Journal ArticleDOI
Doublecortin expression levels in adult brain reflect neurogenesis.
Sebastien Couillard-Despres,Beate Winner,Susanne Schaubeck,Robert Aigner,Maurice Vroemen,Norbert Weidner,Ulrich Bogdahn,Jürgen Winkler,Hans-Georg Kuhn,Ludwig Aigner +9 more
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TLDR
It is demonstrated that quantification of DCX‐expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis, and DCX is a valuable alternative to techniques currently used to measure the levels of Neurogenesis.Abstract:
Progress in the field of neurogenesis is currently limited by the lack of tools enabling fast and quantitative analysis of neurogenesis in the adult brain Doublecortin (DCX) has recently been used as a marker for neurogenesis However, it was not clear whether DCX could be used to assess modulations occurring in the rate of neurogenesis in the adult mammalian central nervous system following lesioning or stimulatory factors Using two paradigms increasing neurogenesis levels (physical activity and epileptic seizures), we demonstrate that quantification of DCX-expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis Importantly, we excluded induction of DCX expression during physiological or reactive gliogenesis and excluded also DCX re-expression during regenerative axonal growth Our data validate DCX as a reliable and specific marker that reflects levels of adult neurogenesis and its modulation We demonstrate that DCX is a valuable alternative to techniques currently used to measure the levels of neurogenesis Importantly, in contrast to conventional techniques, analysis of neurogenesis through the detection of DCX does not require in vivo labelling of proliferating cells, thereby opening new avenues for the study of human neurogenesis under normal and pathological conditionsread more
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The Combination of Human Urinary Kallidinogenase and Mild Hypothermia Protects Adult Rats Against Hypoxic-Ischemic Encephalopathy-Induced Injury by Promoting Angiogenesis and Regeneration.
Xiaoya Gao,Haiting Xie,Shuzhen Zhu,Bin Yu,Ying Xian,Qian Ouyang,Yabin Ji,Xiaohua Yang,Chunyan Wen,Penghua Wang,Yufeng Tong,Qing Wang +11 more
TL;DR: It is demonstrated that both H UK and MH are neuroprotective after HIE insult; however, the combined therapy with HUK and MH enhanced the efficiency and efficacy of either therapy alone in the treatment of HIE, at least partially by promoting angiogenesis and regeneration and rescuing tight-junction loss.
Journal ArticleDOI
Expression of doublecortin in tumours of the central and peripheral nervous system and in human non-neuronal tissues.
TL;DR: Doublecortin can be regarded as specific neuronal marker only in normal developing brain, but lacks specificity in glioneuronal and glial tumours and other non-neuronal human tissues where it is expressed in a wide variety of tumour tissue.
Journal ArticleDOI
Differential roles of exogenous protein disulfide isomerase A3 on proliferating cell and neuroblast numbers in the normal and ischemic gerbils
Dae Young Yoo,Dae Young Yoo,Su Bin Cho,Hyo Young Jung,Woosuk Kim,Sung Min Nam,Jong Whi Kim,Seung Myung Moon,Yeo Sung Yoon,Dae Won Kim,Soo Young Choi,In Koo Hwang +11 more
TL;DR: The effects of exogenous protein disulfide isomerase A3 (PDIA3) on hippocampal neurogenesis in gerbils under control and ischemic damage are examined.
Journal ArticleDOI
Genetic Ablation of Neural Progenitor Cells Impairs Acquisition of Trace Eyeblink Conditioning.
TL;DR: The results are consistent with, and expand upon, prior studies that demonstrated that adult-born neurons are involved in the formation of associative memories.
Journal ArticleDOI
Effects of Cu,Zn-superoxide dismutase on cell proliferation and neuroblast differentiation in the mouse dentate gyrus.
Dae Young Yoo,Bich Na Shin,In Hye Kim,Woosuk Kim,Dae Won Kim,Ki-Yeon Yoo,Jung Hoon Choi,Choong Hyun Lee,Yeo Sung Yoon,Soo Young Choi,Moo Ho Won,In Koo Hwang +11 more
TL;DR: The results suggest that a SOD1 supplement to healthy mice may not be necessary to modulate cell proliferation and neuroblast differentiation in the dentate gyrus.
References
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Journal ArticleDOI
Neurogenesis in the adult human hippocampus
Peter S. Eriksson,Ekaterina Perfilieva,Thomas Björk-Eriksson,Ann Marie Alborn,Claes Nordborg,Daniel A. Peterson,Fred H. Gage +6 more
TL;DR: It is demonstrated that new neurons, as defined by these markers, are generated from dividing progenitor cells in the dentate gyrus of adult humans, indicating that the human hippocampus retains its ability to generate neurons throughout life.
Journal ArticleDOI
Subventricular Zone Astrocytes Are Neural Stem Cells in the Adult Mammalian Brain
Fiona Doetsch,Isabelle Caillé,Daniel A. Lim,José Manuel García-Verdugo,Arturo Alvarez-Buylla +4 more
TL;DR: It is shown that SVZ astrocytes act as neural stem cells in both the normal and regenerating brain and give rise to cells that grow into multipotent neurospheres in vitro.
Journal ArticleDOI
Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus.
TL;DR: It is demonstrated that voluntary exercise is sufficient for enhanced neurogenesis in the adult mouse dentate gyrus, in amounts similar to enrichment conditions.
Journal ArticleDOI
Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats
Joseph Altman,Gopal D. Das +1 more
TL;DR: It is postulated that undifferentiated cells migrate postnatally from the forebrain ventricles to the hippocampus where they become differentiated, implicating that they may function as receptors of gonadal hormones.
Journal ArticleDOI
CNS stem cells express a new class of intermediate filament protein.
TL;DR: The predicted amino acid sequence of the nestin gene product shows that nestin defines a distinct sixth class of intermediate filament protein, extending a model in which transitions in intermediate filament gene expression reflect major steps in the pathway of neural differentiation.