Journal ArticleDOI
Doublecortin expression levels in adult brain reflect neurogenesis.
Sebastien Couillard-Despres,Beate Winner,Susanne Schaubeck,Robert Aigner,Maurice Vroemen,Norbert Weidner,Ulrich Bogdahn,Jürgen Winkler,Hans-Georg Kuhn,Ludwig Aigner +9 more
TLDR
It is demonstrated that quantification of DCX‐expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis, and DCX is a valuable alternative to techniques currently used to measure the levels of Neurogenesis.Abstract:
Progress in the field of neurogenesis is currently limited by the lack of tools enabling fast and quantitative analysis of neurogenesis in the adult brain Doublecortin (DCX) has recently been used as a marker for neurogenesis However, it was not clear whether DCX could be used to assess modulations occurring in the rate of neurogenesis in the adult mammalian central nervous system following lesioning or stimulatory factors Using two paradigms increasing neurogenesis levels (physical activity and epileptic seizures), we demonstrate that quantification of DCX-expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis Importantly, we excluded induction of DCX expression during physiological or reactive gliogenesis and excluded also DCX re-expression during regenerative axonal growth Our data validate DCX as a reliable and specific marker that reflects levels of adult neurogenesis and its modulation We demonstrate that DCX is a valuable alternative to techniques currently used to measure the levels of neurogenesis Importantly, in contrast to conventional techniques, analysis of neurogenesis through the detection of DCX does not require in vivo labelling of proliferating cells, thereby opening new avenues for the study of human neurogenesis under normal and pathological conditionsread more
Citations
More filters
Journal ArticleDOI
Electroconvulsive therapy induces neurogenesis in frontal rat brain areas.
Dragos Inta,Juan M. Lima-Ojeda,Thorsten Lau,Wannan Tang,Christof Dormann,Rolf Sprengel,Patrick Schloss,Alexander Sartorius,Andreas Meyer-Lindenberg,Peter Gass +9 more
TL;DR: It is shown that ECT also strongly enhances neurogenesis in frontal brain areas, especially in the rostro-medial striatum, generating specific, small-size calretinin-positive interneurons.
Journal ArticleDOI
Suppression of Adult Neurogenesis Increases the Acute Effects of Kainic Acid
Sloka S. Iyengar,John LaFrancois,Daniel Friedman,Liam Drew,Christine A. Denny,Nesha S. Burghardt,Melody V. Wu,Jenny Hsieh,René Hen,Helen E. Scharfman,Helen E. Scharfman +10 more
TL;DR: The hypothesis that reduction of adult-born neurons increases the susceptibility of the brain to effects of KA is supported.
Journal ArticleDOI
Age-specific effects of voluntary exercise on memory and the older brain.
Joyce Siette,Joyce Siette,R. Frederick Westbrook,Carl W. Cotman,Kuldip S. Sidhu,Wanlin Zhu,Perminder S. Sachdev,Michael Valenzuela,Michael Valenzuela +8 more
TL;DR: It is revealed that voluntary running completely reverses the profound degradation of hippocampal network efficiency that accompanies sedentary aging and at an individual animal level, both overall hippocampal presynaptic density and subregional connectivity independently contribute to prediction of successful place recognition memory performance.
Journal ArticleDOI
Neurogenesis in the human hippocampus and its relevance to temporal lobe epilepsies
TL;DR: Surgical specimens obtained from TLE patients represent an important tool to study mechanisms of stem cell recruitment, proliferation and differentiation in the human brain, as well as opening new avenues to systematically explore disease pathomechanisms in chronic epilepsies.
Journal ArticleDOI
Modifications of Hippocampal Circuits and Early Disruption of Adult Neurogenesis in the Tg2576 Mouse Model of Alzheimer’s Disease
Alice Krezymon,Kevin Richetin,Kevin Richetin,Hélène Halley,Hélène Halley,Laurent Roybon,Jean-Michel Lassalle,Jean-Michel Lassalle,Bernard Frances,Bernard Frances,Laure Verret,Laure Verret,Claire Rampon,Claire Rampon +13 more
TL;DR: Disruption of adult hippocampal neurogenesis occurred before network remodeling in this mouse model and therefore may account as an early event in the etiology of Alzheimer’s pathology.
References
More filters
Journal ArticleDOI
Neurogenesis in the adult human hippocampus
Peter S. Eriksson,Ekaterina Perfilieva,Thomas Björk-Eriksson,Ann Marie Alborn,Claes Nordborg,Daniel A. Peterson,Fred H. Gage +6 more
TL;DR: It is demonstrated that new neurons, as defined by these markers, are generated from dividing progenitor cells in the dentate gyrus of adult humans, indicating that the human hippocampus retains its ability to generate neurons throughout life.
Journal ArticleDOI
Subventricular Zone Astrocytes Are Neural Stem Cells in the Adult Mammalian Brain
Fiona Doetsch,Isabelle Caillé,Daniel A. Lim,José Manuel García-Verdugo,Arturo Alvarez-Buylla +4 more
TL;DR: It is shown that SVZ astrocytes act as neural stem cells in both the normal and regenerating brain and give rise to cells that grow into multipotent neurospheres in vitro.
Journal ArticleDOI
Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus.
TL;DR: It is demonstrated that voluntary exercise is sufficient for enhanced neurogenesis in the adult mouse dentate gyrus, in amounts similar to enrichment conditions.
Journal ArticleDOI
Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats
Joseph Altman,Gopal D. Das +1 more
TL;DR: It is postulated that undifferentiated cells migrate postnatally from the forebrain ventricles to the hippocampus where they become differentiated, implicating that they may function as receptors of gonadal hormones.
Journal ArticleDOI
CNS stem cells express a new class of intermediate filament protein.
TL;DR: The predicted amino acid sequence of the nestin gene product shows that nestin defines a distinct sixth class of intermediate filament protein, extending a model in which transitions in intermediate filament gene expression reflect major steps in the pathway of neural differentiation.