,B Horsington,Alison Howarth,Tim James,Katie Jeffery,E Y Jones,Anita Justice,F Karpe,James Kavanagh,D S Kim,R Kirton,Paul Klenerman,Julian C. Knight,L Koukouflis,Andrew J Kwok,U Leuschner,R Levin,A Linder,T Lockett,Sheila F Lumley,S Marinou,Brian D. Marsden,José William Martínez,L Martins Ferreira,L Mason,Philippa C Matthews,Alexander J. Mentzer,A Mobbs,Juthathip Mongkolsapaya,J Morrow,Mukhopadhyay Smm.,Matt J. Neville,Sarah Oakley,Marta Oliveira,Ashley Otter,K Paddon,J Pascoe,Y Peng,E Perez,Prem Perumal,Peto Tea.,H Pickford,Rutger J. Ploeg,Andrew J. Pollard,Alex J. Richardson,Thomas G Ritter,Devender Roberts,Gillian Rodger,Christine S. Rollier,Cathy Rowe,Justine K. Rudkin,Gavin R. Screaton,Malcolm G Semple,Alex Sienkiewicz,L Silva-Reyes,Donal T. Skelly,A Sobrino Diaz,L Stafford,Lisa Stockdale,Nicole Stoesser,Teresa L Street,David I. Stuart,Angela Sweed,Aimee R. Taylor,H Thraves,H P Tsang,M K Verheul,Richard Vipond,Timothy M Walker,Susan Wareing,Y Warren,Carrow I. Wells,C Wilson,K Withycombe,R K Young +130 more
TL;DR: Four commercial, widely available assays and a scalable 384-well ELISA can be used for SARS-CoV-2 serological testing to achieve sensitivity and specificity of at least 98%.
Posted ContentDOI
Robust T cell immunity in convalescent individuals with asymptomatic or mild COVID-19
Takuya Sekine,André Perez-Potti,Olga Rivera-Ballesteros,Kristoffer Strålin,Jean-Baptiste Gorin,Annika Olsson,Sian Llewellyn-Lacey,Habiba Kamal,Gordana Bogdanovic,Sandra Muschiol,David J. Wullimann,Tobias Kammann,Johanna Emgård,Tiphaine Parrot,Elin Folkesson,Olav Rooyackers,Olav Rooyackers,Lars Eriksson,Lars Eriksson,Anders Sönnerborg,Tobias Allander,Tobias Allander,Jan Albert,Jan Albert,Morten Nielsen,Jonas Klingström,Sara Gredmark-Russ,Niklas K. Björkström,Johan K. Sandberg,David Price,Hans-Gustaf Ljunggren,Soo Aleman,Marcus Buggert +32 more
TL;DR: The collective dataset shows that SARS-CoV-2 elicits robust memory T cell responses akin to those observed in the context of successful vaccines, suggesting that natural exposure or infection may prevent recurrent episodes of severe COVID-19 also in seronegative individuals.
Journal ArticleDOI
Post-COVID-19 symptoms 6 months after acute infection among hospitalized and non-hospitalized patients.
Maddalena Peghin,Alvisa Palese,Margherita Venturini,Maria De Martino,Valentina Gerussi,Elena Graziano,Giulia Bontempo,Francesco Marrella,Alberto Tommasini,Martina Fabris,Francesco Curcio,Miriam Isola,Carlo Tascini +12 more
TL;DR: The prevalence of post-coronavirus disease 2019 (COVID-19) syndrome was 40.2% (241/599) and the presence of IgG antibodies against SARS-CoV-2 was significantly associated with the occurrence of post COVID19 syndrome (OR 2.56, 95% CI 1.48-4.38, p 0.004) as mentioned in this paper.
Journal ArticleDOI
Analytical and clinical performances of five immunoassays for the detection of SARS-CoV-2 antibodies in comparison with neutralization activity.
Andrea Padoan,Francesco Bonfante,Matteo Pagliari,Alessio Bortolami,Davide Negrini,Silvia Zuin,Dania Bozzato,Chiara Cosma,Laura Sciacovelli,Mario Plebani +9 more
TL;DR: All immunoassays had an excellent specificity, whereas sensitivity varied across immunosorbent assays, depending strongly on the time interval between symptoms onset and sample collection, according to the SARS-CoV-2 virus.
References
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Kelvin K. W. To,Owen Tak Yin Tsang,Wai Shing Leung,Anthony Raymond Tam,Tak Chiu Wu,David Christopher Lung,Cyril C. Y. Yip,Jian Piao Cai,Jacky Man Chun Chan,Thomas Shiu Hong Chik,Daphne Pui-Ling Lau,Chris Yau Chung Choi,Lin Lei Chen,Wan Mui Chan,Kwok-Hung Chan,Jonathan Daniel Ip,Anthony Chin-Ki Ng,Rosana W.S. Poon,Cui Ting Luo,Vincent C.C. Cheng,Jasper Fuk-Woo Chan,Jasper Fuk-Woo Chan,Ivan Hung,Zhiwei Chen,Honglin Chen,Kwok-Yung Yuen +25 more
TL;DR: The serial respiratory viral load of SARS-CoV-2 in posterior oropharyngeal saliva samples from patients with COVID-19, and serum antibody responses from patients infected with severe acute respiratory syndrome coronavirus 2 are ascertained.
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TL;DR: The findings provide strong empirical support for the routine application of serological testing in the diagnosis and management of COVID-19 patients and offer vital clinical information during the course of SARS-CoV-2 infection.
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Estimating the asymptomatic proportion of coronavirus disease 2019 (COVID-19) cases on board the Diamond Princess cruise ship, Yokohama, Japan, 2020.
Kenji Mizumoto,Kenji Mizumoto,Katsushi Kagaya,Katsushi Kagaya,Alexander E. Zarebski,Gerardo Chowell +5 more
TL;DR: On 5 February 2020, in Yokohama, Japan, a cruise ship hosting 3,711 people underwent a 2-week quarantine after a former passenger was found with COVID-19 post-disembarking, and the delay-adjusted asymptomatic proportion of infections, along with the infections’ timeline were derived.
Journal ArticleDOI
Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19)
Li Guo,Lili Ren,Siyuan Yang,Meng Xiao,De Chang,Fan Yang,Charles S. Dela Cruz,Yingying Wang,Chao Wu,Yan Xiao,Lulu Zhang,Lianlian Han,Shengyuan Dang,Yan Xu,Qi Wen Yang,Sheng Yong Xu,Hua Dong Zhu,Yingchun Xu,Qi Jin,Lokesh Sharma,Linghang Wang,Jianwei Wang +21 more
TL;DR: The humoral response to SARS-CoV-2 can aid in the diagnosis of COVID-19, including subclinical cases, and the detection efficiency by IgM ELISA is higher than that of qPCR after 5.5 days of symptom onset.
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