Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis.
TLDR
This review covers the well-recognized clathrin-mediated endocytosis (CME), phagocytotic, and macropinocyTosis in addition to the less addressed pathways that take place in lipid rafts, and summarizes the regulators associated with each uptake pathway.About:
This article is published in Molecular Therapy.The article was published on 2013-06-01 and is currently open access. It has received 247 citations till now. The article focuses on the topics: Endocytic vesicle & Endocytic cycle.read more
Citations
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Journal Article
Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes
Joanna Rejman,Massimo Conese +1 more
TL;DR: In this article, the authors investigated the effects of inhibitors of clathrin-mediated endocytosis (chlorpromazine and K(+) depletion) and of caveolae-mediated uptake (filipin and genistein) on internalization of FITC-poly-l-lysine-labeled DOTAP/DNA lipoplexes and PEI/DNA polyplexes by A549 pneumocytes and HeLa cells and on the transfection efficiencies of these complexes with the luciferase gene.
Journal ArticleDOI
Cellular uptake and trafficking of antisense oligonucleotides
TL;DR: Although PS-ASOs function in both the cytoplasm and nucleus, localization to different subcellular regions can affect their therapeutic potency and the main proteins involved in these processes have been identified and intracellular sites in which PS ASOs are active, or inactive, cataloged.
Journal ArticleDOI
Overcoming Nonviral Gene Delivery Barriers: Perspective and Future
TL;DR: This work presents the existing delivery barriers and summarize current vector-specific strategies to overcome said barriers, and focuses on the gene delivery barriers associated with the two most common classes of nonviral vectors, cationic-based lipids and polymers.
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Nonviral cancer gene therapy: Delivery cascade and vector nanoproperty integration.
Zhuxian Zhou,Xiangrui Liu,Dingcheng Zhu,Yue Wang,Zhen Zhang,Xuefei Zhou,Nasha Qiu,Xuesi Chen,Youqing Shen +8 more
TL;DR: This review analyzes the cancer gene‐delivery cascade and the barriers, the needed nanopro properties and the current strategies for overcoming these barriers, and outlines PEGylation, surface‐charge, size, and stability dilemmas in vector nanoproperties to efficiently accomplish the cancer genes delivery cascade.
Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells
Mark T. Howes,Matthew Kirkham,James D. Riches,Katia Cortese,Piers J. Walser,Fiona Simpson,Michelle M. Hill,Alun Jones,Richard Lundmark,Margaret R. Lindsay,Delia J. Hernández-Deviez,Gordana Hadzic,Adam McCluskey,Rumasia Bashir,Libin Liu,Paul F. Pilch,Harvey T. McMahon,Phillip J. Robinson,John F. Hancock,Satyajit Mayor,Robert G. Parton +20 more
TL;DR: In this article, the authors provided the first quantitative ultrastructural analysis and molecular characterization of the major endocytic pathway in fibroblasts, a pathway that provides rapid membrane turnover at the leading edge of migrating cells.
References
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GPI-anchored proteins are delivered to recycling endosomes via a distinct cdc42-regulated, clathrin-independent pinocytic pathway
TL;DR: It is shown that multiple glycosylphosphatidylinositol-anchored proteins (GPI-APs) are endocytosed to the recycling endosomal compartment but not to the Golgi via a nonclathrin, noncaveolae mediated pathway.
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Dynamin at the Neck of Caveolae Mediates Their Budding to Form Transport Vesicles by GTP-driven Fission from the Plasma Membrane of Endothelium
TL;DR: Through its ability to oligomerize, dynamin appears to form a structural collar around the neck of caveolae that hydrolyzes GTP to mediate internalization via the fission of Caveolae from the plasma membrane to form free transport vesicles.
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Clathrin-mediated internalization is essential for sustained EGFR signaling but dispensable for degradation.
Sara Sigismund,Elisabetta Argenzio,Daniela Tosoni,Elena Cavallaro,Simona Polo,Pier Paolo Di Fiore +5 more
TL;DR: It is reported that EGFRs internalized via CME are not targeted for degradation, but instead are recycled to the cell surface, which has profound implications for signaling, as by skewing EGFR fate toward recycling rather than degradation, CME prolongs the duration of signaling.
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HIV Enters Cells via Endocytosis and Dynamin-Dependent Fusion with Endosomes
TL;DR: It is shown that HIV-1 underwent receptor-mediated internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes.
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Macropinocytosis: an endocytic pathway for internalising large gulps
Jet Phey Lim,Paul A. Gleeson +1 more
TL;DR: The general characteristics of macropinocytosis are reviewed, some of the regulators of this pathway, which have been identified to date, are described and strategies to explore the relevance of this endocytotic pathway in vivo are highlighted.