Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis.
TLDR
This review covers the well-recognized clathrin-mediated endocytosis (CME), phagocytotic, and macropinocyTosis in addition to the less addressed pathways that take place in lipid rafts, and summarizes the regulators associated with each uptake pathway.About:
This article is published in Molecular Therapy.The article was published on 2013-06-01 and is currently open access. It has received 247 citations till now. The article focuses on the topics: Endocytic vesicle & Endocytic cycle.read more
Citations
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Journal ArticleDOI
Overcoming Blood-Brain Barrier Resistance: Implications for Extracellular Vesicle-Mediated Drug Brain Delivery
TL;DR: The BBB structural and functional resistance, as well as potential avenues to overcome this challenge using EVs as drug delivery vehicles into the brain are discussed.
Journal ArticleDOI
Exosomal transmission of viruses, a two-edged biological sword
Narges Mardi,Sanya Haiaty,Reza Rahbarghazi,Halimeh Mobarak,Morteza Milani,Amir Zarebkohan,Mohammad Zamam Nouri +6 more
TL;DR: In this article , a review of challenges and opportunities related to the exosomal delivery of viruses in terms of viral infections and public health is presented. But the authors do not consider the role of exosomes in the transmission of viruses.
Journal ArticleDOI
Efficient gene delivery to primary human retinal pigment epithelial cells: The innate and acquired properties of vectors.
Nooshin Tasharrofi,Nooshin Tasharrofi,Fatemeh Kouhkan,Masoud Soleimani,Zahra-Soheila Soheili,Fatemeh Atyabi,Hamid Akbari Javar,Farid Abedin Dorkoosh +7 more
TL;DR: V vectors with different characteristics differing in mixing orders of pDNA, PEI polymer, and PLGA/PEI or PLGA nanoparticles were prepared and characterized and showed no cytotoxicity and were able to protect pDNA from premature release and degradation in extracellular media.
Journal ArticleDOI
Lysine functionalized nanodiamonds as gene carriers - Investigation of internalization pathways and intracellular trafficking
Saniya Alwani,QingYun Hua,Safa Iftikhar,N. Appathurai,Deborah Michel,Chithra Karunakaran,Ildiko Badea +6 more
TL;DR: These findings are the basis of further optimization of amino acid functionalized NDs towards increase of gene delivery efficacy through targeted internalization pathways and overcoming intracellular challenges.
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Membrane domain formation—a key factor for targeted intracellular drug delivery
TL;DR: It is proposed that clustering induced formation of plasma membrane microdomains enriched in receptors, sphingolipids, and inositol lipids, leads to membrane bending which functions as the onset of RME.
References
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Journal ArticleDOI
Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
Journal ArticleDOI
The fluid mosaic model of the structure of cell membranes.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.
Journal ArticleDOI
Regulated portals of entry into the cell
Sean D. Conner,Sandra L. Schmid +1 more
TL;DR: ‘Endocytosis’ encompasses several diverse mechanisms by which cells internalize macromolecules and particles into transport vesicles derived from the plasma membrane and must be viewed in a broader context than simple vesicular trafficking.
Journal ArticleDOI
Mechanisms of Endocytosis
TL;DR: What is known about mammalian endocytic mechanisms is reviewed, with focus on the cellular proteins that control these events, and the functional relevance of distinctendocytic pathways is discussed.
Book ChapterDOI
THE FLUID MOSAIC MODEL OF THE STRUCTURE OF CELL MEMBRANES Reprinted with permission from Science, Copyright AAA, 18 February 1972, Volume 175, pp. 720–731.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.