Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis.
TLDR
This review covers the well-recognized clathrin-mediated endocytosis (CME), phagocytotic, and macropinocyTosis in addition to the less addressed pathways that take place in lipid rafts, and summarizes the regulators associated with each uptake pathway.About:
This article is published in Molecular Therapy.The article was published on 2013-06-01 and is currently open access. It has received 247 citations till now. The article focuses on the topics: Endocytic vesicle & Endocytic cycle.read more
Citations
More filters
Journal Article
Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes
Joanna Rejman,Massimo Conese +1 more
TL;DR: In this article, the authors investigated the effects of inhibitors of clathrin-mediated endocytosis (chlorpromazine and K(+) depletion) and of caveolae-mediated uptake (filipin and genistein) on internalization of FITC-poly-l-lysine-labeled DOTAP/DNA lipoplexes and PEI/DNA polyplexes by A549 pneumocytes and HeLa cells and on the transfection efficiencies of these complexes with the luciferase gene.
Journal ArticleDOI
Cellular uptake and trafficking of antisense oligonucleotides
TL;DR: Although PS-ASOs function in both the cytoplasm and nucleus, localization to different subcellular regions can affect their therapeutic potency and the main proteins involved in these processes have been identified and intracellular sites in which PS ASOs are active, or inactive, cataloged.
Journal ArticleDOI
Overcoming Nonviral Gene Delivery Barriers: Perspective and Future
TL;DR: This work presents the existing delivery barriers and summarize current vector-specific strategies to overcome said barriers, and focuses on the gene delivery barriers associated with the two most common classes of nonviral vectors, cationic-based lipids and polymers.
Journal ArticleDOI
Nonviral cancer gene therapy: Delivery cascade and vector nanoproperty integration.
Zhuxian Zhou,Xiangrui Liu,Dingcheng Zhu,Yue Wang,Zhen Zhang,Xuefei Zhou,Nasha Qiu,Xuesi Chen,Youqing Shen +8 more
TL;DR: This review analyzes the cancer gene‐delivery cascade and the barriers, the needed nanopro properties and the current strategies for overcoming these barriers, and outlines PEGylation, surface‐charge, size, and stability dilemmas in vector nanoproperties to efficiently accomplish the cancer genes delivery cascade.
Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells
Mark T. Howes,Matthew Kirkham,James D. Riches,Katia Cortese,Piers J. Walser,Fiona Simpson,Michelle M. Hill,Alun Jones,Richard Lundmark,Margaret R. Lindsay,Delia J. Hernández-Deviez,Gordana Hadzic,Adam McCluskey,Rumasia Bashir,Libin Liu,Paul F. Pilch,Harvey T. McMahon,Phillip J. Robinson,John F. Hancock,Satyajit Mayor,Robert G. Parton +20 more
TL;DR: In this article, the authors provided the first quantitative ultrastructural analysis and molecular characterization of the major endocytic pathway in fibroblasts, a pathway that provides rapid membrane turnover at the leading edge of migrating cells.
References
More filters
Journal ArticleDOI
Virus Entry by Endocytosis
TL;DR: This review focuses on the cell biology of virus entry and the different strategies and endocytic mechanisms used by animal viruses.
Journal ArticleDOI
Rapid endocytosis of interleukin 2 receptors when clathrin-coated pit endocytosis is inhibited
TL;DR: A model for receptor-mediated endocytosis is proposed that may account for results and published data on other receptors that suggest this cytokine and its receptors can still be rapidly endedocytosed in the absence of functional clathrin-coated structures.
Journal ArticleDOI
Endocytosis and retrograde transport of Shiga toxin
TL;DR: New discoveries concerning endocytosis and retrograde transport of Shiga toxin to the Golgi, the ER and the cytosol are focused on.
Journal ArticleDOI
Distinct mechanisms of clathrin-independent endocytosis have unique sphingolipid requirements.
Zhi-Jie Cheng,Raman Deep Singh,Deepak Sharma,Eileen L. Holicky,Kentaro Hanada,David L. Marks,Richard E. Pagano +6 more
TL;DR: Both the in vivo membrane targeting and in vitro binding to artificial lipid vesicles of RhoA and Cdc42 were shown to be dependent upon sphingomyelin, the first evidence that SLs are differentially required for distinct mechanisms of clathrin-independent endocytosis.
Journal ArticleDOI
Cortactin and dynamin are required for the clathrin-independent endocytosis of γc cytokine receptor
TL;DR: The results reveal the ubiquitous action of cortactin in internalization processes and suggest its role as a linker between actin dynamics and clathrin-dependent and -independent endocytosis.