Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis.
TLDR
This review covers the well-recognized clathrin-mediated endocytosis (CME), phagocytotic, and macropinocyTosis in addition to the less addressed pathways that take place in lipid rafts, and summarizes the regulators associated with each uptake pathway.About:
This article is published in Molecular Therapy.The article was published on 2013-06-01 and is currently open access. It has received 247 citations till now. The article focuses on the topics: Endocytic vesicle & Endocytic cycle.read more
Citations
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An endosomal LAPF is required for macrophage endocytosis and elimination of bacteria
TL;DR: This study shows that a Cav1-interacting protein, LAPF, promotes the endocytosis of bacteria and production of proinflammatory cytokines by inducing Src-LAPF-Caveolin complex formation in response to innate stimuli and proposes that pharmacological activation of LAPf and Src could potentially be applied to the control of bacterial infections.
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A novel paperclip double-stranded RNA structure demonstrates clathrin-independent uptake in the mosquito Aedes aegypti.
TL;DR: This study used pharmacological inhibitors and RNAi-mediated knockdown of endocytic genes to provide evidence that CME is the primary means of dsRNA uptake in the mosquito Aedes aegypti and observed that short "paperclip" RNAs (pcRNAs), with partially closed ends, efficiently enter cells via a clathrin-independent pathway and effectively facilitate transcript knockdown.
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Imaging macropinosomes during Shigella infections.
TL;DR: The combined imaging techniques introduced here provide a detailed characterization of macropinosomes during bacterial entry, which, apart from Shigella, are relevant for numerous other ones, including Salmonella, Brucella or Mycobacteria.
Journal ArticleDOI
Delaminated layered double hydroxide delivers DNA molecules as sandwich nanostructure into cells via a non-endocytic pathway
Ye Li,Wenlong Bao,Hongyang Wu,Junya Wang,Yu Zhang,Yinglang Wan,Dapeng Cao,Dermot O'Hare,Qiang Wang +8 more
TL;DR: It is experimentally found that LDH can efficiently carry DNA into the nucleus of cell in BY-2 suspension cells and the DPD simulations indicated that only the DNA-LDH-DNA sandwich structure could penetrate the plasma membrane.
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Pharmacological inhibition of mTORC1 increases CCKBR-specific tumor uptake of radiolabeled minigastrin analogue [ 177 Lu]Lu-PP-F11N
Michal Grzmil,Yun Qin,Carina Schleuniger,Stephan Frank,Stefan Imobersteg,Alain Blanc,Martin Spillmann,Philipp Berger,Roger Schibli,Roger Schibli,Martin Béhé +10 more
TL;DR: It is demonstrated that increased CCKBR protein level by RAD001 pretreatment has the potential to improve tumor uptake of [177Lu]Lu-PP-F11N and provides proof-of-concept for the development of molecular strategies aimed at enhancing the level of the targeted receptor, to increase the efficacy of PRRT and nuclear imaging.
References
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Journal ArticleDOI
Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
Journal ArticleDOI
The fluid mosaic model of the structure of cell membranes.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.
Journal ArticleDOI
Regulated portals of entry into the cell
Sean D. Conner,Sandra L. Schmid +1 more
TL;DR: ‘Endocytosis’ encompasses several diverse mechanisms by which cells internalize macromolecules and particles into transport vesicles derived from the plasma membrane and must be viewed in a broader context than simple vesicular trafficking.
Journal ArticleDOI
Mechanisms of Endocytosis
TL;DR: What is known about mammalian endocytic mechanisms is reviewed, with focus on the cellular proteins that control these events, and the functional relevance of distinctendocytic pathways is discussed.
Book ChapterDOI
THE FLUID MOSAIC MODEL OF THE STRUCTURE OF CELL MEMBRANES Reprinted with permission from Science, Copyright AAA, 18 February 1972, Volume 175, pp. 720–731.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.