Journal ArticleDOI
Doublecortin expression levels in adult brain reflect neurogenesis.
Sebastien Couillard-Despres,Beate Winner,Susanne Schaubeck,Robert Aigner,Maurice Vroemen,Norbert Weidner,Ulrich Bogdahn,Jürgen Winkler,Hans-Georg Kuhn,Ludwig Aigner +9 more
TLDR
It is demonstrated that quantification of DCX‐expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis, and DCX is a valuable alternative to techniques currently used to measure the levels of Neurogenesis.Abstract:
Progress in the field of neurogenesis is currently limited by the lack of tools enabling fast and quantitative analysis of neurogenesis in the adult brain Doublecortin (DCX) has recently been used as a marker for neurogenesis However, it was not clear whether DCX could be used to assess modulations occurring in the rate of neurogenesis in the adult mammalian central nervous system following lesioning or stimulatory factors Using two paradigms increasing neurogenesis levels (physical activity and epileptic seizures), we demonstrate that quantification of DCX-expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis Importantly, we excluded induction of DCX expression during physiological or reactive gliogenesis and excluded also DCX re-expression during regenerative axonal growth Our data validate DCX as a reliable and specific marker that reflects levels of adult neurogenesis and its modulation We demonstrate that DCX is a valuable alternative to techniques currently used to measure the levels of neurogenesis Importantly, in contrast to conventional techniques, analysis of neurogenesis through the detection of DCX does not require in vivo labelling of proliferating cells, thereby opening new avenues for the study of human neurogenesis under normal and pathological conditionsread more
Citations
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Journal ArticleDOI
In Vivo Monitoring of Adult Neurogenesis in Health and Disease
TL;DR: Progress is reviewed in the area of magnetic resonance imaging (MRI) and optical imaging and viral labeling of stem/progenitor cells and transgenic approaches, which have been used for in vivo imaging of neurogenesis.
Journal ArticleDOI
Doublecortin expression in CD8+ T-cells and microglia at sites of amyloid-β plaques: A potential role in shaping plaque pathology?
Michael S. Unger,Julia Marschallinger,Julia Marschallinger,Julia Kaindl,Barbara Klein,Mary Johnson,Ahmad Khundakar,Steffen Roßner,Michael T. Heneka,Sebastien Couillard-Despres,Edward Rockenstein,Eliezer Masliah,Johannes Attems,Ludwig Aigner +13 more
TL;DR: One characteristic of Alzheimer's disease is the formation of amyloid‐β plaques, which are typically linked to neuroinflammation and surrounded by inflammatory cells such as microglia and infiltrating immune cells.
Journal ArticleDOI
Mobilization of Neural Stem Cells and Generation of New Neurons in 6-OHDA–lesioned Rats by Intracerebroventricular Infusion of Liver Growth Factor
Rafael Gonzalo-Gobernado,Diana Reimers,Antonio S. Herranz,Juan J. Díaz-Gil,Cristina Osuna,María José Asensio,Silvia Baena,Macarena Rodríguez-Serrano,Eulalia Bazán +8 more
TL;DR: This study shows that LGF stimulates neurogenesis when applied intraventricularly in 6-hydroxydopamine–lesioned rats, and proposes LGF as a novel factor useful for neuronal replacement in neurodegenerative diseases.
Journal ArticleDOI
Regulation of adult neural precursor cell migration.
Soo Yuen Leong,Ann M. Turnley +1 more
TL;DR: The extrinsic factors and major intrinsic signalling pathways that regulate endogenous basal NPC migration to the olfactory bulb are highlighted and the role of inflammatory mediators and chemokines in disease and injury-induced NPC migration is highlighted.
Journal ArticleDOI
NeuroD modulates opioid agonist-selective regulation of adult neurogenesis and contextual memory extinction.
TL;DR: By controlling NeuroD activities, opioid agonists differentially regulate adult neurogenesis and subsequent contextual memory retention, such drug-related memory regulation could have implications in eventual context-associated relapse.
References
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Journal ArticleDOI
Neurogenesis in the adult human hippocampus
Peter S. Eriksson,Ekaterina Perfilieva,Thomas Björk-Eriksson,Ann Marie Alborn,Claes Nordborg,Daniel A. Peterson,Fred H. Gage +6 more
TL;DR: It is demonstrated that new neurons, as defined by these markers, are generated from dividing progenitor cells in the dentate gyrus of adult humans, indicating that the human hippocampus retains its ability to generate neurons throughout life.
Journal ArticleDOI
Subventricular Zone Astrocytes Are Neural Stem Cells in the Adult Mammalian Brain
Fiona Doetsch,Isabelle Caillé,Daniel A. Lim,José Manuel García-Verdugo,Arturo Alvarez-Buylla +4 more
TL;DR: It is shown that SVZ astrocytes act as neural stem cells in both the normal and regenerating brain and give rise to cells that grow into multipotent neurospheres in vitro.
Journal ArticleDOI
Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus.
TL;DR: It is demonstrated that voluntary exercise is sufficient for enhanced neurogenesis in the adult mouse dentate gyrus, in amounts similar to enrichment conditions.
Journal ArticleDOI
Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats
Joseph Altman,Gopal D. Das +1 more
TL;DR: It is postulated that undifferentiated cells migrate postnatally from the forebrain ventricles to the hippocampus where they become differentiated, implicating that they may function as receptors of gonadal hormones.
Journal ArticleDOI
CNS stem cells express a new class of intermediate filament protein.
TL;DR: The predicted amino acid sequence of the nestin gene product shows that nestin defines a distinct sixth class of intermediate filament protein, extending a model in which transitions in intermediate filament gene expression reflect major steps in the pathway of neural differentiation.