Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis.
TLDR
This review covers the well-recognized clathrin-mediated endocytosis (CME), phagocytotic, and macropinocyTosis in addition to the less addressed pathways that take place in lipid rafts, and summarizes the regulators associated with each uptake pathway.About:
This article is published in Molecular Therapy.The article was published on 2013-06-01 and is currently open access. It has received 247 citations till now. The article focuses on the topics: Endocytic vesicle & Endocytic cycle.read more
Citations
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Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes
Joanna Rejman,Massimo Conese +1 more
TL;DR: In this article, the authors investigated the effects of inhibitors of clathrin-mediated endocytosis (chlorpromazine and K(+) depletion) and of caveolae-mediated uptake (filipin and genistein) on internalization of FITC-poly-l-lysine-labeled DOTAP/DNA lipoplexes and PEI/DNA polyplexes by A549 pneumocytes and HeLa cells and on the transfection efficiencies of these complexes with the luciferase gene.
Journal ArticleDOI
Cellular uptake and trafficking of antisense oligonucleotides
TL;DR: Although PS-ASOs function in both the cytoplasm and nucleus, localization to different subcellular regions can affect their therapeutic potency and the main proteins involved in these processes have been identified and intracellular sites in which PS ASOs are active, or inactive, cataloged.
Journal ArticleDOI
Overcoming Nonviral Gene Delivery Barriers: Perspective and Future
TL;DR: This work presents the existing delivery barriers and summarize current vector-specific strategies to overcome said barriers, and focuses on the gene delivery barriers associated with the two most common classes of nonviral vectors, cationic-based lipids and polymers.
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Nonviral cancer gene therapy: Delivery cascade and vector nanoproperty integration.
Zhuxian Zhou,Xiangrui Liu,Dingcheng Zhu,Yue Wang,Zhen Zhang,Xuefei Zhou,Nasha Qiu,Xuesi Chen,Youqing Shen +8 more
TL;DR: This review analyzes the cancer gene‐delivery cascade and the barriers, the needed nanopro properties and the current strategies for overcoming these barriers, and outlines PEGylation, surface‐charge, size, and stability dilemmas in vector nanoproperties to efficiently accomplish the cancer genes delivery cascade.
Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells
Mark T. Howes,Matthew Kirkham,James D. Riches,Katia Cortese,Piers J. Walser,Fiona Simpson,Michelle M. Hill,Alun Jones,Richard Lundmark,Margaret R. Lindsay,Delia J. Hernández-Deviez,Gordana Hadzic,Adam McCluskey,Rumasia Bashir,Libin Liu,Paul F. Pilch,Harvey T. McMahon,Phillip J. Robinson,John F. Hancock,Satyajit Mayor,Robert G. Parton +20 more
TL;DR: In this article, the authors provided the first quantitative ultrastructural analysis and molecular characterization of the major endocytic pathway in fibroblasts, a pathway that provides rapid membrane turnover at the leading edge of migrating cells.
References
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A novel mechanism is involved in cationic lipid-mediated functional siRNA delivery.
TL;DR: In this article, the authors investigated the mechanisms by which a cationic lipid carrier mediates siRNA delivery into mammalian cells and showed that approximately 95% of siRNA lipoplexes enter the cells through endocytosis and persist in endolysosomes for a prolonged period of time.
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Reggie-1 and reggie-2, two cell surface proteins expressed by retinal ganglion cells during axon regeneration
TL;DR: Reggie-1 and reggie-2 are identified as neuronal surface proteins, present on newly differentiated ganglion cells at the retinal margin and which are reexpressed in mature ganglION cells upon injury and during axonal regeneration.
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Efficient polyethylenimine-mediated gene delivery proceeds via a caveolar pathway in HeLa cells.
TL;DR: The impact of targeting two distinct endocytic routes-the caveolar and clathrin pathways-on polyethylenimine-mediated gene delivery in HeLa cells is examined to demonstrate that uptake mechanism and subsequentendocytic processing are important design parameters for gene delivery materials.
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The characteristics, cellular uptake and intracellular trafficking of nanoparticles made of hydrophobically-modified chitosan
Ya Ling Chiu,Yi Cheng Ho,Yu-Ming Chen,Shu Fen Peng,Cherng-Jyh Ke,Ko Jie Chen,Fwu Long Mi,Hsing-Wen Sung +7 more
TL;DR: The results obtained in the intracellular trafficking study showed that NPCS NPs entered cells via caveolae and transiently localized to caveosomes before trafficking to the endosomal pathway, suggesting that the prepared NCPS NPs may serve as a carrier for intrACEllular delivery of therapeutic agents.
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Gulping rather than sipping: macropinocytosis as a way of virus entry.
Jason Mercer,Ari Helenius +1 more
TL;DR: Macropinocytosis has emerged as a major endocytic mechanism in the cell entry of animal viruses, and recent studies show that this strategy is used by a variety of enveloped and non-enveloped viruses.