Journal ArticleDOI
Doublecortin expression levels in adult brain reflect neurogenesis.
Sebastien Couillard-Despres,Beate Winner,Susanne Schaubeck,Robert Aigner,Maurice Vroemen,Norbert Weidner,Ulrich Bogdahn,Jürgen Winkler,Hans-Georg Kuhn,Ludwig Aigner +9 more
Reads0
Chats0
TLDR
It is demonstrated that quantification of DCX‐expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis, and DCX is a valuable alternative to techniques currently used to measure the levels of Neurogenesis.Abstract:
Progress in the field of neurogenesis is currently limited by the lack of tools enabling fast and quantitative analysis of neurogenesis in the adult brain Doublecortin (DCX) has recently been used as a marker for neurogenesis However, it was not clear whether DCX could be used to assess modulations occurring in the rate of neurogenesis in the adult mammalian central nervous system following lesioning or stimulatory factors Using two paradigms increasing neurogenesis levels (physical activity and epileptic seizures), we demonstrate that quantification of DCX-expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis Importantly, we excluded induction of DCX expression during physiological or reactive gliogenesis and excluded also DCX re-expression during regenerative axonal growth Our data validate DCX as a reliable and specific marker that reflects levels of adult neurogenesis and its modulation We demonstrate that DCX is a valuable alternative to techniques currently used to measure the levels of neurogenesis Importantly, in contrast to conventional techniques, analysis of neurogenesis through the detection of DCX does not require in vivo labelling of proliferating cells, thereby opening new avenues for the study of human neurogenesis under normal and pathological conditionsread more
Citations
More filters
Journal ArticleDOI
Microbats appear to have adult hippocampal neurogenesis, but post-capture stress causes a rapid decline in the number of neurons expressing doublecortin.
Richard Chawana,Abdulaziz N. Alagaili,Nina Patzke,Muhammad A. Spocter,Muhammad A. Spocter,Osama B. Mohammed,Consolate Kaswera,Emmanuel Gilissen,Emmanuel Gilissen,Emmanuel Gilissen,Nigel C. Bennett,Amadi O. Ihunwo,Paul R. Manger +12 more
TL;DR: Bats, including both microchiropteran bats and megachiropterans, appear to exhibit substantial levels of adult hippocampal neurogenesis, as with all other mammals studied to date apart from cetaceans.
Journal ArticleDOI
Melatonin Reverses the Depression-associated Behaviour and Regulates Microglia, Fractalkine Expression and Neurogenesis in Adult Mice Exposed to Chronic Mild Stress
Nelly Maritza Vega-Rivera,Leonardo Ortiz-López,Andrea Granados-Juárez,Erika Monserrat Estrada-Camarena,Gerardo Ramírez-Rodríguez +4 more
TL;DR: The outcome of this study is consistent with the hypothesis about the antidepressant-like effect of melatonin and supports its relevance as a modulator of the niche in the dentate gyrus.
Journal ArticleDOI
Potential neuronal repair in cerebral white matter injury in the human neonate.
Robin L. Haynes,Gang Xu,Rebecca D. Folkerth,Felicia L. Trachtenberg,Joseph J. Volpe,Hannah C. Kinney +5 more
TL;DR: The hypothesis that intrinsic repair occurs in PVL to attempt to compensate for the deficits in white matter neurons is tested and evidence suggestive of an attempt at neuronal repair or regeneration in human neonatal white matter injury is provided.
Journal ArticleDOI
Combination Effects of Sodium Butyrate and Pyridoxine Treatment on Cell Proliferation and Neuroblast Differentiation in the Dentate Gyrus of d-Galactose-Induced Aging Model Mice
Dae Young Yoo,Woosuk Kim,In Hye Kim,Sung Min Nam,Jin Young Chung,Jung Hoon Choi,Yeo Sung Yoon,Moo Ho Won,In Koo Hwang +8 more
TL;DR: Results indicate that the combination treatment of Pyr with SB in d-gal mice ameliorated the d-Gal-induced reduction in cell proliferation, neuroblast differentiation, and memory deficits.
Journal ArticleDOI
Effects of [Nphe1, Arg14, Lys15] N/OFQ-NH2 (UFP-101), a potent NOP receptor antagonist, on molecular, cellular and behavioural alterations associated with chronic mild stress:
Giovanni Vitale,Monica Filaferro,Maria Vittoria Micioni Di Bonaventura,Valentina Ruggieri,Carlo Cifani,Remo Guerrini,Michele Simonato,Silvia Zucchini +7 more
TL;DR: The view that blockade of NOP receptors produces antidepressant-like effects in CMS associated with positive effects on neurogenesis and FGF-2 expression is supported, therefore, NOP receptor receptors may represent a target for innovative antidepressant drugs.
References
More filters
Journal ArticleDOI
Neurogenesis in the adult human hippocampus
Peter S. Eriksson,Ekaterina Perfilieva,Thomas Björk-Eriksson,Ann Marie Alborn,Claes Nordborg,Daniel A. Peterson,Fred H. Gage +6 more
TL;DR: It is demonstrated that new neurons, as defined by these markers, are generated from dividing progenitor cells in the dentate gyrus of adult humans, indicating that the human hippocampus retains its ability to generate neurons throughout life.
Journal ArticleDOI
Subventricular Zone Astrocytes Are Neural Stem Cells in the Adult Mammalian Brain
Fiona Doetsch,Isabelle Caillé,Daniel A. Lim,José Manuel García-Verdugo,Arturo Alvarez-Buylla +4 more
TL;DR: It is shown that SVZ astrocytes act as neural stem cells in both the normal and regenerating brain and give rise to cells that grow into multipotent neurospheres in vitro.
Journal ArticleDOI
Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus.
TL;DR: It is demonstrated that voluntary exercise is sufficient for enhanced neurogenesis in the adult mouse dentate gyrus, in amounts similar to enrichment conditions.
Journal ArticleDOI
Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats
Joseph Altman,Gopal D. Das +1 more
TL;DR: It is postulated that undifferentiated cells migrate postnatally from the forebrain ventricles to the hippocampus where they become differentiated, implicating that they may function as receptors of gonadal hormones.
Journal ArticleDOI
CNS stem cells express a new class of intermediate filament protein.
TL;DR: The predicted amino acid sequence of the nestin gene product shows that nestin defines a distinct sixth class of intermediate filament protein, extending a model in which transitions in intermediate filament gene expression reflect major steps in the pathway of neural differentiation.