Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis.
TLDR
This review covers the well-recognized clathrin-mediated endocytosis (CME), phagocytotic, and macropinocyTosis in addition to the less addressed pathways that take place in lipid rafts, and summarizes the regulators associated with each uptake pathway.About:
This article is published in Molecular Therapy.The article was published on 2013-06-01 and is currently open access. It has received 247 citations till now. The article focuses on the topics: Endocytic vesicle & Endocytic cycle.read more
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Synthetic vectors for gene delivery: An overview of their evolution depending on routes of administration.
Nawal Belmadi,Patrick Midoux,Pascal Loyer,Pascal Loyer,Catherine Passirani,Chantal Pichon,Tony Le Gall,Paul-Alain Jaffrès,Pierre Lehn,Tristan Montier +9 more
TL;DR: Various synthetic vectors for gene delivery are reviewed and the question of their biodistribution as a function of the route of administration is addressed and modifications to vectors structure and formulations necessary to overcome the major hurdles limiting the effectiveness of nucleic acid therapies are highlighted.
Journal ArticleDOI
Involvement of a Rac1-Dependent Macropinocytosis Pathway in Plasmid DNA Delivery by Electrotransfection.
Mao Mao,Liangli Wang,Chun-Chi Chang,Katheryn E. Rothenberg,Jianyong Huang,Yingxiao Wang,Brenton D. Hoffman,Paloma B. Liton,Fan Yuan +8 more
TL;DR: The findings suggested that electrotransfection of pDNA involved Rac1-dependent macropinocytosis, which could be decreased significantly by reducing temperature or treatment of cells with a pharmacological inhibitor of Rac1 and could be altered by changing Rac1 activity.
Journal ArticleDOI
3-BrPA eliminates human bladder cancer cells with highly oncogenic signatures via engagement of specific death programs and perturbation of multiple signaling and metabolic determinants.
Eumorphia G. Konstantakou,Gerassimos E. Voutsinas,Athanassios D. Velentzas,Aggeliki-Stefania Basogianni,Efthimios Paronis,Evangelos Balafas,Nikolaos Kostomitsopoulos,Konstantinos N. Syrigos,Ema Anastasiadou,Dimitrios J. Stravopodis +9 more
TL;DR: 3-BrPA, a halogenated derivative of pyruvate and historically considered inhibitor of glycolysis, is employed to eliminate bladder cancer cells with highly oncogenic molecular signatures to represent a promising agent for bladder cancer targeted therapy.
Journal ArticleDOI
Polymer nanocarriers for MicroRNA delivery
Chintan H. Kapadia,Benjamin Luo,Megan N. Dang,N’Dea S. Irvin-Choy,Danielle M. Valcourt,Emily S. Day +5 more
TL;DR: The challenges associated with miRNA mimic delivery are emphasized and the design and implementation of polymer nanocarriers to overcome these challenges are discussed, and a forward-looking perspective on the future clinical translation of polymer Nanomaterials as miRNA delivery vehicles is provided.
Journal ArticleDOI
Macropinocytosis of Bevacizumab by Glioblastoma Cells in the Perivascular Niche Affects their Survival
Gaelle Muller-Greven,Gaelle Muller-Greven,Cathleen R. Carlin,Monica E. Burgett,Monica E. Burgett,Manmeet Ahluwalia,Adam Lauko,Amy S. Nowacki,Cameron Herting,Maha A. Qadan,Maha A. Qadan,Markus Bredel,Steven A. Toms,Justin D. Lathia,Justin D. Lathia,Dolores Hambardzumyan,Jann N. Sarkaria,Petra Hamerlik,Candece L. Gladson +18 more
TL;DR: It is demonstrated that bevacizumab is internalized by Sox2+/CD44+-GBM tumor cells residing in the perivascular tumor niche, suggesting macropinocytosis of bevazumab promotes CD133+ cell survival, as does the autophagy induced by bevaccizumAB depletion of VEGF-A.
References
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Journal ArticleDOI
Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
Journal ArticleDOI
The fluid mosaic model of the structure of cell membranes.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.
Journal ArticleDOI
Regulated portals of entry into the cell
Sean D. Conner,Sandra L. Schmid +1 more
TL;DR: ‘Endocytosis’ encompasses several diverse mechanisms by which cells internalize macromolecules and particles into transport vesicles derived from the plasma membrane and must be viewed in a broader context than simple vesicular trafficking.
Journal ArticleDOI
Mechanisms of Endocytosis
TL;DR: What is known about mammalian endocytic mechanisms is reviewed, with focus on the cellular proteins that control these events, and the functional relevance of distinctendocytic pathways is discussed.
Book ChapterDOI
THE FLUID MOSAIC MODEL OF THE STRUCTURE OF CELL MEMBRANES Reprinted with permission from Science, Copyright AAA, 18 February 1972, Volume 175, pp. 720–731.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.