Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis.
TLDR
This review covers the well-recognized clathrin-mediated endocytosis (CME), phagocytotic, and macropinocyTosis in addition to the less addressed pathways that take place in lipid rafts, and summarizes the regulators associated with each uptake pathway.About:
This article is published in Molecular Therapy.The article was published on 2013-06-01 and is currently open access. It has received 247 citations till now. The article focuses on the topics: Endocytic vesicle & Endocytic cycle.read more
Citations
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Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes
Joanna Rejman,Massimo Conese +1 more
TL;DR: In this article, the authors investigated the effects of inhibitors of clathrin-mediated endocytosis (chlorpromazine and K(+) depletion) and of caveolae-mediated uptake (filipin and genistein) on internalization of FITC-poly-l-lysine-labeled DOTAP/DNA lipoplexes and PEI/DNA polyplexes by A549 pneumocytes and HeLa cells and on the transfection efficiencies of these complexes with the luciferase gene.
Journal ArticleDOI
Cellular uptake and trafficking of antisense oligonucleotides
TL;DR: Although PS-ASOs function in both the cytoplasm and nucleus, localization to different subcellular regions can affect their therapeutic potency and the main proteins involved in these processes have been identified and intracellular sites in which PS ASOs are active, or inactive, cataloged.
Journal ArticleDOI
Overcoming Nonviral Gene Delivery Barriers: Perspective and Future
TL;DR: This work presents the existing delivery barriers and summarize current vector-specific strategies to overcome said barriers, and focuses on the gene delivery barriers associated with the two most common classes of nonviral vectors, cationic-based lipids and polymers.
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Nonviral cancer gene therapy: Delivery cascade and vector nanoproperty integration.
Zhuxian Zhou,Xiangrui Liu,Dingcheng Zhu,Yue Wang,Zhen Zhang,Xuefei Zhou,Nasha Qiu,Xuesi Chen,Youqing Shen +8 more
TL;DR: This review analyzes the cancer gene‐delivery cascade and the barriers, the needed nanopro properties and the current strategies for overcoming these barriers, and outlines PEGylation, surface‐charge, size, and stability dilemmas in vector nanoproperties to efficiently accomplish the cancer genes delivery cascade.
Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells
Mark T. Howes,Matthew Kirkham,James D. Riches,Katia Cortese,Piers J. Walser,Fiona Simpson,Michelle M. Hill,Alun Jones,Richard Lundmark,Margaret R. Lindsay,Delia J. Hernández-Deviez,Gordana Hadzic,Adam McCluskey,Rumasia Bashir,Libin Liu,Paul F. Pilch,Harvey T. McMahon,Phillip J. Robinson,John F. Hancock,Satyajit Mayor,Robert G. Parton +20 more
TL;DR: In this article, the authors provided the first quantitative ultrastructural analysis and molecular characterization of the major endocytic pathway in fibroblasts, a pathway that provides rapid membrane turnover at the leading edge of migrating cells.
References
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Virus entry by macropinocytosis
Jason Mercer,Ari Helenius +1 more
TL;DR: The molecular machinery involved in macropinocytosis is reviewed and what is known about its role in virus entry is described.
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Dynamin-mediated Internalization of Caveolae
TL;DR: Functional in vivo studies as well as ultrastructural and biochemical analyses indicate that dynamin mediates both clathrin-dependent endocytosis and the internalization of caveolae in mammalian cells.
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Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling
Mirkka Koivusalo,Christopher Welch,Hisayoshi Hayashi,Cameron C. Scott,Moshe Kim,R. Todd Alexander,Nicolas Touret,Klaus M. Hahn,Sergio Grinstein +8 more
TL;DR: Inhibitors of Na+/H+ exchange proteins block macropinocytosis by lowering the pH near the plasma membrane, which in turn inhibits actin remodeling by Rho family GTPases.
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Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes
TL;DR: Fluorescence colocalization studies with a lysosomal marker, AlexaFluor-dextran, revealed that polyplexes taken up by clathrin-mediated endocytosis are targeted to the lysOSomal compartment for degradation, while the polyplexed via caveolae escape this compartment, permitting efficient transfection.
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Cellular uptake of arginine-rich peptides: roles for macropinocytosis and actin rearrangement.
Ikuhiko Nakase,Miki Niwa,Toshihide Takeuchi,Kazuhiro Sonomura,Noriko Kawabata,Yukihiro Koike,Masanori Takehashi,Seigo Tanaka,Kunihiro Ueda,Jeremy C. Simpson,Arwyn Tomos Jones,Yukio Sugiura,Shiroh Futaki,Shiroh Futaki +13 more
TL;DR: It is shown that the uptake of octa-arginine (R8) peptide by HeLa cells was significantly suppressed by the macropinocytosis inhibitor ethylisopropylamiloride (EIPA) and the F-actin polymerization inhibitor cytochalasin D, suggesting a role for macrop inocytotic in the uptake and suggesting that penetratin and R8 peptides have distinct internalization mechanisms.