Journal ArticleDOI
Doublecortin expression levels in adult brain reflect neurogenesis.
Sebastien Couillard-Despres,Beate Winner,Susanne Schaubeck,Robert Aigner,Maurice Vroemen,Norbert Weidner,Ulrich Bogdahn,Jürgen Winkler,Hans-Georg Kuhn,Ludwig Aigner +9 more
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TLDR
It is demonstrated that quantification of DCX‐expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis, and DCX is a valuable alternative to techniques currently used to measure the levels of Neurogenesis.Abstract:
Progress in the field of neurogenesis is currently limited by the lack of tools enabling fast and quantitative analysis of neurogenesis in the adult brain Doublecortin (DCX) has recently been used as a marker for neurogenesis However, it was not clear whether DCX could be used to assess modulations occurring in the rate of neurogenesis in the adult mammalian central nervous system following lesioning or stimulatory factors Using two paradigms increasing neurogenesis levels (physical activity and epileptic seizures), we demonstrate that quantification of DCX-expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis Importantly, we excluded induction of DCX expression during physiological or reactive gliogenesis and excluded also DCX re-expression during regenerative axonal growth Our data validate DCX as a reliable and specific marker that reflects levels of adult neurogenesis and its modulation We demonstrate that DCX is a valuable alternative to techniques currently used to measure the levels of neurogenesis Importantly, in contrast to conventional techniques, analysis of neurogenesis through the detection of DCX does not require in vivo labelling of proliferating cells, thereby opening new avenues for the study of human neurogenesis under normal and pathological conditionsread more
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Nestin- and doublecortin-positive cells reside in adult spinal cord meninges and participate in injury-induced parenchymal reaction.
Ilaria Decimo,Francesco Bifari,Francisco Rodríguez,Giorgio Malpeli,Sissi Dolci,Valentina Lavarini,Silvia Pretto,Sandra Vasquez,Marina Sciancalepore,Alberto Montalbano,Valeria Berton,Mauro Krampera,Guido Francesco Fumagalli +12 more
TL;DR: It is shown that meningeal cells, including nestin‐ and doublecortin‐positive cells, migrate in the spinal cord parenchyma and contribute to the glial scar formation, indicating for the first time that spinal cord meninges are potential niches harboring stem/precursor cells that can be activated by injury.
Journal ArticleDOI
Regulation of Stroke-Induced Neurogenesis in Adult Brain—Recent Scientific Progress
TL;DR: Recent findings on the regulation of neurogenesis after stroke are described, in particular regarding the duration of the neurogenic response and the influence of age, as well as the molecular mechanisms influencing migration and survival of the new neurons.
Journal ArticleDOI
Cancer stem cell contribution to glioblastoma invasiveness
TL;DR: Recent findings demonstrating the invasive nature of GBM cancer stem cells, together with novel candidate molecules associated with both cancer stem cell biology and GBM invasion, like doublecortin and microRNAs are reviewed to affect the design of effective therapies currently not considered for GBM invasive progression.
Journal ArticleDOI
Transcranial low-level laser therapy enhances learning, memory, and neuroprogenitor cells after traumatic brain injury in mice
TL;DR: The study results suggest that tLLLT may improve TBI both by reducing cell death in the lesion and by stimulating neurogenesis.
Journal ArticleDOI
Neural plasticity and stress coping in teleost fishes.
TL;DR: The role of an important contributor to neural processing, the set of biochemical, molecular, and structural processes collectively referred to as neural plasticity, is reviewed, focusing on work in teleost fishes, while also elucidating conserved aspects.
References
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Journal ArticleDOI
Neurogenesis in the adult human hippocampus
Peter S. Eriksson,Ekaterina Perfilieva,Thomas Björk-Eriksson,Ann Marie Alborn,Claes Nordborg,Daniel A. Peterson,Fred H. Gage +6 more
TL;DR: It is demonstrated that new neurons, as defined by these markers, are generated from dividing progenitor cells in the dentate gyrus of adult humans, indicating that the human hippocampus retains its ability to generate neurons throughout life.
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Subventricular Zone Astrocytes Are Neural Stem Cells in the Adult Mammalian Brain
Fiona Doetsch,Isabelle Caillé,Daniel A. Lim,José Manuel García-Verdugo,Arturo Alvarez-Buylla +4 more
TL;DR: It is shown that SVZ astrocytes act as neural stem cells in both the normal and regenerating brain and give rise to cells that grow into multipotent neurospheres in vitro.
Journal ArticleDOI
Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus.
TL;DR: It is demonstrated that voluntary exercise is sufficient for enhanced neurogenesis in the adult mouse dentate gyrus, in amounts similar to enrichment conditions.
Journal ArticleDOI
Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats
Joseph Altman,Gopal D. Das +1 more
TL;DR: It is postulated that undifferentiated cells migrate postnatally from the forebrain ventricles to the hippocampus where they become differentiated, implicating that they may function as receptors of gonadal hormones.
Journal ArticleDOI
CNS stem cells express a new class of intermediate filament protein.
TL;DR: The predicted amino acid sequence of the nestin gene product shows that nestin defines a distinct sixth class of intermediate filament protein, extending a model in which transitions in intermediate filament gene expression reflect major steps in the pathway of neural differentiation.