Journal ArticleDOI
Doublecortin expression levels in adult brain reflect neurogenesis.
Sebastien Couillard-Despres,Beate Winner,Susanne Schaubeck,Robert Aigner,Maurice Vroemen,Norbert Weidner,Ulrich Bogdahn,Jürgen Winkler,Hans-Georg Kuhn,Ludwig Aigner +9 more
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TLDR
It is demonstrated that quantification of DCX‐expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis, and DCX is a valuable alternative to techniques currently used to measure the levels of Neurogenesis.Abstract:
Progress in the field of neurogenesis is currently limited by the lack of tools enabling fast and quantitative analysis of neurogenesis in the adult brain Doublecortin (DCX) has recently been used as a marker for neurogenesis However, it was not clear whether DCX could be used to assess modulations occurring in the rate of neurogenesis in the adult mammalian central nervous system following lesioning or stimulatory factors Using two paradigms increasing neurogenesis levels (physical activity and epileptic seizures), we demonstrate that quantification of DCX-expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis Importantly, we excluded induction of DCX expression during physiological or reactive gliogenesis and excluded also DCX re-expression during regenerative axonal growth Our data validate DCX as a reliable and specific marker that reflects levels of adult neurogenesis and its modulation We demonstrate that DCX is a valuable alternative to techniques currently used to measure the levels of neurogenesis Importantly, in contrast to conventional techniques, analysis of neurogenesis through the detection of DCX does not require in vivo labelling of proliferating cells, thereby opening new avenues for the study of human neurogenesis under normal and pathological conditionsread more
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Journal ArticleDOI
Cdk6-dependent regulation of G(1) length controls adult neurogenesis.
Pierre Beukelaers,Renaud Vandenbosch,Nicolas Caron,Laurent Nguyen,Shibeshih Belachew,Gustave Moonen,Hiroaki Kiyokawa,Mariano Barbacid,David Santamaría,Brigitte Malgrange +9 more
TL;DR: The data support G1 length as an essential regulator of the switch between proliferation and neuronal differentiation in the adult brain and Cdk6 as one intrinsic key molecular regulator of this process.
Journal ArticleDOI
Analysis of dosing regimen and reproducibility of intraspinal grafting of human spinal stem cells in immunosuppressed minipigs.
Dusan Usvald,Peter Vodicka,Jana Hlučilová,Radek Prochazka,Jan Motlik,Karolina Kuchorova,Karolina Kuchorova,Karl Johe,Silvia Marsala,Miriam Scadeng,Osamu Kakinohana,Roman Navarro,Marian S̆anta,Michael P. Hefferan,Tony L. Yaksh,Martin Marsala,Martin Marsala +16 more
TL;DR: It is shown that highly reproducible delivery of a potential cell therapeutic candidate into spinal parenchyma can be achieved across a wide range of cell doses by direct intraspinal injections and that a modest motor weakness was observed in three of eight animals but was no longer present at 4 days to 7 weeks.
Journal ArticleDOI
Doublecortin-positive cells in the adult primate cerebral cortex and possible role in brain plasticity and development
Jocelyne Bloch,Mélanie Kaeser,Mélanie Kaeser,Yalda Sadeghi,Eric M. Rouiller,D. Eugene Redmond,Jean-François Brunet +6 more
TL;DR: The expression of doublecortin (DCX) in cells based on reverse‐transcription polymerase chain reaction (RT‐PCR) and immunodetection in the adult primate cortex and cell cultures showed that DCX‐positive cells were present in the whole primate cerebral cortex and also expressed glial and/or neuronal markers.
Journal ArticleDOI
Acute and Fractionated Exposure to High-LET 56Fe HZE-Particle Radiation Both Result in Similar Long-Term Deficits in Adult Hippocampal Neurogenesis
Phillip D. Rivera,Hung Ying Shih,Junie A. Leblanc,Mara G. Cole,Wellington Z. Amaral,Shibani Mukherjee,Shichuan Zhang,Melanie J. Lucero,Nathan A. DeCarolis,Benjamin P C Chen,Amelia J. Eisch +10 more
TL;DR: It is shown that single and fractionated exposures of 56Fe-particle irradiation are similarly detrimental to adult-generated neurons, and Implications for future missions and ground-based studies in space radiation are discussed.
Journal ArticleDOI
Impact of social status and antidepressant treatment on neurogenesis in the baboon hippocampus.
Melody V. Wu,Jul Lea Shamy,Gillinder Bedi,Chien-Wen J Choi,Melanie M. Wall,Victoria Arango,Maura Boldrini,Richard W. Foltin,René Hen +8 more
TL;DR: These findings are the first to indicate that adult neurogenesis in the baboon hippocampal DG may be functionally relevant in the context of social stress and mechanisms of antidepressant action.
References
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Journal ArticleDOI
Neurogenesis in the adult human hippocampus
Peter S. Eriksson,Ekaterina Perfilieva,Thomas Björk-Eriksson,Ann Marie Alborn,Claes Nordborg,Daniel A. Peterson,Fred H. Gage +6 more
TL;DR: It is demonstrated that new neurons, as defined by these markers, are generated from dividing progenitor cells in the dentate gyrus of adult humans, indicating that the human hippocampus retains its ability to generate neurons throughout life.
Journal ArticleDOI
Subventricular Zone Astrocytes Are Neural Stem Cells in the Adult Mammalian Brain
Fiona Doetsch,Isabelle Caillé,Daniel A. Lim,José Manuel García-Verdugo,Arturo Alvarez-Buylla +4 more
TL;DR: It is shown that SVZ astrocytes act as neural stem cells in both the normal and regenerating brain and give rise to cells that grow into multipotent neurospheres in vitro.
Journal ArticleDOI
Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus.
TL;DR: It is demonstrated that voluntary exercise is sufficient for enhanced neurogenesis in the adult mouse dentate gyrus, in amounts similar to enrichment conditions.
Journal ArticleDOI
Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats
Joseph Altman,Gopal D. Das +1 more
TL;DR: It is postulated that undifferentiated cells migrate postnatally from the forebrain ventricles to the hippocampus where they become differentiated, implicating that they may function as receptors of gonadal hormones.
Journal ArticleDOI
CNS stem cells express a new class of intermediate filament protein.
TL;DR: The predicted amino acid sequence of the nestin gene product shows that nestin defines a distinct sixth class of intermediate filament protein, extending a model in which transitions in intermediate filament gene expression reflect major steps in the pathway of neural differentiation.