Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis.
TLDR
This review covers the well-recognized clathrin-mediated endocytosis (CME), phagocytotic, and macropinocyTosis in addition to the less addressed pathways that take place in lipid rafts, and summarizes the regulators associated with each uptake pathway.About:
This article is published in Molecular Therapy.The article was published on 2013-06-01 and is currently open access. It has received 247 citations till now. The article focuses on the topics: Endocytic vesicle & Endocytic cycle.read more
Citations
More filters
Journal ArticleDOI
Novel glycol chitosan-based polymeric gene carrier synthesized by a Michael addition reaction with low molecular weight polyethylenimine.
TL;DR: The results demonstrate that GMP, a hybrid polymer of glycol chitosan grafted with branched polyethylenimine, may serve as a promising vehicle for efficient gene delivery.
Journal ArticleDOI
Transfection Mechanisms of Polyplexes, Lipoplexes, and Stealth Liposomes in α5β1 Integrin Bearing DLD-1 Colorectal Cancer Cells
TL;DR: In this paper, the authors investigated the transfection mechanisms of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP)/1,2dioxyl-sn-glycero-3phosphoethanolamine (DOPE) lipoplexes, branched polyethylenimine (bPEI) polyplexes, and encapsulated in either PEGylated (stealth) nontargeted liposomes or PR_b peptide (targeted to α5
Journal ArticleDOI
Complexity in the therapeutic delivery of RNAi medicines: an analytical challenge
TL;DR: The purpose of this review is to reflect on the complexity in the therapeutic delivery of RNA interference-based drugs emerging from the recent clinical experiences and report the actual technological and analytical advances introduced to solve it.
Journal ArticleDOI
Di-Peptide-Modified Gemini Surfactants as Gene Delivery Vectors: Exploring the Role of the Alkyl Tail in Their Physicochemical Behavior and Biological Activity.
Mays Al-Dulaymi,Jackson M. Chitanda,Waleed Mohammed-Saeid,Hessamaddin Younesi Araghi,Ronald E. Verrall,Pawel Grochulski,Pawel Grochulski,Ildiko Badea +7 more
TL;DR: The results show that the length and nature of the tail of the gemini surfactants play an important role in determining the transgene efficiency of the delivery system and demonstrated here that the interplay between the headgroup and the nature of tail is specific to each series.
Journal ArticleDOI
A review of the tortuous path of nonviral gene delivery and recent progress.
TL;DR: In this paper, a review of non-viral gene delivery systems is presented, focusing on the current evolving state of nonviral delivery systems and their future perspectives, and emphasizing the recent advancements aimed at enhancing the current nonvirus approaches.
References
More filters
Journal ArticleDOI
Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
Journal ArticleDOI
The fluid mosaic model of the structure of cell membranes.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.
Journal ArticleDOI
Regulated portals of entry into the cell
Sean D. Conner,Sandra L. Schmid +1 more
TL;DR: ‘Endocytosis’ encompasses several diverse mechanisms by which cells internalize macromolecules and particles into transport vesicles derived from the plasma membrane and must be viewed in a broader context than simple vesicular trafficking.
Journal ArticleDOI
Mechanisms of Endocytosis
TL;DR: What is known about mammalian endocytic mechanisms is reviewed, with focus on the cellular proteins that control these events, and the functional relevance of distinctendocytic pathways is discussed.
Book ChapterDOI
THE FLUID MOSAIC MODEL OF THE STRUCTURE OF CELL MEMBRANES Reprinted with permission from Science, Copyright AAA, 18 February 1972, Volume 175, pp. 720–731.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.