Journal ArticleDOI
Doublecortin expression levels in adult brain reflect neurogenesis.
Sebastien Couillard-Despres,Beate Winner,Susanne Schaubeck,Robert Aigner,Maurice Vroemen,Norbert Weidner,Ulrich Bogdahn,Jürgen Winkler,Hans-Georg Kuhn,Ludwig Aigner +9 more
TLDR
It is demonstrated that quantification of DCX‐expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis, and DCX is a valuable alternative to techniques currently used to measure the levels of Neurogenesis.Abstract:
Progress in the field of neurogenesis is currently limited by the lack of tools enabling fast and quantitative analysis of neurogenesis in the adult brain Doublecortin (DCX) has recently been used as a marker for neurogenesis However, it was not clear whether DCX could be used to assess modulations occurring in the rate of neurogenesis in the adult mammalian central nervous system following lesioning or stimulatory factors Using two paradigms increasing neurogenesis levels (physical activity and epileptic seizures), we demonstrate that quantification of DCX-expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis Importantly, we excluded induction of DCX expression during physiological or reactive gliogenesis and excluded also DCX re-expression during regenerative axonal growth Our data validate DCX as a reliable and specific marker that reflects levels of adult neurogenesis and its modulation We demonstrate that DCX is a valuable alternative to techniques currently used to measure the levels of neurogenesis Importantly, in contrast to conventional techniques, analysis of neurogenesis through the detection of DCX does not require in vivo labelling of proliferating cells, thereby opening new avenues for the study of human neurogenesis under normal and pathological conditionsread more
Citations
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Book ChapterDOI
Immunocytochemical Identification of Mammalian Differentiating Neurons in the Process of Adult Neurogenesis in the Hippocampal Dentate Gyrus
Book ChapterDOI
Nutrition, Macrobiotics, and the Brain’s Neuroinflammatory Response
TL;DR: Preclinical and clinical studies have revealed the anti-inflammatory properties of plant-derived compounds, such as polyphenols and polyunsaturated fatty acids, Nevertheless, no specific diets have been adopted in patients with multiple sclerosis (MS) or Alzheimer's or Parkinson’s diseases.
Journal ArticleDOI
Hyperbaric Oxygenation Prevents Loss of Immature Neurons in the Adult Hippocampal Dentate Gyrus Following Brain Injury
Rada Jeremic,Sanja Pekovic,Irena Lavrnja,Ivana Bjelobaba,Marina Djelic,Sanja Dacic,Predrag Brkic +6 more
TL;DR: In this article , the effects of sensorimotor cortex ablation (SCA) and hyperbaric oxygenation (HBO) treatment (HBOT) on the processes of neurogenesis in the adult dentate gyrus (DG), a region of the hippocampus that is the site of adult NSCs, were investigated.
Journal ArticleDOI
Different Neurogenic Potential in the Subnuclei of the Postnatal Rat Cochlear Nucleus.
Johannes Voelker,Jonas Engert,Christine Voelker,Linda Bieniussa,Philipp Schendzielorz,Rudolf Hagen,Kristen Rak +6 more
TL;DR: In this article, the authors showed that in all sub-nuclei of rat cochlear nucleus, there is a postnatal neural stem cell niche, which differs significantly in its potential The results can be explained by the origin from different regions in the rhombic lip, the species, and various analysis techniques applied.
Dissertation
Fate modulation of oligodendroglial progenitor cells and adult neural stem cells
TL;DR: The modulation mechanisms proposed here provide a basis for the development of future cell replacement strategies aiming at remyelination and show that the cell fate of oligodendroglial progenitor cells and adult neural stem cells can be modulated by both intrinsic and extrinsic regulation.
References
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Journal ArticleDOI
Neurogenesis in the adult human hippocampus
Peter S. Eriksson,Ekaterina Perfilieva,Thomas Björk-Eriksson,Ann Marie Alborn,Claes Nordborg,Daniel A. Peterson,Fred H. Gage +6 more
TL;DR: It is demonstrated that new neurons, as defined by these markers, are generated from dividing progenitor cells in the dentate gyrus of adult humans, indicating that the human hippocampus retains its ability to generate neurons throughout life.
Journal ArticleDOI
Subventricular Zone Astrocytes Are Neural Stem Cells in the Adult Mammalian Brain
Fiona Doetsch,Isabelle Caillé,Daniel A. Lim,José Manuel García-Verdugo,Arturo Alvarez-Buylla +4 more
TL;DR: It is shown that SVZ astrocytes act as neural stem cells in both the normal and regenerating brain and give rise to cells that grow into multipotent neurospheres in vitro.
Journal ArticleDOI
Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus.
TL;DR: It is demonstrated that voluntary exercise is sufficient for enhanced neurogenesis in the adult mouse dentate gyrus, in amounts similar to enrichment conditions.
Journal ArticleDOI
Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats
Joseph Altman,Gopal D. Das +1 more
TL;DR: It is postulated that undifferentiated cells migrate postnatally from the forebrain ventricles to the hippocampus where they become differentiated, implicating that they may function as receptors of gonadal hormones.
Journal ArticleDOI
CNS stem cells express a new class of intermediate filament protein.
TL;DR: The predicted amino acid sequence of the nestin gene product shows that nestin defines a distinct sixth class of intermediate filament protein, extending a model in which transitions in intermediate filament gene expression reflect major steps in the pathway of neural differentiation.