Journal ArticleDOI
Doublecortin expression levels in adult brain reflect neurogenesis.
Sebastien Couillard-Despres,Beate Winner,Susanne Schaubeck,Robert Aigner,Maurice Vroemen,Norbert Weidner,Ulrich Bogdahn,Jürgen Winkler,Hans-Georg Kuhn,Ludwig Aigner +9 more
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TLDR
It is demonstrated that quantification of DCX‐expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis, and DCX is a valuable alternative to techniques currently used to measure the levels of Neurogenesis.Abstract:
Progress in the field of neurogenesis is currently limited by the lack of tools enabling fast and quantitative analysis of neurogenesis in the adult brain Doublecortin (DCX) has recently been used as a marker for neurogenesis However, it was not clear whether DCX could be used to assess modulations occurring in the rate of neurogenesis in the adult mammalian central nervous system following lesioning or stimulatory factors Using two paradigms increasing neurogenesis levels (physical activity and epileptic seizures), we demonstrate that quantification of DCX-expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis Importantly, we excluded induction of DCX expression during physiological or reactive gliogenesis and excluded also DCX re-expression during regenerative axonal growth Our data validate DCX as a reliable and specific marker that reflects levels of adult neurogenesis and its modulation We demonstrate that DCX is a valuable alternative to techniques currently used to measure the levels of neurogenesis Importantly, in contrast to conventional techniques, analysis of neurogenesis through the detection of DCX does not require in vivo labelling of proliferating cells, thereby opening new avenues for the study of human neurogenesis under normal and pathological conditionsread more
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Dissertation
Hippocampal Neurogenesis in the Kindling Model of Temporal Lobe Epilepsy
TL;DR: Examination of changes in neurogenesis in the dentate gyrus in the kindling model of temporal lobe epilepsy in rats is examined and the potential impact of these changes on hippocampal function is explored.
Journal ArticleDOI
Effects of nerve growth factor in experimental model of focal microgyria.
Antonio Chiaretti,Alessandro Narducci,Federica Novegno,A. Antonelli,Filomena Pierri,Claudia Fantacci,Concezio Di Rocco,Gianpiero Tamburrini +7 more
TL;DR: NGF administration interferes with neural repair mechanisms at the polymicrogyric lesion site by means of TrkA and DCX upregulation which possibly counteracts the process of apoptosis caused by the brain injury.
Dissertation
Regulation of adult neurogenesis in Huntington's disease: The role of TGF-beta1 signaling in the neurogenic niche
TL;DR: Results reveal that TGF-beta1 signaling is virtually absent in proliferating stem cells but progressively active in post-mitotic neurons in the hippocampal stem cell niche of the healthy adult brain and appears to be a crucial modulator of neurogenesis in healthy and HD brains.
Journal ArticleDOI
Combined long-term enriched environment and caffeine supplementation improve memory function in C57Bl6 mice
TL;DR: In this article , the effects of caffeine supplementation in combination with prolonged physical and cognitive stimulation in the form of the enriched environment (EE) housing for a duration of 4 months were analyzed.
Expression of an anti-aggregant tau mutant (TauRD/ΔK280PP) is neuroprotective in organotypic hippocampal slice cultures
TL;DR: In this paper, transgenic hippocampal slice cultures were used to investigate the changes in the hippocampus of transgenic mice induced by overexpression of the anti-aggregant Tau repeat domain (TauRDΔ-PP).
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Journal ArticleDOI
Neurogenesis in the adult human hippocampus
Peter S. Eriksson,Ekaterina Perfilieva,Thomas Björk-Eriksson,Ann Marie Alborn,Claes Nordborg,Daniel A. Peterson,Fred H. Gage +6 more
TL;DR: It is demonstrated that new neurons, as defined by these markers, are generated from dividing progenitor cells in the dentate gyrus of adult humans, indicating that the human hippocampus retains its ability to generate neurons throughout life.
Journal ArticleDOI
Subventricular Zone Astrocytes Are Neural Stem Cells in the Adult Mammalian Brain
Fiona Doetsch,Isabelle Caillé,Daniel A. Lim,José Manuel García-Verdugo,Arturo Alvarez-Buylla +4 more
TL;DR: It is shown that SVZ astrocytes act as neural stem cells in both the normal and regenerating brain and give rise to cells that grow into multipotent neurospheres in vitro.
Journal ArticleDOI
Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus.
TL;DR: It is demonstrated that voluntary exercise is sufficient for enhanced neurogenesis in the adult mouse dentate gyrus, in amounts similar to enrichment conditions.
Journal ArticleDOI
Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats
Joseph Altman,Gopal D. Das +1 more
TL;DR: It is postulated that undifferentiated cells migrate postnatally from the forebrain ventricles to the hippocampus where they become differentiated, implicating that they may function as receptors of gonadal hormones.
Journal ArticleDOI
CNS stem cells express a new class of intermediate filament protein.
TL;DR: The predicted amino acid sequence of the nestin gene product shows that nestin defines a distinct sixth class of intermediate filament protein, extending a model in which transitions in intermediate filament gene expression reflect major steps in the pathway of neural differentiation.